CAJ | 학술논문

目的：探讨当归红芪水煎剂对糖尿病大鼠肾脏的保护作用。方法：将72只Wistar大鼠随机分为正常对照组12只，其余60只大鼠，给予一次性大剂量腹腔注射链脲佐菌素（STZ）复制糖尿病模型。造模成功后将糖尿病大鼠随机分为厄贝沙坦组（给予17.5mg?kg-1?d-1灌胃），当归红芪低剂量组(给予1.8g?kg-1?d-1灌胃），当归红芪中剂量组(给予3.6g?kg-1?d-1灌胃），当归红芪高剂量组（给予7.2g?kg-1?d-1灌胃），模型组（给予等量生理盐水灌胃），每组12只。4周后测空腹血糖（FBG）；用代谢笼留大鼠24h尿，考马斯亮蓝G-250法测定24h尿蛋白含量（24 h Upro）；解剖大鼠，取双肾，称重，计算肾脏指数；采用HE染色观察各组大鼠肾组织的病理变化。结果：与正常对照组比较模型组大鼠FBG、24 hUpro、肾重指数均明显升高（P＜0.01）。与模型组比较，当归红芪中、高剂量组空腹血糖有不同程度的降低（P＜0.05）；西药组及三个当归红芪组24 h Upro均有所下降（P＜0.05）；西药组及当归红芪中、高剂量组肾重指数明显下降（P＜0.05）；西药组及当归红芪中、高剂量组肾脏病理变化较轻（P＜0.05）。结论：当归红芪水煎剂可以降低DM大鼠的血糖、减少蛋白尿、减轻肾脏病理损害，从而可以延缓糖尿病肾病的进展。
목적：탐토당귀홍기수전제대당뇨병대서신장적보호작용。방법：장72지Wistar대서수궤분위정상대조조12지，기여60지대서，급여일차성대제량복강주사련뇨좌균소（STZ）복제당뇨병모형。조모성공후장당뇨병대서수궤분위액패사탄조（급여17.5mg?kg-1?d-1관위），당귀홍기저제량조(급여1.8g?kg-1?d-1관위），당귀홍기중제량조(급여3.6g?kg-1?d-1관위），당귀홍기고제량조（급여7.2g?kg-1?d-1관위），모형조（급여등량생리염수관위），매조12지。4주후측공복혈당（FBG）；용대사롱류대서24h뇨，고마사량람G-250법측정24h뇨단백함량（24 h Upro）；해부대서，취쌍신，칭중，계산신장지수；채용HE염색관찰각조대서신조직적병리변화。결과：여정상대조조비교모형조대서FBG、24 hUpro、신중지수균명현승고（P＜0.01）。여모형조비교，당귀홍기중、고제량조공복혈당유불동정도적강저（P＜0.05）；서약조급삼개당귀홍기조24 h Upro균유소하강（P＜0.05）；서약조급당귀홍기중、고제량조신중지수명현하강（P＜0.05）；서약조급당귀홍기중、고제량조신장병리변화교경（P＜0.05）。결론：당귀홍기수전제가이강저DM대서적혈당、감소단백뇨、감경신장병리손해，종이가이연완당뇨병신병적진전。
Objective:To investigate effeccts of the Danggui Hongqi decoction on kidney of experimental diabetic rats. Methods: 72 Wistar rats were randomly divided into control group 12, the remaining 60 rats were administered bolus intraperitoneal injection of streptozotocin (STZ) diabetic copy. Later, successful modeling of diabetic rats were randomly divided into irbesartan group (given 17.5mg?kg-1?d-1 orally), Danggui Hongqi low dose group (given1.8 g?kg-1?d-1 orally), Danggui Hongqi dose group (given 3.6 g?kg-1?d-1 orally), Danggui Hongqi high dose group (given 7.2 g?kg-1?d-1 orally), model group (given normal saline orally), each 12. Four weeks after fasting blood glucose (FBG) was done, with 24h urine of rats, and Determinating 24h urinary protein (24h Upro); dissect rats for kidneys, weighing, calculating kidney index; HE staining for renal tissue pathological changes. Results: Compared with normal control group of rats FBG model group, 24hUpro, kidney weight index were significantly increased (P<0.01). Compared with the model group, danggui hongqi middle and high dose groups have different levels of fasting blood glucose lowering(P<0.05). Western medicine group and three Danggui Hongqi 24h Upro group had decreased(P<0.05); Western medicine group and Danggui Hongqi middle dose group and high dose group significantly decreased kidney weight index(P<0.05); Western medicine group and Danggui Hongqi middle dose group and high dose group mild were found renal pathological changes (P<0.05). Conclusion: Danggui Hongqi decoction can reduce the blood sugar, reduce proteinuria, in DM rats can relieve kidney pathological damage, and can delay the progress of diabetic nephropathy.