CAJ | 학술논문

目的探讨大鼠在亚高原环境及高原环境下力竭运动后不同时间肾损伤程度的变化,以及对急性肾损伤( acute kidney injury, AKI)各指标进行比较,初步评价不同指标检测AKI的灵敏度与可靠性. 方法 72只大鼠随机分为亚高原组和高原组,每组36只,再将两组大鼠分别随机分为6个亚组,每组6只,各组取样时间不同. 通过对大鼠进行负重游泳至力竭,记录游泳时间,检测力竭后不同时间血清中肌酐、尿素氮以及中性粒细胞明胶酶相关纸质运载蛋白( neutrophil gelatnase associated lipocalin, NGAL)的含量. 结果高原组力竭游泳时间明显比亚高原组短( P<0. 05);亚高原组血清肌酐及尿素氮在力竭后12 h时升高(P<0. 01),血清肌酐48 h时依然维持高浓度(P<0. 01),而尿素氮在24 h时恢复到对照组水平,高原组血清肌酐及尿素氮在力竭运动后即刻升高( P<0. 01 ) ,6 h后恢复到对照组水平,血清肌酐48 h后又显著升高(P<0. 05),血清尿素氮12 h时又显著升高(P<0. 05),48 h时恢复到对照组水平. 亚高原及高原组血清NGAL在力竭后12 h时升高(P<0. 05,P<0. 01),在24 h、48 h持续升高. 结论高原环境可使大鼠运动能力降低,血清中肌酐、尿素氮以及NGAL都可表征AKI,但NGAL灵敏度高、可靠性强,具有极高的稳定性.
목적 탐토대서재아고원배경급고원배경하력갈운동후불동시간신손상정도적변화,이급대급성신손상( acute kidney injury, AKI)각지표진행비교,초보평개불동지표검측AKI적령민도여가고성. 방법 72지대서수궤분위아고원조화고원조,매조36지,재장량조대서분별수궤분위6개아조,매조6지,각조취양시간불동. 통과대대서진행부중유영지력갈,기록유영시간,검측력갈후불동시간혈청중기항、뇨소담이급중성립세포명효매상관지질운재단백( neutrophil gelatnase associated lipocalin, NGAL)적함량. 결과 고원조력갈유영시간명현비아고원조단( P<0. 05);아고원조혈청기항급뇨소담재력갈후12 h시승고(P<0. 01),혈청기항48 h시의연유지고농도(P<0. 01),이뇨소담재24 h시회복도대조조수평,고원조혈청기항급뇨소담재력갈운동후즉각승고( P<0. 01 ) ,6 h후회복도대조조수평,혈청기항48 h후우현저승고(P<0. 05),혈청뇨소담12 h시우현저승고(P<0. 05),48 h시회복도대조조수평. 아고원급고원조혈청NGAL재력갈후12 h시승고(P<0. 05,P<0. 01),재24 h、48 h지속승고. 결론 고원배경가사대서운동능력강저,혈청중기항、뇨소담이급NGAL도가표정AKI,단NGAL령민도고、가고성강,구유겁고적은정성.
Objective To investigate changes of acute kidney injury ( AKI) after exhausting excise under high altitude and sub-high altitude environments for different times in rats, and to evaluate the sensitivity and reliability by dif-ferent indexes detection for AKI after comparing AKI indexes. Methods A total of 72 rats were randomly divided into high altitude group (group A, n=36) and sub-high altitude group (group B, n=36), and then each group was random-ly divided into six subgroups ( n =6 for each subgroup ) by taking samples with different times. The rats underwent weight-bearing swimming test till exhaustion, and then the swimming time was recorded, and the values of serum creati-nine, urea nitrogen and neutrophil gelatinase-associated lipocalin ( NGAL) content in different times after exhaustion were detected. Results The swimming time of group A was significantly shorter than that of group B (P<0. 05). In group B, the values of serum creatinine and urea were significantly increased at 12 h after exhausting exercise (P<0. 01);the concentration of serum creatinine kept high at 48 h after exhausting exercise (P<0. 01), while serum urea value restored to the level of control group at 24 h after exhausting exercise. In group A, the values of serum creatinine and urea were increased immediately after exhausting exercise (P<0. 01), and then the values returned to the level of control group at 6 h after exhausting exercise, while serum creatinine value was significantly increased at 48 h after exhausting exercise (P<0. 05), and serum urea value was increased at 24 h after exhausting exercise (P<0. 05), and then the value re-stored to the level of control group at 48 h after exhausting exercise. Values of serum NGAL were increased at 12 h after exhausting exercise in the two groups (P<0. 05, P<0. 01), and the values were still increasing at 24 h and 48 h after exhausting exercise. Conclusion The motor abilities of rats can be decreased in high altitude environment. Serum creat-inine, urea and NGAL can be markers of acute kidney injury, but NGAL shows better sensitivity, reliability and stability.