国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
International Journal of Traditional Chinese Medicine
2015年
10期
900-903
,共4页
急性脊髓损伤%珍宝丸%丙二醛%神经细胞凋亡率
急性脊髓損傷%珍寶汍%丙二醛%神經細胞凋亡率
급성척수손상%진보환%병이철%신경세포조망솔
Acute spinal cord injury%Zhenbao pill%MDA%Cell apoptosis rate
目的 探讨蒙药珍宝丸对急性脊髓损伤(acute spinal cord injury, ASCI)大鼠的神经保护作用及其机制.方法 按随机数字表法将64只Wistar大鼠分为模型组、珍宝丸组各32只.采用改良Allen's重物打击法制作胸T10水平急性脊髓不完全损伤大鼠模型.造模后30 min,珍宝丸组灌胃珍宝丸混悬液0.6 g/kg,模型组灌胃等体积生理盐水,1次/d.2组大鼠分别于造模后1、3、7、14 d进行行为学检测.于造模后1、3、7 d,2组分别随机抽取8只大鼠处死,分离脊髓,采用HE染色观察脊髓组织病理变化;采用硫代巴比妥酸分光光度计法测定脊髓丙二醛(MDA)水平;采用 TUNEL 染色观察细胞凋亡情况.结果 造模后1、3、7 d,与模型组比较,珍宝丸组大鼠脊髓组织MDA水平[分别为(15.12± 1.27)nmol/mg 比(19.71 ± 1.29)nmol/mg、(20.42 ± 1.33)nmol/mg 比(24.65 ± 1.36)nmol/mg、(10.46 ± 1.49)nmol/mg)比(15.46±1.44)nmol/mg)]、脊髓细胞凋亡率[(19.61±1.49)%比(26.61±1.52)%、(21.49± 1.37)%比(32.37±1.24)%、(13.04±1.30)%比(18.35±1.27)%]降低(P<0.05);造模后3、7、14 d,珍宝丸组 BBB 评分[(6.52±1.27)分比(2.63±1.27)分、(12.68±1.32)分比(6.17±1.34)分、(15.47±1.27)分比(11.57±1.29)分]高于模型组(P<0.05).结论 珍宝丸可改善大鼠ASCI后损伤段的脊髓组织病理损伤,降低MDA水平及神经细胞凋亡率,对大鼠ASCI后神经功能恢复有促进作用.
目的 探討矇藥珍寶汍對急性脊髓損傷(acute spinal cord injury, ASCI)大鼠的神經保護作用及其機製.方法 按隨機數字錶法將64隻Wistar大鼠分為模型組、珍寶汍組各32隻.採用改良Allen's重物打擊法製作胸T10水平急性脊髓不完全損傷大鼠模型.造模後30 min,珍寶汍組灌胃珍寶汍混懸液0.6 g/kg,模型組灌胃等體積生理鹽水,1次/d.2組大鼠分彆于造模後1、3、7、14 d進行行為學檢測.于造模後1、3、7 d,2組分彆隨機抽取8隻大鼠處死,分離脊髓,採用HE染色觀察脊髓組織病理變化;採用硫代巴比妥痠分光光度計法測定脊髓丙二醛(MDA)水平;採用 TUNEL 染色觀察細胞凋亡情況.結果 造模後1、3、7 d,與模型組比較,珍寶汍組大鼠脊髓組織MDA水平[分彆為(15.12± 1.27)nmol/mg 比(19.71 ± 1.29)nmol/mg、(20.42 ± 1.33)nmol/mg 比(24.65 ± 1.36)nmol/mg、(10.46 ± 1.49)nmol/mg)比(15.46±1.44)nmol/mg)]、脊髓細胞凋亡率[(19.61±1.49)%比(26.61±1.52)%、(21.49± 1.37)%比(32.37±1.24)%、(13.04±1.30)%比(18.35±1.27)%]降低(P<0.05);造模後3、7、14 d,珍寶汍組 BBB 評分[(6.52±1.27)分比(2.63±1.27)分、(12.68±1.32)分比(6.17±1.34)分、(15.47±1.27)分比(11.57±1.29)分]高于模型組(P<0.05).結論 珍寶汍可改善大鼠ASCI後損傷段的脊髓組織病理損傷,降低MDA水平及神經細胞凋亡率,對大鼠ASCI後神經功能恢複有促進作用.
목적 탐토몽약진보환대급성척수손상(acute spinal cord injury, ASCI)대서적신경보호작용급기궤제.방법 안수궤수자표법장64지Wistar대서분위모형조、진보환조각32지.채용개량Allen's중물타격법제작흉T10수평급성척수불완전손상대서모형.조모후30 min,진보환조관위진보환혼현액0.6 g/kg,모형조관위등체적생리염수,1차/d.2조대서분별우조모후1、3、7、14 d진행행위학검측.우조모후1、3、7 d,2조분별수궤추취8지대서처사,분리척수,채용HE염색관찰척수조직병리변화;채용류대파비타산분광광도계법측정척수병이철(MDA)수평;채용 TUNEL 염색관찰세포조망정황.결과 조모후1、3、7 d,여모형조비교,진보환조대서척수조직MDA수평[분별위(15.12± 1.27)nmol/mg 비(19.71 ± 1.29)nmol/mg、(20.42 ± 1.33)nmol/mg 비(24.65 ± 1.36)nmol/mg、(10.46 ± 1.49)nmol/mg)비(15.46±1.44)nmol/mg)]、척수세포조망솔[(19.61±1.49)%비(26.61±1.52)%、(21.49± 1.37)%비(32.37±1.24)%、(13.04±1.30)%비(18.35±1.27)%]강저(P<0.05);조모후3、7、14 d,진보환조 BBB 평분[(6.52±1.27)분비(2.63±1.27)분、(12.68±1.32)분비(6.17±1.34)분、(15.47±1.27)분비(11.57±1.29)분]고우모형조(P<0.05).결론 진보환가개선대서ASCI후손상단적척수조직병리손상,강저MDA수평급신경세포조망솔,대대서ASCI후신경공능회복유촉진작용.
Objective To explore the protective effect of Zhenbao pill on acute spinal cord injury of rat and its possible mechanisms.Methods 64 Wistar rats were randomly divided into a injury group and a drug group, with 32 rats in each group, A T10 acute incomplete spinal cord injury model was produced by using modified Allen technique in the two groups,Zhenbao pill mixed suspension of 0.6 g/kg was given by gavage method daily in the drug group 30 min after moding, the same amount of saline was given in the injury group. Behavior detection were conducted in the first day, third day, and the seventh day after modeling to each 8 rats of the two groups. Spinal cord specimens was got centered about the damage points after rats were executed. histopathological changes were observed under HE staining, the MDA content were determined by thiobarbituric acid spectrophotometer and the apoptotic cells were marked by TUNEL method.Results After 1, 3, 7 d, compared with the injury group, the MDA level in the rat spinal cord tissue in the drug group (15.12 ± 1.27 nmol/mgvs.19.71 ± 1.29 nmol/mg, 20.42 ± 1.33 nmol/mgvs.24.65 ±1.36 nmol/mg, 10.46±1.49 nmol/mg vs.15.46 ± 1.44 nmol/mg) decreased(P<0.05); the apoptosis rate of drug groups (19.61% ± 1.49%vs.26.61% ±1.52%, 21.49% ± 1.37% vs.32.37% ± 1.24%, 13.04% ± 1.30%vs.18.35% ± 1.27%)decreased (P<0.05); the BBB score of drug groups (6.52 ± 1.27vs. 2.63 ± 1.27), (12.68 ± 1.32 vs.6.17 ± 1.34), (15.47 ± 1.27 vs.11.57 ± 1.29) was higher than the injury group (P<0.05). Conclusion Zhenbao pill can significantly improve the histopathological examination,reduce the MDA content in the injured spinal cord specimen of the rat and the rate of neuronal apoptosis, which may promote recovery of neurologic function in the rat suffered from ASCI.
