CAJ | 학술논문

目的：探讨三黄煎剂对乳腺癌 MCF？7、MDA？MB？231细胞凋亡的影响及 Aurora 激酶 A（Aurora A）蛋白表达及功能的影响，并探讨其内在机制。方法采用 CCK？8法检测三黄煎剂对乳腺癌细胞 MCF？7、MDA？MB？231增殖的影响。AnnexinV？ FITC／PI 法检测 MCF？7、MDA？MB？231细胞凋亡率。q？PCR 法检测 MCF？7、MDA？MB？231细胞 Aurora A、p53的 mRNA 表达水平。Western Blot 法检测 MCF？7、MDA？MB？231细胞凋亡相关蛋白的表达及 Aurora A 蛋白的表达。结果三黄煎剂对MCF？7、MDA？MB？231细胞增殖抑制率呈浓度梯度依赖增长（P ＜0．05），给药48 h 疗效好于24 h（P ＜0．05），与给药72 h 无统计学差异（P ＞0．05）。三黄煎剂能够诱导 MCF？7、MDA？MB？231细胞凋亡，并上调 c？PARP、c？Caspase 3、Bax 蛋白的表达，下调 Bcl？2蛋白的表达，呈浓度梯度依赖。三黄煎剂能够下调 Aurora A 蛋白及 mRNA 的表达、上调 p53蛋白及 mRNA 的表达。结论三黄煎剂能够通过下调 Aurora A 蛋白及 mRNA 的表达，抑制 Aurora A 的生物活性，抑制 MCF？7、MDA？MB？231细胞增殖，诱导其凋亡。
목적：탐토삼황전제대유선암 MCF？7、MDA？MB？231세포조망적영향급 Aurora 격매 A（Aurora A）단백표체급공능적영향，병탐토기내재궤제。방법채용 CCK？8법검측삼황전제대유선암세포 MCF？7、MDA？MB？231증식적영향。AnnexinV？ FITC／PI 법검측 MCF？7、MDA？MB？231세포조망솔。q？PCR 법검측 MCF？7、MDA？MB？231세포 Aurora A、p53적 mRNA 표체수평。Western Blot 법검측 MCF？7、MDA？MB？231세포조망상관단백적표체급 Aurora A 단백적표체。결과삼황전제대MCF？7、MDA？MB？231세포증식억제솔정농도제도의뢰증장（P ＜0．05），급약48 h 료효호우24 h（P ＜0．05），여급약72 h 무통계학차이（P ＞0．05）。삼황전제능구유도 MCF？7、MDA？MB？231세포조망，병상조 c？PARP、c？Caspase 3、Bax 단백적표체，하조 Bcl？2단백적표체，정농도제도의뢰。삼황전제능구하조 Aurora A 단백급 mRNA 적표체、상조 p53단백급 mRNA 적표체。결론삼황전제능구통과하조 Aurora A 단백급 mRNA 적표체，억제 Aurora A 적생물활성，억제 MCF？7、MDA？MB？231세포증식，유도기조망。
ABSTRACTOBJECTIVE To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang in-duced apoptosis.METHODS The inhibition of breast cancer cells proliferation was determined by CCK?8 assay.The apoptosis of breast cancer cells was detected by AnnexinV?FITC/PI Staining.The expression of mRNA of Aurora A was examined by q ?PCR analysis.The expression of apoptosis?related proteins and Aurora A were determined by Western Blot analysis.RE-SULTS San Huang decoction inhibited the proliferation of breast cancer cells in a does?dependent mannerP <0.05.The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hoursP <0.05 although similar as 72 hoursP >0.05.San Huang decoction was also found to induce apoptosis in both MCF?7 and MDA?MB?231 cell lines in a dose?dependent manner.Consistent with cellular resultsSan Huang decoction treatment signifi-cantly increased the apoptosis?related protein level of cleaved?PARPc?PARPcleaved?Caspase 3c?Caspase 3and Baxdown ?regulated Bcl?2 in a does?dependent manner.MeanwhileSan Huang decoction decreased the mRNA and protein level of Auro-ra A and increased those of p53 in a does?dependent manner.CONCLUSION San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.