CAJ | 학술논문

针对大鼠气道平滑肌细胞激光扫描共聚焦显微图像，从细胞切面角度提出一种细胞内部微丝纤维形态与分布特征的定量分析方法。利用形态学与阈值分割法对细胞中微丝纤维主干进行提取，并对提取后的微丝纤维进行了数量、长度、宽度等形态特征计算，同时利用灰度共生矩阵分析微丝的分布特征。实验结果表明，所给出的定量特征提取方法能够表达大鼠气道平滑肌细胞切面内微丝纤维的几何特征和分布状态。并通过模拟微丝纤维变化，发现能量、熵能够较好地描述微丝的响应情况。
침대대서기도평활기세포격광소묘공취초현미도상，종세포절면각도제출일충세포내부미사섬유형태여분포특정적정량분석방법。이용형태학여역치분할법대세포중미사섬유주간진행제취，병대제취후적미사섬유진행료수량、장도、관도등형태특정계산，동시이용회도공생구진분석미사적분포특정。실험결과표명，소급출적정량특정제취방법능구표체대서기도평활기세포절면내미사섬유적궤하특정화분포상태。병통과모의미사섬유변화，발현능량、적능구교호지묘술미사적향응정황。
Based on laser scanning confocal microscopy imaging of airway smooth muscle cells,we presented a new method to quantify and analyze the shape,orientation and their distributions of the intracellular microfilaments from the view of the cell sections. The main structure of the microfilaments were extracted by morphology and threshold segmentation method,and then quantitatively ana-lyzed by shape and orientation distribution features including number,length,width,and analyzed texture features with gray level co -occurrence matrix.The results show that the method with quantitative feature extraction can express the shape and distribution of mi-crofilaments in rat airway smooth muscle cells.Through simulated changes of actin filaments,the parameters as energy,entropy can well describe the response of filaments.