重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
26期
3609-3612
,共4页
肝硬化%胆汁淤积,肝内%S-腺苷蛋氨酸
肝硬化%膽汁淤積,肝內%S-腺苷蛋氨痠
간경화%담즙어적,간내%S-선감단안산
liver cirrhosis%cholestasis,intrahepatic%S-adenosyl methionine
目的:研究 S-腺苷蛋氨酸(SAMe)对奈乙硫氰酸酯(ANIT)诱发大鼠药物型胆汁淤积性肝病肝纤维化的保护作用。方法将18只 SD 大鼠分为3组:A 组(空白对照组)、B 组(造模+NS)、C 组(造模+SAMe),每组各6只,各组大鼠腹腔注射后72 h 处理。采用全自动生化分析仪检测各组肝脏血清学指标,并取肝脏组织行 HE 染色及 MASSON 染色观察肝脏病理组织学变化及胶原含量改变。结果C 组大鼠的血清学指标谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBiL)、直接胆红素(DBiL)、间接胆红素(IBiL)、谷胺酰转肽酶(γ-GGT)、碱性磷酸酶(ALP)、总胆汁酸(TBA)明显低于 B 组,3组之间比较差异均有统计学意义(P <0.05);但3组之间总胆固醇(Chol)水平差异无统计学意义(P >0.05)。C 组大鼠的胶原纤维含量及胶原纤维面积比均明显低于 B 组,差异有统计学意义(P <0.05)。结论SAMe 能通过改善药物型胆汁淤积性肝病的肝功能,降低胶原纤维含量及面积,进一步保护肝脏组织。
目的:研究 S-腺苷蛋氨痠(SAMe)對奈乙硫氰痠酯(ANIT)誘髮大鼠藥物型膽汁淤積性肝病肝纖維化的保護作用。方法將18隻 SD 大鼠分為3組:A 組(空白對照組)、B 組(造模+NS)、C 組(造模+SAMe),每組各6隻,各組大鼠腹腔註射後72 h 處理。採用全自動生化分析儀檢測各組肝髒血清學指標,併取肝髒組織行 HE 染色及 MASSON 染色觀察肝髒病理組織學變化及膠原含量改變。結果C 組大鼠的血清學指標穀氨痠氨基轉移酶(ALT)、天鼕氨痠氨基轉移酶(AST)、總膽紅素(TBiL)、直接膽紅素(DBiL)、間接膽紅素(IBiL)、穀胺酰轉肽酶(γ-GGT)、堿性燐痠酶(ALP)、總膽汁痠(TBA)明顯低于 B 組,3組之間比較差異均有統計學意義(P <0.05);但3組之間總膽固醇(Chol)水平差異無統計學意義(P >0.05)。C 組大鼠的膠原纖維含量及膠原纖維麵積比均明顯低于 B 組,差異有統計學意義(P <0.05)。結論SAMe 能通過改善藥物型膽汁淤積性肝病的肝功能,降低膠原纖維含量及麵積,進一步保護肝髒組織。
목적:연구 S-선감단안산(SAMe)대내을류청산지(ANIT)유발대서약물형담즙어적성간병간섬유화적보호작용。방법장18지 SD 대서분위3조:A 조(공백대조조)、B 조(조모+NS)、C 조(조모+SAMe),매조각6지,각조대서복강주사후72 h 처리。채용전자동생화분석의검측각조간장혈청학지표,병취간장조직행 HE 염색급 MASSON 염색관찰간장병리조직학변화급효원함량개변。결과C 조대서적혈청학지표곡안산안기전이매(ALT)、천동안산안기전이매(AST)、총담홍소(TBiL)、직접담홍소(DBiL)、간접담홍소(IBiL)、곡알선전태매(γ-GGT)、감성린산매(ALP)、총담즙산(TBA)명현저우 B 조,3조지간비교차이균유통계학의의(P <0.05);단3조지간총담고순(Chol)수평차이무통계학의의(P >0.05)。C 조대서적효원섬유함량급효원섬유면적비균명현저우 B 조,차이유통계학의의(P <0.05)。결론SAMe 능통과개선약물형담즙어적성간병적간공능,강저효원섬유함량급면적,진일보보호간장조직。
Objective To study the protective effects of S-adenosyl methionine(SAMe)on liver fibrosis of rats with cholesta-sis induced by alpha-naphthyliso thiocyanate.Methods 18 SD rats were randomly divided into three groups,the group A (blank control group),B [model+ normal saline(NS)]and C (model + SAMe),6 rats in each group.The rats in each group were sacri-ficed at 72 h after intraperitoneal injection.Serum levels of liver indicators in each group were detected by the full automatic bio-chemical analyzer.The liver tissue was taken for conducting HE staining and MASSON staining and observing the pathological changes of livers.Results Compared with the group B,the serum levels of ALT,AST,TBiL,DBiL,IBiL,R-GGT,ALP and TBA in the group C were significantly decreased,the differences among 3 groups were statistically significant(P <0.05);but the total Chol level had no statistical difference among 3 groups(P >0.05).The collagen fibers content (1 75.1 ±127.9)and the collagen fibers area ratio(14.1±6.4)in the group C were significantly lower than those in the group B,the difference was statistically significant (P <0.05).Conclusion SAMe can decrease the content and area of collagen fibers and further protect the liver tissue by improving the liver function in drug induced cholestasis liver disease.