中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
Chinese Journal of Gastrointestinal Surgery
2015年
9期
941-945
,共5页
吕丽芬%李小飒%刘向强%苏琳娜%李晓华%樊代明
呂麗芬%李小颯%劉嚮彊%囌琳娜%李曉華%樊代明
려려분%리소삽%류향강%소림나%리효화%번대명
胃肿瘤%细胞因子诱导的凋亡抑制因子1%侵袭%迁移
胃腫瘤%細胞因子誘導的凋亡抑製因子1%侵襲%遷移
위종류%세포인자유도적조망억제인자1%침습%천이
Stomach neoplasms%Cytokine-induced apoptosis inhibitor 1%Migration%Invasion
目的：探讨细胞因子诱导的凋亡抑制因子1（CIAPIN1）在胃癌及正常胃黏膜组织中的表达及对胃癌细胞迁移和侵袭能力的影响。方法应用免疫组织化学方法检测CIAPIN1蛋白在15例正常胃黏膜组织、148例胃癌组织（42非转移性胃癌组织、106例转移性胃癌组织）及37例转移性淋巴结组织中的表达。建立高表达和低表达CIAPIN1的胃癌SGC-7901细胞系，通过Transwell实验比较CIAPIN1不同表达水平细胞的迁移和侵袭能力。结果 CIAPIN1在胃癌组织中的表达阳性率显著低于正常胃黏膜组织（41．2％比86．7％，P＝0．0008）；在转移性胃癌组织中的表达阳性率明显低于非转移性胃癌组织（34．9％比71．4％，P＝0．0000）；在TNM分期较早组织中表达的阳性率明显高于分期较晚者（Ⅰ期、Ⅱ期和Ⅲ期分别为55．0％、53．5％和24．4％，P＝0．0037）；而转移性胃癌组织和转移性淋巴结组织中CIAPIN1表达阳性率的差异无统计学意义（34．9％比21．6％，P＝0．3700）。Transwell实验结果显示，与普通胃癌SGC-7901细胞相比，高表达CIAPIN1可显著抑制细胞迁移和侵袭，而低表达CIAPIN1可显著促进细胞的迁移和侵袭（均P＜0．05）。结论 CIAPIN1在胃癌组织中表达阳性率低，可抑制胃癌的迁移和侵袭。
目的：探討細胞因子誘導的凋亡抑製因子1（CIAPIN1）在胃癌及正常胃黏膜組織中的錶達及對胃癌細胞遷移和侵襲能力的影響。方法應用免疫組織化學方法檢測CIAPIN1蛋白在15例正常胃黏膜組織、148例胃癌組織（42非轉移性胃癌組織、106例轉移性胃癌組織）及37例轉移性淋巴結組織中的錶達。建立高錶達和低錶達CIAPIN1的胃癌SGC-7901細胞繫，通過Transwell實驗比較CIAPIN1不同錶達水平細胞的遷移和侵襲能力。結果 CIAPIN1在胃癌組織中的錶達暘性率顯著低于正常胃黏膜組織（41．2％比86．7％，P＝0．0008）；在轉移性胃癌組織中的錶達暘性率明顯低于非轉移性胃癌組織（34．9％比71．4％，P＝0．0000）；在TNM分期較早組織中錶達的暘性率明顯高于分期較晚者（Ⅰ期、Ⅱ期和Ⅲ期分彆為55．0％、53．5％和24．4％，P＝0．0037）；而轉移性胃癌組織和轉移性淋巴結組織中CIAPIN1錶達暘性率的差異無統計學意義（34．9％比21．6％，P＝0．3700）。Transwell實驗結果顯示，與普通胃癌SGC-7901細胞相比，高錶達CIAPIN1可顯著抑製細胞遷移和侵襲，而低錶達CIAPIN1可顯著促進細胞的遷移和侵襲（均P＜0．05）。結論 CIAPIN1在胃癌組織中錶達暘性率低，可抑製胃癌的遷移和侵襲。
목적：탐토세포인자유도적조망억제인자1（CIAPIN1）재위암급정상위점막조직중적표체급대위암세포천이화침습능력적영향。방법응용면역조직화학방법검측CIAPIN1단백재15례정상위점막조직、148례위암조직（42비전이성위암조직、106례전이성위암조직）급37례전이성림파결조직중적표체。건립고표체화저표체CIAPIN1적위암SGC-7901세포계，통과Transwell실험비교CIAPIN1불동표체수평세포적천이화침습능력。결과 CIAPIN1재위암조직중적표체양성솔현저저우정상위점막조직（41．2％비86．7％，P＝0．0008）；재전이성위암조직중적표체양성솔명현저우비전이성위암조직（34．9％비71．4％，P＝0．0000）；재TNM분기교조조직중표체적양성솔명현고우분기교만자（Ⅰ기、Ⅱ기화Ⅲ기분별위55．0％、53．5％화24．4％，P＝0．0037）；이전이성위암조직화전이성림파결조직중CIAPIN1표체양성솔적차이무통계학의의（34．9％비21．6％，P＝0．3700）。Transwell실험결과현시，여보통위암SGC-7901세포상비，고표체CIAPIN1가현저억제세포천이화침습，이저표체CIAPIN1가현저촉진세포적천이화침습（균P＜0．05）。결론 CIAPIN1재위암조직중표체양성솔저，가억제위암적천이화침습。
[Abatract] Objective To examine the expression of cytokine-induced apoptosis inhibitor1 (CIAPIN1) in gastric cancer and normal mucosa tissues, and to investigate its effects on migration and invasion of gastric cancer cells. Methods Immunohistochemistry was used to detect CIAPIN1 expression in 15 samples of normal gastric mucosa tissues , 148 samples of gastric cancer tissues (42 of non-metastasis gastric cancer tissues without other organs or lymph node metastasis , 106 of metastasis gastric cancer tissues with lymph node metastasis), and 37 samples of gastric cancer lymph node metastasis tissues. Association of CIAPIN1 expression with clinicopathological characteristics of gastric cancer was analyzed by Chi-square test. SGC-7901 cell lines of high CIAPIN1 expression and low CIAPIN1 expression were established. Transwell assay was applied to detect the invasion and migration of CIAPIN1-regulated gastric cancer cells. Results Positive rate of CIAPIN1 expression in gastric cancer tissues was lower than that in normal gastric mucosa tissues (41.2% vs. 86.7%, P=0.0008), and in metastasis gastric cancer tissues was also lower than that in non-metastasis gastric cancer tissues (34.9% vs. 71.4%, P=0.0000). Furthermore, the positive rate of CIAPIN1 expression was closely related to the TNM staging of gastric cancer (TNM stage Ⅰ,ⅡandⅢwere 55.0%, 53.5%and 24.4%, respectively, P=0.0037). But there was no significant difference of positive expressive rate between metastatic gastric cancer tissues and metastatic lymph node tissues (34.9% vs. 21.6%, P=0.3700). Transwell assay showed that up-regulated CIAPIN1 expression would significantly reduce , while down-regulated CIAPIN1 expression would significantly promote the invasion and migration of SGC-7901 cells (all P<0.05). Conclusion Positive rate of CIAPIN1 expression is low in gastric cancer tissues and shows inhibition of invasion and migration of gastric cancer cells.
