北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
Journal of Beihua University(Natural Science)
2015年
5期
605-608
,共4页
盛瑜%孙景昱%李明成%张丽华%李洪宇%李梓僮%苑广信
盛瑜%孫景昱%李明成%張麗華%李洪宇%李梓僮%苑廣信
성유%손경욱%리명성%장려화%리홍우%리재동%원엄신
梅花鹿鞭%马鹿鞭%牛鞭%RAPD%指纹图谱
梅花鹿鞭%馬鹿鞭%牛鞭%RAPD%指紋圖譜
매화록편%마록편%우편%RAPD%지문도보
Gervus nippon Temminck whip%cervus elaphus Linnaeus whip%bull whip%RAPD%fingerprint
目的 建立鹿鞭及其伪品随机扩增DNA多态性( RAPD)鉴定方法,为鹿鞭的鉴别提供可靠依据.方法 采用柱层析法从鹿鞭及其伪品中提取线粒体DNA并进行随机DNA多态性扩增.结果 正品鹿鞭(梅花鹿鞭和马鹿鞭)与其常见伪品(牛鞭)线粒体DNA随机扩增产物的电泳图谱有明显区别,条带数量和位置均不同,说明本方法可以有效区分正品鹿鞭与其常见伪品.结论 RAPD技术可为鹿鞭及其伪品的鉴别提供一种简单、有效、可信度高的方法.
目的 建立鹿鞭及其偽品隨機擴增DNA多態性( RAPD)鑒定方法,為鹿鞭的鑒彆提供可靠依據.方法 採用柱層析法從鹿鞭及其偽品中提取線粒體DNA併進行隨機DNA多態性擴增.結果 正品鹿鞭(梅花鹿鞭和馬鹿鞭)與其常見偽品(牛鞭)線粒體DNA隨機擴增產物的電泳圖譜有明顯區彆,條帶數量和位置均不同,說明本方法可以有效區分正品鹿鞭與其常見偽品.結論 RAPD技術可為鹿鞭及其偽品的鑒彆提供一種簡單、有效、可信度高的方法.
목적 건립록편급기위품수궤확증DNA다태성( RAPD)감정방법,위록편적감별제공가고의거.방법 채용주층석법종록편급기위품중제취선립체DNA병진행수궤DNA다태성확증.결과 정품록편(매화록편화마록편)여기상견위품(우편)선립체DNA수궤확증산물적전영도보유명현구별,조대수량화위치균불동,설명본방법가이유효구분정품록편여기상견위품.결론 RAPD기술가위록편급기위품적감별제공일충간단、유효、가신도고적방법.
Objective To establish the identification method of Penis Cervi from its adulterants by RAPD technology,and to provide a reliable evidence for Penis Cervi identification. Method Column chromatography method was used to extract and purify mt DNAs from Penis Cervi and its adulterants,and the DNA fragments were amplified by RAPD technology. Results The results showed that there was a great difference in the number and brightness of the stripes of RAPD products between Penis Cervi ( Gervus nippon Temminck whip and Cervus elaphus Linnaeus whip) and its adulterants(Bull whip). Conclusion The technology of RAPD could be a simple,effective and reliable method for the identification of Penis Cervi from its adulterants.
