CAJ | 학술논문

目的：研究细胞黏附分子(CAM)上皮性钙黏连蛋白(E-cad)、上皮细胞黏附分子(Ep-CAM)在头颈部腺样囊性癌(ACC)中的表达情况,探讨其在头颈部 ACC 发生、发展及侵袭转移中的作用。方法应用免疫组织化学 EnVision 法检测60例头颈部 ACC 及30例正常腺体组织中 E-cad、Ep-CAM 的表达情况,采用 Image-Pro Plus 6.0图像分析系统对免疫组织化学结果进行分析。结果E-cad 在头颈部 ACC 中的表达低于正常腺体组织(P <0.05),Ep-CAM 在头颈部 ACC 中的表达高于正常腺体组织(P <0.05)；E-cad 在头颈部 ACC 组织中的相对低表达分别与 ACC 的病例分型、临床分期、有无神经症状及有无复发转移有关(P <0.05)；Ep-CAM 在头颈部 ACC 组织中的高表达分别与 ACC 的病例分型、临床分期、有无神经症状及有无复发转移有关(P <0.05)。E-cad 与 Ep-CAM 在头颈部 ACC 中的表达呈负相关关系(r =-0.890,P =0.000)。结论E-cad 可能具有抑制头颈部 ACC 发生、发展的作用,Ep-CAM 在头颈部 ACC 中表达越高,提示头颈部 ACC 恶性程度越高。
목적：연구세포점부분자(CAM)상피성개점련단백(E-cad)、상피세포점부분자(Ep-CAM)재두경부선양낭성암(ACC)중적표체정황,탐토기재두경부 ACC 발생、발전급침습전이중적작용。방법응용면역조직화학 EnVision 법검측60례두경부 ACC 급30례정상선체조직중 E-cad、Ep-CAM 적표체정황,채용 Image-Pro Plus 6.0도상분석계통대면역조직화학결과진행분석。결과E-cad 재두경부 ACC 중적표체저우정상선체조직(P <0.05),Ep-CAM 재두경부 ACC 중적표체고우정상선체조직(P <0.05)；E-cad 재두경부 ACC 조직중적상대저표체분별여 ACC 적병례분형、림상분기、유무신경증상급유무복발전이유관(P <0.05)；Ep-CAM 재두경부 ACC 조직중적고표체분별여 ACC 적병례분형、림상분기、유무신경증상급유무복발전이유관(P <0.05)。E-cad 여 Ep-CAM 재두경부 ACC 중적표체정부상관관계(r =-0.890,P =0.000)。결론E-cad 가능구유억제두경부 ACC 발생、발전적작용,Ep-CAM 재두경부 ACC 중표체월고,제시두경부 ACC 악성정도월고。
Objective To study the expression of E-cad,Ep-CAM in head and neck ACC and clarify its role and in the malig-nant progress,invasion and metastasis of ACC.Methods The expression of E-cad and Ep-CAM in 60 cases of head and neck ACC specimens and 30 cases of normal gland tissues were detected by immunohistochemistry EnVision method.The result of immunohis-tochemistry was conducted by the Image-Pro Plus 6.0 image analysis system.Results The expression of E-cad in head and neck ACC was statistically significant lower than that in normal gland tissue(P <0.05).The expression of Ep-CAM in head and neck ACC was statistically significant higher than that in normal gland tissue(P <0.05).The decreasing expression of E-cad in head and neck ACC was closely related to the different pathological patterns,clinical stages,neurological symptom and local recurrences or distant metastases(P <0.05).The high expression of Ep-CAM in head and neck ACC was closely related to the different pathologi-cal patterns,clinical stages,neurological symptoms and local recurrences or distant metastases(P <0.05).There was a negative cor-relation about the expression between E-cad and Ep-CAM in head and neck ACC(r=-0.890,P =0.000).Conclusion E-cad may act as a inhibitor in the occurrence and development of ACC.The higher expression of Ep-CAM in head and neck ACC showed that ACC had a more poorer prognosis.