CAJ | 학술논문

目的：调查脂联素基因多态性位点与结直肠癌发病风险的关联性。方法采用1∶1病例对照研究,对250例病例(病例组)及匹配对照(对照组,n=250)抽取静脉血,提取基因组 DNA 后,运用 Taqman 荧光定量 PCR 方法,对研究对象3个基因多态性位点(rs266729、rs2241766及 rs1501299)进行检测。Logistic 回归分析用于检测基因型别、基因-基因、基因-环境交互作用。结果对 rs266729,病例组携带 GG 及 CG+GG 基因型别均可引起结直肠癌发病风险增高[OR (95%CI )：1.87(1.01~3.47)、1.63(1.14~2.32)]。对 rs2441766,病例组携带 TG+GG 基因型别可引起结直肠癌发病风险增高[OR (95%CI )：1.45(1.02~2.06)]。对 rs1501299,病例组携带 GT+GG 基因型别的发病风险是对照组的0.61倍(95%CI ：0.43~0.88)。Logistic 回归分析表明,rs266729基因多态性与体质量指数(BMI)存在交互作用,两者使得病例组发生结直肠癌的风险是对照组的1.16倍(95%CI ：1.03~1.30)。结论脂联素基因多态性可引起结直肠癌的发病风险增高,rs266729基因多态性与 BMI 共同影响结直肠癌发病风险。
목적：조사지련소기인다태성위점여결직장암발병풍험적관련성。방법채용1∶1병례대조연구,대250례병례(병례조)급필배대조(대조조,n=250)추취정맥혈,제취기인조 DNA 후,운용 Taqman 형광정량 PCR 방법,대연구대상3개기인다태성위점(rs266729、rs2241766급 rs1501299)진행검측。Logistic 회귀분석용우검측기인형별、기인-기인、기인-배경교호작용。결과대 rs266729,병례조휴대 GG 급 CG+GG 기인형별균가인기결직장암발병풍험증고[OR (95%CI )：1.87(1.01~3.47)、1.63(1.14~2.32)]。대 rs2441766,병례조휴대 TG+GG 기인형별가인기결직장암발병풍험증고[OR (95%CI )：1.45(1.02~2.06)]。대 rs1501299,병례조휴대 GT+GG 기인형별적발병풍험시대조조적0.61배(95%CI ：0.43~0.88)。Logistic 회귀분석표명,rs266729기인다태성여체질량지수(BMI)존재교호작용,량자사득병례조발생결직장암적풍험시대조조적1.16배(95%CI ：1.03~1.30)。결론지련소기인다태성가인기결직장암적발병풍험증고,rs266729기인다태성여 BMI 공동영향결직장암발병풍험。
Objective To investigate the adiponectin(ADIPOQ)gene polymorphisms and which association with colorectal cancer(CRC).Methods Genotyping of blood samples were performed for 250 case-control pairs.Taqman real-time PCR was used to test the three single nucleotide polymorphisms(SNPs),namely rs266729,rs2441 766,rs1 501299.Logistic regression was applied to assess the effects of three SNPs,the gene-gene,and gene-environment interactions on CRC risk.Results The ADIPOQ rs266729 GG and CG+GG genotype had a higher CRC risk than those carrying the CC genotype[OR (95%CI ):1.87 (1.01 - 3.47),1.63 (1.14-2.32),respectively].The same results was observed in cases who carried TG+GG genotype vs .TT genotype in rs2441 766 [OR(95%CI ):1.45(1.02-2.06)].The rs1 501299 GT+TT genotype had a lower CRC risk than those carrying the GG genotype [OR(95%CI ):0.61(0.43-0.88)].Furthermore,in two-factor gene-environment interaction analyses,rs266729 presented signifi-cant interactions with Body mass index (BMI),with OR of 1.1 6 (95%CI :1.03-1.30).Conclusion The results suggest that vari-ants in ADIPOQ may contribute to increased colorectal cancer risk and this contribution may be modified by BMI.