国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
International Journal of Traditional Chinese Medicine
2015年
10期
924-927
,共4页
马兜铃酸%九味羌活丸%限量测定
馬兜鈴痠%九味羌活汍%限量測定
마두령산%구미강활환%한량측정
Aristolochic acid%Jiu Wei Qiang Huo Wan%Limitation
目的 建立九味羌活丸中马兜铃酸A的限量检测方法.方法 九味羌活丸样品经固相萃取小柱处理,采用高效液相色谱法,色谱柱为Phenomenex Luna C18柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸溶液为流动相,采用梯度洗脱,流速1.0 ml/min,柱温35℃,检测波长250 nm.结果 马兜铃酸A在0.014~0.280μg/ml浓度范围内有良好线性关系(r=0.999 8),检测限(S/N=3)为1.5 ng/ml,定量限(S/N=10)为4.5 ng/ml平均加样回收率为96.3%.结论 本方法灵敏度高、准确、重复性好、专属性强,可用于九味羌活丸中马兜铃酸A的限量检测.
目的 建立九味羌活汍中馬兜鈴痠A的限量檢測方法.方法 九味羌活汍樣品經固相萃取小柱處理,採用高效液相色譜法,色譜柱為Phenomenex Luna C18柱(4.6 mm×250 mm,5μm),以乙腈-0.05%燐痠溶液為流動相,採用梯度洗脫,流速1.0 ml/min,柱溫35℃,檢測波長250 nm.結果 馬兜鈴痠A在0.014~0.280μg/ml濃度範圍內有良好線性關繫(r=0.999 8),檢測限(S/N=3)為1.5 ng/ml,定量限(S/N=10)為4.5 ng/ml平均加樣迴收率為96.3%.結論 本方法靈敏度高、準確、重複性好、專屬性彊,可用于九味羌活汍中馬兜鈴痠A的限量檢測.
목적 건립구미강활환중마두령산A적한량검측방법.방법 구미강활환양품경고상췌취소주처리,채용고효액상색보법,색보주위Phenomenex Luna C18주(4.6 mm×250 mm,5μm),이을정-0.05%린산용액위류동상,채용제도세탈,류속1.0 ml/min,주온35℃,검측파장250 nm.결과 마두령산A재0.014~0.280μg/ml농도범위내유량호선성관계(r=0.999 8),검측한(S/N=3)위1.5 ng/ml,정량한(S/N=10)위4.5 ng/ml평균가양회수솔위96.3%.결론 본방법령민도고、준학、중복성호、전속성강,가용우구미강활환중마두령산A적한량검측.
Objective To establish a method for the determination of aristolochic acid A in Jiuwei-Qianghuo pill. Method The HPLC system consisted of the Phenomenex Luna C18(4.6 mm × 250 mm, 5μm)column, the mobile phase consisted of acetonitrile and 0.05% H3PO4, gradient elution flow rate was 1.0 ml/min, the column temperature was 35℃, the UV detector was set at 250 nm.Results The linear response range was 0.014-0.280μg/ml(r=0.999 9). The detection limit and quantitation limit of aristolochic acid A in Jiuwei-Qianghuo pill was 1.5 and 4.5 ng/ml. The average recovery of aristolochic acid A was 96.3%. Conclusions The method was high sensitivity, accurate, repeatable and high secificity. It can be used as an inspection method for safe use of Jiuwei-Qianghuo pill.
