CAJ | 학술논문

目的研究北五味子木脂素( Schisandra Chinensis Baill Lignans,SCL)抗焦虑的作用及机制.方法采用明暗箱试验和高架十字迷宫试验,观察北五味子木脂素低、中、高剂量(35,70,140 mg/kg)灌胃给药7 d后对小鼠行为的影响;另设空白对照组(给予生理盐水)和地西泮(2 mg/kg)阳性对照组.用酶联免疫吸附法( ELISA)测定小鼠血清γ-氨基丁酸( GABA)的含量.结果明暗箱试验和高架十字迷宫试验结果显示:北五味子木脂素中、高剂量组具有显著的抗焦虑作用(P<0. 05),并呈剂量依赖关系,这种抗焦虑作用可被氟马西尼所拮抗.酶联免疫吸附试验显示:中、高剂量组小鼠血清中γ-氨基丁酸( GABA)的含量显著增高.结论北五味子木脂素有显著的抗焦虑作用,其机制可能是通过γ-氨基丁酸受体(γ-aminobutyric acid,GABA-R)而发挥作用.
목적 연구북오미자목지소( Schisandra Chinensis Baill Lignans,SCL)항초필적작용급궤제.방법 채용명암상시험화고가십자미궁시험,관찰북오미자목지소저、중、고제량(35,70,140 mg/kg)관위급약7 d후대소서행위적영향;령설공백대조조(급여생리염수)화지서반(2 mg/kg)양성대조조.용매련면역흡부법( ELISA)측정소서혈청γ-안기정산( GABA)적함량.결과 명암상시험화고가십자미궁시험결과현시:북오미자목지소중、고제량조구유현저적항초필작용(P<0. 05),병정제량의뢰관계,저충항초필작용가피불마서니소길항.매련면역흡부시험현시:중、고제량조소서혈청중γ-안기정산( GABA)적함량현저증고.결론 북오미자목지소유현저적항초필작용,기궤제가능시통과γ-안기정산수체(γ-aminobutyric acid,GABA-R)이발휘작용.
Objective To study the anti-anxiety function and mechanism of Schisandra chinensis Baill Lignans ( SCL) . Method Light-dark box test and elevated plus maze test were used to observe the influence of Schisandra chinensis Baill Lignans on mice behaviors administrated intragastrically at low,medium and high dose (35,70 and 140 mg/kg) after 7 days. The blank control group and the positive control group were given normal saline and diazepam(2 mg/kg),respectively. Enzyme-linked immunoabsorbent assay(ELISA) was used for the detection of gamma aminobutyric acid(GABA) in mice serum. Results Light-dark box test and elevated plus maze test results showed that SCL had significant anti-anxiety function at medium and high dose(P<0. 05) with a dose-response relationship. And this kind of anti-anxiety effect could be antagonized by flumazenil. ELISA experiments showed that serum gamma aminobutyric acid ( GABA ) levels of the mice in the medium and high dose group increased significantly. Conclusion Schisandra chinensis Baill Lignans showed significantly anti-anxiety effect,its mechanism may be related with gamma-aminobutyric acid receptor( gamma aminobutyric acid, GABA-R) .