CAJ | 학술논문

目的：对比分析睾丸与附睾精子行卵胞浆内单精子注射术(ICSI)的结局及精子 DNA 完整率(用精子 DNA 断裂指数 DFI 表示)的差异。方法对来该院生殖中心就诊的183例梗阻性无精子症患者行 ICSI,将患者根据取精途径的不同分为两组,其中经皮附睾穿刺抽吸术(PESA)取精80个周期,经睾丸穿刺抽吸术(TESA)取精103个周期,对比两组精子 DNA 完整率及行 ICSI 的结局。结果两种方法精子 DNA 完整率和行 ICSI 时受精率、卵裂率、优胚率、妊娠率比较,差异无统计学意义(P >0.05)。结论睾丸及附睾精子的 DNA 完整率及行 ICSI 结局无显著差异,临床医生可根据个人经验或患者意愿选取精子行 ICSI助孕。
목적：대비분석고환여부고정자행란포장내단정자주사술(ICSI)적결국급정자 DNA 완정솔(용정자 DNA 단렬지수 DFI 표시)적차이。방법대래해원생식중심취진적183례경조성무정자증환자행 ICSI,장환자근거취정도경적불동분위량조,기중경피부고천자추흡술(PESA)취정80개주기,경고환천자추흡술(TESA)취정103개주기,대비량조정자 DNA 완정솔급행 ICSI 적결국。결과량충방법정자 DNA 완정솔화행 ICSI 시수정솔、란렬솔、우배솔、임신솔비교,차이무통계학의의(P >0.05)。결론고환급부고정자적 DNA 완정솔급행 ICSI 결국무현저차이,림상의생가근거개인경험혹환자의원선취정자행 ICSI조잉。
Objective To comparative analysis the intracytoplasmic sperm injection (ICSI)result and rate of sperm DNA in-tegrity (DNA fragmentation index,DFI)about testicular and epididymis sperm.Methods Totally 183 obstructed azoospermia pa-tients were choosed to use ICSI.80 cycles by PESA and 103 cycles by TESA,compared two groups of sperm DNA integrity rate and ICSI outcome.Results Sperm DNA integrity rate,fertilization rate,cleavage rate,good-qualityembryo rate and pregnancy rate com-pared with no difference by ICSI(P >0.05).Conclusion DNA integrity rate and ICSI outcomes of the testis and epididymis sperm have no significant differences,clinicians can be based on personal experiences or patients,wills to select sperm for ICSI.