湖北农业科学
湖北農業科學
호북농업과학
Hubei Agricultural Sciences
2015年
17期
4164-4167,4172
,共5页
袁俊超%吕宝%屈敦妮%董淑君%徐超%郭诗仪%熊燕飞
袁俊超%呂寶%屈敦妮%董淑君%徐超%郭詩儀%熊燕飛
원준초%려보%굴돈니%동숙군%서초%곽시의%웅연비
木质素%天牛幼虫%pH%温度%协同性%烟曲霉菌%沤肥
木質素%天牛幼蟲%pH%溫度%協同性%煙麯黴菌%漚肥
목질소%천우유충%pH%온도%협동성%연곡매균%구비
lignin%longicorn larva%pH%temperature%cooperativity%A spergillus fumigatus%composting
天牛幼虫肠道菌群具有分解木质纤维的功能。本研究以木质纤维为唯一碳源,从天牛幼虫肠道中筛选出三株对木纤维具有降解能力的菌株,对其中的烟曲霉YSITB I菌株降解木纤维中木质素能力及降解条件进行研究,并对三种菌株降解木纤维中木质素的协同作用进行了初步观察。结果显示,烟曲霉YSITB I菌株具有较好的木质素降解作用,在30℃、pH值6.8条件下,对构树木质纤维中木质素的降解率可达56.9%;枯草芽孢杆菌YSITBII和烟曲霉YSITB I协同作用可提高木质素降解效率至64.8%;烟曲霉YSITB I 15d降解木纤维效率高于40%。烟曲霉对木质纤维的降解作用达到甚至高于模式菌-黄包原毛平革菌,降解木纤维程度较高,用于木质纤维脱木质素或秸秆降解沤肥具有很好的应用前景。
天牛幼蟲腸道菌群具有分解木質纖維的功能。本研究以木質纖維為唯一碳源,從天牛幼蟲腸道中篩選齣三株對木纖維具有降解能力的菌株,對其中的煙麯黴YSITB I菌株降解木纖維中木質素能力及降解條件進行研究,併對三種菌株降解木纖維中木質素的協同作用進行瞭初步觀察。結果顯示,煙麯黴YSITB I菌株具有較好的木質素降解作用,在30℃、pH值6.8條件下,對構樹木質纖維中木質素的降解率可達56.9%;枯草芽孢桿菌YSITBII和煙麯黴YSITB I協同作用可提高木質素降解效率至64.8%;煙麯黴YSITB I 15d降解木纖維效率高于40%。煙麯黴對木質纖維的降解作用達到甚至高于模式菌-黃包原毛平革菌,降解木纖維程度較高,用于木質纖維脫木質素或秸稈降解漚肥具有很好的應用前景。
천우유충장도균군구유분해목질섬유적공능。본연구이목질섬유위유일탄원,종천우유충장도중사선출삼주대목섬유구유강해능력적균주,대기중적연곡매YSITB I균주강해목섬유중목질소능력급강해조건진행연구,병대삼충균주강해목섬유중목질소적협동작용진행료초보관찰。결과현시,연곡매YSITB I균주구유교호적목질소강해작용,재30℃、pH치6.8조건하,대구수목질섬유중목질소적강해솔가체56.9%;고초아포간균YSITBII화연곡매YSITB I협동작용가제고목질소강해효솔지64.8%;연곡매YSITB I 15d강해목섬유효솔고우40%。연곡매대목질섬유적강해작용체도심지고우모식균-황포원모평혁균,강해목섬유정도교고,용우목질섬유탈목질소혹갈간강해구비구유흔호적응용전경。
The intestinal flora of longicorn larva could decompose lignocellulose. Using lignocellulose as the unique carbon source, three strains with the ablity of decomposing lignin were isolated from intestine of the larva. The degradation ability and degradation conditions of lignin in lignocellulose of Aspergillus fumigatus YSITBI as well as the synergistic action of the three strains were studied. The results showed that A . fumigatus YSITBI was good at decomposing lignin, under the condi-tions of 30 ℃, pH 6.8, the degradability of lignin in lignocellulose reached to 56.9%. The cooperation of Bacillus subtilis YSITBII and A . fumigatus YSITBI could improve the degradability of lignin to 64.8%. The degradability of A . fumigatus YSITBI achieved or was even higher than the model bacteria Phanerochaete chrysosporium, thus had a very good prospect in decomposing the lignin from lignocellulose or decomposing straws for fertilizer.