CAJ | 학술논문

研究了铜绿微囊藻藻细胞全生命周期中氮的转化过程以及藻类有机物的性质,结果表明:溶解性有机氮(DON)在藻类生长周期中总体呈现上升趋势,藻细胞在其生长初期释放量小于 0.05mgDON/107cells,衰亡期释放能力约为 0.40mgDON/107cells;胞外有机物(EOM)中分子量分布在对数期和衰亡期以<1kDa 和>100kDa 为主,稳定期以<30kDa 为主;胞内有机物(IOM)的分子量分布随藻的生长变化较小,集中在<1kDa和>100kDa范围内.三维荧光光谱分析表明,EOM中荧光物质主要为类腐植酸和溶解性微生物代谢产物;而IOM主要由类芳香蛋白和溶解性微生物代谢产物组成.
연구료동록미낭조조세포전생명주기중담적전화과정이급조류유궤물적성질,결과표명:용해성유궤담(DON)재조류생장주기중총체정현상승추세,조세포재기생장초기석방량소우 0.05mgDON/107cells,쇠망기석방능력약위 0.40mgDON/107cells;포외유궤물(EOM)중분자량분포재대수기화쇠망기이<1kDa 화>100kDa 위주,은정기이<30kDa 위주;포내유궤물(IOM)적분자량분포수조적생장변화교소,집중재<1kDa화>100kDa범위내.삼유형광광보분석표명,EOM중형광물질주요위류부식산화용해성미생물대사산물;이IOM주요유류방향단백화용해성미생물대사산물조성.
Formation rules of dissolved organic nitrogen (DON), as well as characteristics of extracellular oganic matter (EOM) & intracellular organic matter (IOM) during the growth ofMicrocystis aeruginous have been studied. The results show as follows: DON concentration of EOM shows a growing tendency during the growth ofMicrocystis aeruginous, the yield of which is less than 0.05mg DON per 107cells in the early growth phase, around 0.40mg DON per 107cells in the late growth phase, respectively; molecular weight distributions of EOM during exponential phase and decline phase mainly concentrate in range of less than 1kDa and more than 100kDa, while organic matter less than 30kDa dominating EOM in stationary phase;molecular weight distributions of IOM concentrate in range of more than 1kDa and less than 100kDa in the three phases. The main fluorescence regions of EOM occur at the fluorescence region of humic acid-like materials and soluble microbial products. By contrast, the region of aromatic proteins and soluble microbial products dominate the main region of EEM spectra of IOM.