CAJ | 학술논문

目的：研究藤茶中提取的二氢杨梅素(DMY)对胃癌细胞 SGC7901生长的影响,以筛选高效低毒的抗肿瘤药物。方法使用超声中性水浸提法提取藤茶中的活性成分 DMY,反相高效液相色谱(RP-HPLC)对提取物进行分离纯化,纯度达99%以上,CCK-8比色法检测纯化后的 DMY 对肿瘤细胞存活率的影响；流式细胞术检测 DMY 促进细胞凋亡的能力。结果直接从野生藤茶中提取 DMY,经过 RP-HPLC 分离纯化后的 DMY 的纯度达到99%,DMY 在低浓度时(≤1 mmol)对肿瘤细胞具有显著的杀伤效应(P <0.05),且其抑制率与药物浓度呈剂量-效应关系；同时 DMY 具有促进细胞凋亡的能力,且其促凋亡作用随着浓度的增加而增强。结论藤茶中提取的 DMY 具有显著杀伤胃癌细胞 SGC7901的效应,将来可能成为治疗肿瘤的一类药物。
목적：연구등다중제취적이경양매소(DMY)대위암세포 SGC7901생장적영향,이사선고효저독적항종류약물。방법사용초성중성수침제법제취등다중적활성성분 DMY,반상고효액상색보(RP-HPLC)대제취물진행분리순화,순도체99%이상,CCK-8비색법검측순화후적 DMY 대종류세포존활솔적영향；류식세포술검측 DMY 촉진세포조망적능력。결과직접종야생등다중제취 DMY,경과 RP-HPLC 분리순화후적 DMY 적순도체도99%,DMY 재저농도시(≤1 mmol)대종류세포구유현저적살상효응(P <0.05),차기억제솔여약물농도정제량-효응관계；동시 DMY 구유촉진세포조망적능력,차기촉조망작용수착농도적증가이증강。결론등다중제취적 DMY 구유현저살상위암세포 SGC7901적효응,장래가능성위치료종류적일류약물。
Objective To investigate the anticancer efficacy of dihydromyricetin (DMY)in gastric cancer cells SGC7901. Methods The DMY was puritied using RP-HPLC was almost 98%.The cell viability was assessed by CCK-8 Assay.The cell ap-optosis was assessed by flow cytometry.Using ultrasonic extraction with neutral water and reversed-phase high-performance liquid chromatography,DMY was purified from rattan tea.Results By using RP-HPLC,the purified DMY is almost 99%.DMY inhibi-ted the growth of tumor cells in the low concentration(≤ 1 mmol)with a dose-dependent manner(P <0.05 ).Further study on DMY showed that it induced apoptosis in SGC7901 cell line with a dose-dependent manner.Conclusion DMY is a potential thera-peutic agent for gastric cancer.