CAJ | 학술논문

目的：研究α-硫辛酸(α-LA)对糖尿病大鼠视网膜病变及血管内皮生长因子(VEGF)表达的影响及机制。方法选择72只健康的雄性 Wistar 大鼠作为实验材料,将72只大鼠分为4组：空白对照组(A 组)12只,糖尿病模型组(B 组)24只,α-LA治疗组(C 组)24只,葡萄糖干预组(D 组)12只,B~D 组大鼠按照60 mg/kg 的剂量腹腔注射链脲佐菌素(STZ),C 组用100 mg/kg 的α-LA 灌胃,D 组用5.0%的葡萄糖溶液灌胃。比较各组大鼠的体质量、空腹血糖(FPG)、空腹胰岛素(FINS),稳态胰岛素抵抗指数(HOMA-IR)、VEGF 表达、超氧化钠歧化酶(SOD)、谷胱甘肽(GSH)和 IL-6水平,比较 B~D 组大鼠的 GR 分期。结果72 h 后,B~D 组大鼠模型构建成功,A 组大鼠 FPG 为(4.57±0.15)mmol/L,B 组为(21.72±4.28)mmol/L,C 组为(21.54±4.96)mmol/L,D 组为(21.83±4.77)mmol/L,A 组与 B、C 组比较差异有统计学意义(P <0.05)；A 组大鼠体质量(210.5±5.2) g,B 组(211.2±5.7)g,C 组(209.8±5.8)g,D 组(208.7±3.4)g,4组的差异无统计学意义(P >0.05)。4组大鼠4、8、12周的体质量、FPG、FINS、HOMA-IR、VEGF 表达、SOD、GSH 和 IL-6水平差异有统计学意义(P <0.05)。12周后,B 组、C 组和 D 组大鼠的眼底镜检查 GR 分期差异有统计学差异(P <0.05)。结论α-LA 的应用可以有效抑制大鼠视网膜 VEGF 的表达,这一作用可能与改善胰岛素抵抗、抑制慢性炎症和氧化应激多个环节相关。
목적：연구α-류신산(α-LA)대당뇨병대서시망막병변급혈관내피생장인자(VEGF)표체적영향급궤제。방법선택72지건강적웅성 Wistar 대서작위실험재료,장72지대서분위4조：공백대조조(A 조)12지,당뇨병모형조(B 조)24지,α-LA치료조(C 조)24지,포도당간예조(D 조)12지,B~D 조대서안조60 mg/kg 적제량복강주사련뇨좌균소(STZ),C 조용100 mg/kg 적α-LA 관위,D 조용5.0%적포도당용액관위。비교각조대서적체질량、공복혈당(FPG)、공복이도소(FINS),은태이도소저항지수(HOMA-IR)、VEGF 표체、초양화납기화매(SOD)、곡광감태(GSH)화 IL-6수평,비교 B~D 조대서적 GR 분기。결과72 h 후,B~D 조대서모형구건성공,A 조대서 FPG 위(4.57±0.15)mmol/L,B 조위(21.72±4.28)mmol/L,C 조위(21.54±4.96)mmol/L,D 조위(21.83±4.77)mmol/L,A 조여 B、C 조비교차이유통계학의의(P <0.05)；A 조대서체질량(210.5±5.2) g,B 조(211.2±5.7)g,C 조(209.8±5.8)g,D 조(208.7±3.4)g,4조적차이무통계학의의(P >0.05)。4조대서4、8、12주적체질량、FPG、FINS、HOMA-IR、VEGF 표체、SOD、GSH 화 IL-6수평차이유통계학의의(P <0.05)。12주후,B 조、C 조화 D 조대서적안저경검사 GR 분기차이유통계학차이(P <0.05)。결론α-LA 적응용가이유효억제대서시망막 VEGF 적표체,저일작용가능여개선이도소저항、억제만성염증화양화응격다개배절상관。
Objective To study the effect of α-lipoic acid on the retinal expression level of VEGF and diabetic retinopathy in rats with diabetes mellitus and mechanism.Methods Totally 72 Wistar rats were divided into 4 groups:12 (control group)in group A,24 in modeling group(group B),24 in group treated withα-LA(group C)and 12 in high-glucose(group D).Group B to group D were given 60 mg/kg STZ through intraperitoneal injection,rats in group C were given 100 mg/kg α-LA and rats in group D were given 5.0% glucose-solution.The body mass,FPG,FINS,HOMA-IR,expression level of VEGF,activity of SOD,GSH and IL-6 of 4 groups were compared by statistics.Results After 72 h,the FPG of group A was(4.57 ±0.1 5 )mmol/L,that of group B was (21.72±4.28)mmol/L,that of group C was(21.54±4.96)mmol/L and that of group D was(21.83±4.77)mmol/L,the difference had statistical significance (P <0.05).The body mass of group A was(210.5±5.2)g,that of group B was(21 1.2 ±5.7)g,that of group C was(209.8±5.8)g and that of group D was(208.7±3.4)g,the difference had no statistical significance (P >0.05).The body mass,FPG,FINS,HOMA-IR,expression level of VEGF,activity of SOD,GSH and IL-6 among 4 groups at 4 w,8 w and 12 w had statistical difference (P <0.05).After 12 w,the difference of GR stage among group B to group D had statistical significance (P <0.05).Conclusion α-LA can inhibit the expression of VEGF in rats with diabetes mellitus,which is related to its ability to re-duce the oxidative stress and inflammation reaction,as well as to alleviate the insulin resistance.