国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
International Journal of Traditional Chinese Medicine
2015年
10期
914-919
,共6页
赵靖雯%王敏%卢传坚%王志仁%朱伟
趙靖雯%王敏%盧傳堅%王誌仁%硃偉
조정문%왕민%로전견%왕지인%주위
麦冬%多糖类%响应面分析%提取法
麥鼕%多糖類%響應麵分析%提取法
맥동%다당류%향응면분석%제취법
Ophiopogon japonicus%Polysaccharides%Response surface methodology%Extraction
目的 利用响应面分析法对麦冬多糖的提取工艺进行优化.方法 根据 Box-Behnken 中心组合设计原理,采用响应面分析法优化提取温度、提取时间、提取次数及料液比 4 个因素对麦冬多糖得率的影响,利用Design-Expert 8.05b软件对试验数据进行回归分析.结果 麦冬多糖的最佳工艺条件为提取温度93℃,提取时间2 h,提取次数3次,料液比1:15 g/ml.结论 在此条件下,麦冬多糖的提取率为5.440%,与理论预测值的相对误差为7.328%.
目的 利用響應麵分析法對麥鼕多糖的提取工藝進行優化.方法 根據 Box-Behnken 中心組閤設計原理,採用響應麵分析法優化提取溫度、提取時間、提取次數及料液比 4 箇因素對麥鼕多糖得率的影響,利用Design-Expert 8.05b軟件對試驗數據進行迴歸分析.結果 麥鼕多糖的最佳工藝條件為提取溫度93℃,提取時間2 h,提取次數3次,料液比1:15 g/ml.結論 在此條件下,麥鼕多糖的提取率為5.440%,與理論預測值的相對誤差為7.328%.
목적 이용향응면분석법대맥동다당적제취공예진행우화.방법 근거 Box-Behnken 중심조합설계원리,채용향응면분석법우화제취온도、제취시간、제취차수급료액비 4 개인소대맥동다당득솔적영향,이용Design-Expert 8.05b연건대시험수거진행회귀분석.결과 맥동다당적최가공예조건위제취온도93℃,제취시간2 h,제취차수3차,료액비1:15 g/ml.결론 재차조건하,맥동다당적제취솔위5.440%,여이론예측치적상대오차위7.328%.
Objective To optimize the extraction of polysaccharides from Ophiopogon japonicus by the use of response surface methodology.Method According to the design principle of Box-Behnken, effects of extraction temperature, extraction time , extraction times and solid-liquid ratio on the extraction yield of Ophiopogon japonicus polysaccharides were studied by response surface methodology,meanwhile regression analysis of these experimental data was used by Design-Expert 8.05b software.Results The optimum extraction conditions from Ophiopogon japonicus were concluded as follows: the extraction temperature was 93℃, extraction time was 2 h, extraction times was 3 times, and solid-liquid ratio was 1:15 g/ml.Conclusion Under these conditions, the extraction yield of Ophiopogon japonicus polysaccharides is up to 5.440%, and the relative error is 7.328%.