CAJ | 학술논문

目的：探讨微丝附着梁蛋白（ Girdin）在大鼠自体移植静脉中的表达变化及其与内膜增生的关系。方法28只雄性SD大鼠随机均分为4组，建立颈外静脉移植至颈总动脉模型，于术后3 d、7 d、14 d及28 d采集移植静脉，同时取对侧静脉作对照。光镜下观察血管内膜，应用图像软件计算新生内膜增生厚度，免疫组化及Western Blot法检测Girdin及增殖细胞核抗原（ PCNA）蛋白在静脉桥及对侧静脉血管壁的表达变化情况。结果移植术后，移植静脉内膜术后7 d增厚明显（P＜0．01），14 d 时增厚更为明显，28 d 时达到高峰；免疫组化结果显示 Girdin 和 PCNA 蛋白均在术后3 d 明显增加（P＜0．01），前者于14 d出现高峰，后者于7 d达高峰，而后逐渐下降；术后 Girdin 蛋白表达与 PCNA 蛋白表达呈正相关（ r＝0．938，P＜0．01）。对侧静脉有少量Girdin蛋白表达，而几乎无 PCNA蛋白表达。结论静脉桥术后Girdin蛋白的表达增高，并与静脉桥平滑肌细胞的增殖密切相关，这可能在静脉桥术后内膜增生中起重要作用。
목적：탐토미사부착량단백（ Girdin）재대서자체이식정맥중적표체변화급기여내막증생적관계。방법28지웅성SD대서수궤균분위4조，건립경외정맥이식지경총동맥모형，우술후3 d、7 d、14 d급28 d채집이식정맥，동시취대측정맥작대조。광경하관찰혈관내막，응용도상연건계산신생내막증생후도，면역조화급Western Blot법검측Girdin급증식세포핵항원（ PCNA）단백재정맥교급대측정맥혈관벽적표체변화정황。결과이식술후，이식정맥내막술후7 d증후명현（P＜0．01），14 d 시증후경위명현，28 d 시체도고봉；면역조화결과현시 Girdin 화 PCNA 단백균재술후3 d 명현증가（P＜0．01），전자우14 d출현고봉，후자우7 d체고봉，이후축점하강；술후 Girdin 단백표체여 PCNA 단백표체정정상관（ r＝0．938，P＜0．01）。대측정맥유소량Girdin단백표체，이궤호무 PCNA단백표체。결론정맥교술후Girdin단백적표체증고，병여정맥교평활기세포적증식밀절상관，저가능재정맥교술후내막증생중기중요작용。
Objective To explore the expression change of girders of actin filaments( Girdin) in autologous vein graft of rat and its relationship with intimal hyperplasia.Methods A total of 28 male Sprague-Dawley(SD) rats were randomly divided into four groups,and the external jugular vein-to-common carotid artery transplant model was established.The vein graft samples were harvested 3,7,14 and 28 days after operation,respectively,and contralateral veins were collected as control.Intima was observed under the optical microscope and neointima thickness was calculated using the computer image analysis system.The expression changes of Girdin and proliferating cell nuclear antigen( PCNA) in the vessel wall of grafted veins and contralateral veins were detected by both immunohistochemistry and Western Blot.Results In the grafted veins,intima was significantly thicker on the 7th day after operation (P<0.01),and this change was more significant on the 14th day,then culminated on the 28th day.The result of immunohistochemistry showed that both Girdin and PCNA expressions increased obviously on the third day after operation (P<0.01),peaked on the 14th(Girdin) or 7th(PCNA) day,and then decreased gradually after that.A positive correlation existed between Girdin and PCNA expressions postoperatively(r=0.938,P<0.01).A little expression of Girdin and little expression of PCNA were found in the contralateral veins.Conclusion Girdin expression increases after vein grafting,and is closely related to the proliferation of media smooth muscle cells in grafted veins,which might play an important role in the process of intimal hyperplasia after vein grafting.