CAJ | 학술논문

目的：探讨电针大肠俞募穴治疗功能性便秘的作用机制。方法32只小鼠随机分为对照组、模型组、针刺组和 EGC 抑制组。采用复方地芬诺酯灌胃造模法，电针刺激天枢、大肠俞，每次30 min，共治疗5次。运用光镜、电镜、免疫组化及原位杂交技术，观察电针治疗前后结肠组织上皮细胞形态学改变，结肠组织中 GDNF 蛋白及 mRNA 表达水平。结果与对照组比较，模型组结肠组织 GDNF 蛋白及 mRNA 表达水平显著降低（P ＜0．01）。电针刺激大肠俞募穴可提高结肠中 GDNF 蛋白及其 mRNA 的表达水平（P ＜0．05），光镜和电镜发现针刺可修复受损上皮细胞，进而改善肠道传输功能。EGC 抑制组结肠中GDNF 蛋白表达与模型组比较无显著性差异（P ＞0．05），与针刺组比较显著降低（P ＜0．05），GDNF mRNA 的表达水平高于模型组（P ＜0．05），低于针刺组，但差异无统计学意义（P ＞0．05）。光镜和电镜下 EGC 抑制组结肠组织形态改变较为严重。结论电针刺激大肠俞募穴可提高 EGC 细胞中 GDNF 蛋白及其 mRNA 的表达水平，修复受损结肠上皮细胞，进而改善肠道传输功能。
목적：탐토전침대장유모혈치료공능성편비적작용궤제。방법32지소서수궤분위대조조、모형조、침자조화 EGC 억제조。채용복방지분낙지관위조모법，전침자격천추、대장유，매차30 min，공치료5차。운용광경、전경、면역조화급원위잡교기술，관찰전침치료전후결장조직상피세포형태학개변，결장조직중 GDNF 단백급 mRNA 표체수평。결과여대조조비교，모형조결장조직 GDNF 단백급 mRNA 표체수평현저강저（P ＜0．01）。전침자격대장유모혈가제고결장중 GDNF 단백급기 mRNA 적표체수평（P ＜0．05），광경화전경발현침자가수복수손상피세포，진이개선장도전수공능。EGC 억제조결장중GDNF 단백표체여모형조비교무현저성차이（P ＞0．05），여침자조비교현저강저（P ＜0．05），GDNF mRNA 적표체수평고우모형조（P ＜0．05），저우침자조，단차이무통계학의의（P ＞0．05）。광경화전경하 EGC 억제조결장조직형태개변교위엄중。결론전침자격대장유모혈가제고 EGC 세포중 GDNF 단백급기 mRNA 적표체수평，수복수손결장상피세포，진이개선장도전수공능。
ABSTRACTOBJECTIVE To explore the mechanism of electroacupuncture at Back?shu and Front?mu points for functional constipation treatment.METHODS 32 mice were randomly divided into control groupmodel groupelectroacupunctureEA group and EGC group.Compound diphenoxylate were gavaged for modelingand electroacupuncture Tianshu and Dachangshu points30 min each time for a total of 5 times treatment.By light microscopyelectron microscopyimmunohisto-chemistry and in situ hybridizationthe morphology of colonic epithelial structure were observedand the level of GDNF pro-tein and mRNA expression in colon were detected.RESULTS The expression of GDNF protein and mRNA in model group was significantly decreasedP <0.01.Electroacupuncture at back?Shu and front?Mu points improved the expression of GDNF protein and mRNAP <0.05and light microscope and electron microscope showed that acupuncture repaired damaged depi-thelial cellsand then improved the intestinal transmission function.The expression of GDNF protein in EGC group showed no significant difference with the model groupP >0.05but significantly decreasedcompared with EA groupP <0.05.GD-NF mRNA was significantly higher than model groupP <0.05but lower than EA group with no significant differenceP>0.05.The morphology change of colon tissue is more serious.CONCLUSION Electroacupuncture at back?Shu and front? Mu points can improve the expression of GDNF protein and mRNA in EGC cellsrepair the colonic epithelial cellsand then improve the intestinal transmission function.