中国现代医药杂志
中國現代醫藥雜誌
중국현대의약잡지
Modern Medicine Journal of China
2015年
9期
4-8
,共5页
谢秀芳%李多%熊彬%湛晓勤%鄢洁%傅玉琼%唐瑜健%黄越
謝秀芳%李多%熊彬%湛曉勤%鄢潔%傅玉瓊%唐瑜健%黃越
사수방%리다%웅빈%담효근%언길%부옥경%당유건%황월
生长素释放肽%脓毒症%TNF-α%IL-6%炎症反应
生長素釋放肽%膿毒癥%TNF-α%IL-6%炎癥反應
생장소석방태%농독증%TNF-α%IL-6%염증반응
Ghrelin%Sepsis%TNF-α%IL-6%Inflammatory response
目的:探讨生长素释放肽(ghrelin)在脓毒症小鼠肺脏炎症反应中的作用机制。方法①通过腹腔注射内毒素(LPS)建立脓毒症模型,采用腹腔注射外源性ghrelin 200μg/kg进行干预。选取雌性昆明小鼠54只,随机分为对照组、模型组及ghrelin干预组,每组18只,对照组经腹腔注射等量生理盐水;模型组经腹腔内注射脂多糖6mg/kg;干预组先经腹腔注射入外源性ghrelin 200μg/kg,30min后再经腹腔注射脂多糖6mg/kg;②各组分别于3h、9h及18h随机处死6只小鼠,留取肺组织标本行HE染色,观察肺组织病理变化;ELISA法测定炎症因子TNF-α、IL-6的表达量;③统计分析使用SPSS 17.0软件,所得数据以x±s表示,用单因素方差分析来比较组之间总体差异,用SNK方法比较每两组的差异。结果与对照组相比,模型组及干预组中肺组织病理炎症反应加重,相关炎症因子TNF-α、IL-6的表达水平在各时间点均明显升高(P均<0.05),差异有统计学意义;与模型组相比,干预组中肺组织病理炎症反应减轻,相关炎症因子TNF-α、IL-6的表达水平在各时间点均明显降低(P均<0.05),差异有统计学意义。结论外源性ghrelin可通过降低早期炎症因子TNF-α、IL-6的表达,从而减轻脓毒症小鼠肺脏炎症反应,在脓毒症肺脏炎症反应中发挥保护作用。
目的:探討生長素釋放肽(ghrelin)在膿毒癥小鼠肺髒炎癥反應中的作用機製。方法①通過腹腔註射內毒素(LPS)建立膿毒癥模型,採用腹腔註射外源性ghrelin 200μg/kg進行榦預。選取雌性昆明小鼠54隻,隨機分為對照組、模型組及ghrelin榦預組,每組18隻,對照組經腹腔註射等量生理鹽水;模型組經腹腔內註射脂多糖6mg/kg;榦預組先經腹腔註射入外源性ghrelin 200μg/kg,30min後再經腹腔註射脂多糖6mg/kg;②各組分彆于3h、9h及18h隨機處死6隻小鼠,留取肺組織標本行HE染色,觀察肺組織病理變化;ELISA法測定炎癥因子TNF-α、IL-6的錶達量;③統計分析使用SPSS 17.0軟件,所得數據以x±s錶示,用單因素方差分析來比較組之間總體差異,用SNK方法比較每兩組的差異。結果與對照組相比,模型組及榦預組中肺組織病理炎癥反應加重,相關炎癥因子TNF-α、IL-6的錶達水平在各時間點均明顯升高(P均<0.05),差異有統計學意義;與模型組相比,榦預組中肺組織病理炎癥反應減輕,相關炎癥因子TNF-α、IL-6的錶達水平在各時間點均明顯降低(P均<0.05),差異有統計學意義。結論外源性ghrelin可通過降低早期炎癥因子TNF-α、IL-6的錶達,從而減輕膿毒癥小鼠肺髒炎癥反應,在膿毒癥肺髒炎癥反應中髮揮保護作用。
목적:탐토생장소석방태(ghrelin)재농독증소서폐장염증반응중적작용궤제。방법①통과복강주사내독소(LPS)건립농독증모형,채용복강주사외원성ghrelin 200μg/kg진행간예。선취자성곤명소서54지,수궤분위대조조、모형조급ghrelin간예조,매조18지,대조조경복강주사등량생리염수;모형조경복강내주사지다당6mg/kg;간예조선경복강주사입외원성ghrelin 200μg/kg,30min후재경복강주사지다당6mg/kg;②각조분별우3h、9h급18h수궤처사6지소서,류취폐조직표본행HE염색,관찰폐조직병리변화;ELISA법측정염증인자TNF-α、IL-6적표체량;③통계분석사용SPSS 17.0연건,소득수거이x±s표시,용단인소방차분석래비교조지간총체차이,용SNK방법비교매량조적차이。결과여대조조상비,모형조급간예조중폐조직병리염증반응가중,상관염증인자TNF-α、IL-6적표체수평재각시간점균명현승고(P균<0.05),차이유통계학의의;여모형조상비,간예조중폐조직병리염증반응감경,상관염증인자TNF-α、IL-6적표체수평재각시간점균명현강저(P균<0.05),차이유통계학의의。결론외원성ghrelin가통과강저조기염증인자TNF-α、IL-6적표체,종이감경농독증소서폐장염증반응,재농독증폐장염증반응중발휘보호작용。
Objective To discuss the regulating role of ghrelin in lung inflammatory response of mice with sepsis. Methods ①Set up sepsis model by injecting endotoxin into enterocoelia of mice, while injecting ghrelin (200μg/kg) into en-terocoelia of mice as intervention treatment , intraperitoneal injection equivalent amount of saline as a control was injected into enterocoelia of mice. 54 female Kunming mice were randomly divided into control group , model group and intervention group with ghrelin, each group contained 18 mice. Saline was injected into abdominal cavity of mice in control group. Model group with treated lipopolysaccharide 6mg/kg was injected into abdominal cavity. Intervention group: injected the ghrelin 200μg/kg into abdominal cavity, then injected lipopolysaccharide 6mg/kg into the abdominal cavity of mice after 30min. ②Six mice of each group were executed randomly respectively in 3h, 9h and 18h, collected specimen to corresponding test. Took each experi-mental group organization of the right upper lobe for HE staining , then observed the lung tissue pathology. Used enzyme linked immunosorbent (ELISA) to determinate the expression level of TNF alpha, IL-6.③The software of SPSS 17.0 was applicated for statistical analysis of data which was expressed with x±s. Comparison between groups was used by single factor analysis of vari-ance. Results Compared with the control group, lung tissue inflammation of model group and intervention group aggravated, the expression levels of TNF alpha, IL-6 at every time points were significantly higher (P<0.05), the difference had statistical significance. Compared with model group, lung tissue inflammation in the intervention group alleviated, the expression levels of TNF alpha, IL-6 at every time points were significantly lower (P<0.05), the difference had statistical significance. Conclusion Exogenous ghrelin can alleviate lung inflammatory response of mice with sepsis through decreasing the expression of TNF alpha and IL-6, it plays a protective role in sepsis lung inflammation.