CAJ | 학술논문

采用溶液培养方法，研究了0．01、0．1、0．3、0．6、1 mmol·L－1等不同浓度外源硫化氢供体硫氢化钠（NaHS ）对干旱条件下玉米幼苗叶片及根系水分生理特性的影响。结果表明，干旱胁迫显著提高了玉米幼苗根系活力、根系和叶片可溶性糖含量、可溶性蛋白含量和脯氨酸含量，显著降低了叶绿素含量及叶片和根系相对含水量。外源0．01 mmol·L－1 NaHS预处理可以显著增加干旱胁迫下根叶相对含水量、脯氨酸含量、叶绿素含量及根系可溶性糖和可溶性蛋白含量，但对叶片可溶性糖和可溶性蛋白含量及根系活力没有显著影响。外源0．1 mmol·L－1和0．3 mmol·L－1 NaHS预处理均可以显著增加干旱胁迫下根叶相对含水量、脯氨酸含量、叶绿素含量、根系可溶性糖和可溶性蛋白含量及根系活力，但对叶片可溶性糖和可溶性蛋白含量没有显著影响。外源0．6 mmol·L－1 NaHS预处理可以显著增加干旱胁迫下根叶相对含水量、叶绿素a含量、叶片可溶性糖含量、根系可溶性蛋白含量及根系活力，但对根系脯氨酸含量、叶片可溶性蛋白和叶绿素b含量没有显著影响，显著降低了根系可溶性糖和脯氨酸含量。外源1 mmol·L－1 NaHS预处理可以显著增加干旱胁迫下根系相对含水量、可溶性蛋白含量及根系活力，但对叶片相对含水量、可溶性蛋白和叶绿素a含量没有显著影响，显著降低了根叶可溶性糖和脯氨酸含量以及叶绿素b含量。这说明，低浓度硫化氢在一定程度上可以增加干旱胁迫下植株的渗透调节物质含量，从而维持自身的水分平衡，提高对干旱环境的适应能力。
채용용액배양방법，연구료0．01、0．1、0．3、0．6、1 mmol·L－1등불동농도외원류화경공체류경화납（NaHS ）대간한조건하옥미유묘협편급근계수분생리특성적영향。결과표명，간한협박현저제고료옥미유묘근계활력、근계화협편가용성당함량、가용성단백함량화포안산함량，현저강저료협록소함량급협편화근계상대함수량。외원0．01 mmol·L－1 NaHS예처리가이현저증가간한협박하근협상대함수량、포안산함량、협록소함량급근계가용성당화가용성단백함량，단대협편가용성당화가용성단백함량급근계활력몰유현저영향。외원0．1 mmol·L－1화0．3 mmol·L－1 NaHS예처리균가이현저증가간한협박하근협상대함수량、포안산함량、협록소함량、근계가용성당화가용성단백함량급근계활력，단대협편가용성당화가용성단백함량몰유현저영향。외원0．6 mmol·L－1 NaHS예처리가이현저증가간한협박하근협상대함수량、협록소a함량、협편가용성당함량、근계가용성단백함량급근계활력，단대근계포안산함량、협편가용성단백화협록소b함량몰유현저영향，현저강저료근계가용성당화포안산함량。외원1 mmol·L－1 NaHS예처리가이현저증가간한협박하근계상대함수량、가용성단백함량급근계활력，단대협편상대함수량、가용성단백화협록소a함량몰유현저영향，현저강저료근협가용성당화포안산함량이급협록소b함량。저설명，저농도류화경재일정정도상가이증가간한협박하식주적삼투조절물질함량，종이유지자신적수분평형，제고대간한배경적괄응능력。
Effects of different concentrations of exogenous hydrogen sulfide were studied on the physiological charac-teristics of maize seedlings under drought conditions .The results showed that pretreatment with 0 .01 mmol·L-1 NaHS could significantly increase relative water content (RWC ) ,proline content in root and leaf ,chlorophyll content and solu-ble sugar and soluble protein content in root ,but this had no significant effects on soluble sugar content and soluble pro-tein content in leaf under drought stress .Pretreatment with exogenous 0 .1 mmol·L-1 and 0 .3 mmol·L-1 NaHS could significantly increase RWC ,proline contents in root and leaf ,chlorophyll content ,soluble protein and soluble sugar con-tent of root and root activity ,but these had no significant impacts on soluble sugar content and soluble protein content in leaf under drought stress .Pretreatment with 0 .6 mmol·L-1 NaHS could significantly increase RWC of root and leaf , chlorophyll A content ,soluble sugar content ,soluble protein contents in leaf and root activity ,but this had no significant effects on proline content ,soluble protein in root and chlorophyll B content .Pretreatment with 0 .6 mmol·L-1 NaHS sig-nificantly reduced soluble sugar and proline contents in root under drought stress .Pretreatment with 1 mmol·L-1 NaHS could significantly increase root RWC ,soluble protein content and root activity ,but this had no significant effects on leaf relative water content ,soluble protein and chlorophyll A content .Pretreatment with 1 mmol·L-1 NaHS could significant-ly reduce soluble sugar and proline content in root and leaf ,and chlorophyll B content under drought stress .Our results suggested that low concentrations of exogenous hydrogen sulfide could maintain the water balance and increase the drought-resistance of plant through increasing the contents of osmoregulation substances .