CAJ | 학술논문

目的：检测人类婆罗双树样基因-4(SALL4)在胃癌组织中的表达及其与胃癌生物学行为的关系。方法采用逆转录 PCR(RT-PCR)、Westrrn blot 和免疫组织化学方法检测91例胃癌组织和37例正常胃黏膜中 SALL4的表达,分析 SALL4与胃癌临床病理特征之间的关系。结果SALL4在胃癌组织中阳性表达率为74.7%,显著高于正常胃黏膜组织阳性表达率18.9%(P <0.05),而且随着胃癌分化程度的降低,SALL4的阳性率和表达强度逐渐增高；胃癌组织 SALL4 mRNA 和蛋白的表达水平均显著高于正常胃黏膜组织,差异有统计学意义(P <0.05)；在胃癌组织中 SALL4的阳性表达与肿瘤淋巴结转移(P =0.001)、分化程度(P =0.029)和浸润深度(P =0.050)密切相关。结论SALL4基因在胃癌组织中高表达,与淋巴结转移、分化程度和浸润深度密切相关,可能在胃癌发生、发展中起重要的作用。
목적：검측인류파라쌍수양기인-4(SALL4)재위암조직중적표체급기여위암생물학행위적관계。방법채용역전록 PCR(RT-PCR)、Westrrn blot 화면역조직화학방법검측91례위암조직화37례정상위점막중 SALL4적표체,분석 SALL4여위암림상병리특정지간적관계。결과SALL4재위암조직중양성표체솔위74.7%,현저고우정상위점막조직양성표체솔18.9%(P <0.05),이차수착위암분화정도적강저,SALL4적양성솔화표체강도축점증고；위암조직 SALL4 mRNA 화단백적표체수평균현저고우정상위점막조직,차이유통계학의의(P <0.05)；재위암조직중 SALL4적양성표체여종류림파결전이(P =0.001)、분화정도(P =0.029)화침윤심도(P =0.050)밀절상관。결론SALL4기인재위암조직중고표체,여림파결전이、분화정도화침윤심도밀절상관,가능재위암발생、발전중기중요적작용。
Objective To study the expression and clinical significance of the SALL4 in human gastric carcinoma tissues. Methods The expression of SALL4 in 91 samples of gastric carcinoma and 37 samples of normal gastric tissues was detected by RT-PCR,Western blot and immunohistochemistry,and its relationship with the clinical data were analyzed statistically.Results The positive expression rate of SALL4 in gastric carcinoma(74.7%)was significantly higher than that(18.9%)in normal gastric mucosa tissues(P <0.05).Moreover,with the decreased with the differentiation of gastric carcinoma,the positive expression rate of SALL4 was increased.The expression of SALL4 mRNA and protein in gastric carcinoma tissues were significantly higher than that in normal gastric tissues(P <0.050).The expression levels of SALL4 were relevant to lymph node metastasis(P =0.001),infiltra-tion depth(P =0.029)and the differentiation degree of gastric carcinoma(P =0.050).Conclusion SALL4 was highly expressed in gastric cancer tissues and relevant to lymph node metastasis,infiltration depth and the differentiation degree,which may have play an important role in the development of gastric cancer.