北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
Journal of Beihua University(Natural Science)
2015年
5期
601-604
,共4页
肝损伤%前列腺素2%肿瘤坏死因子%D-半乳糖%白介素6
肝損傷%前列腺素2%腫瘤壞死因子%D-半乳糖%白介素6
간손상%전렬선소2%종류배사인자%D-반유당%백개소6
liver injury%prostaglandin 2%tumor necrois factor%D-galactose%Interleukin 6
目的 探讨应用D-半乳糖( D-gal)建立小鼠肝脏损伤模型的方法.方法 将20只小鼠随机分为两组,正常对照组应用生理盐水20 mL/( kg·d-1 )皮下注射,模型组应用D-半乳糖按500 mg/( kg·d-1 ) 皮下注射.造模完成后采用比色法测定血清AST,ALT及GST;放免法测定血浆PGE2,TNF及IL-6;Western blot方法检测肝组织COX-2表达.通过光镜观察肝组织病理变化.结果 血浆ALT,AST及GST模型组与正常对照组比较有明显升高(P<0. 01);模型组与正常对照组比较血浆PGE2,TNF及IL-6升高明显,差异具有统计学意义(P<0. 01);病理切片显示肝脏结构呈现病理改变.结论 应用D-半乳糖可以成功制作小鼠肝脏损伤模型.
目的 探討應用D-半乳糖( D-gal)建立小鼠肝髒損傷模型的方法.方法 將20隻小鼠隨機分為兩組,正常對照組應用生理鹽水20 mL/( kg·d-1 )皮下註射,模型組應用D-半乳糖按500 mg/( kg·d-1 ) 皮下註射.造模完成後採用比色法測定血清AST,ALT及GST;放免法測定血漿PGE2,TNF及IL-6;Western blot方法檢測肝組織COX-2錶達.通過光鏡觀察肝組織病理變化.結果 血漿ALT,AST及GST模型組與正常對照組比較有明顯升高(P<0. 01);模型組與正常對照組比較血漿PGE2,TNF及IL-6升高明顯,差異具有統計學意義(P<0. 01);病理切片顯示肝髒結構呈現病理改變.結論 應用D-半乳糖可以成功製作小鼠肝髒損傷模型.
목적 탐토응용D-반유당( D-gal)건립소서간장손상모형적방법.방법 장20지소서수궤분위량조,정상대조조응용생리염수20 mL/( kg·d-1 )피하주사,모형조응용D-반유당안500 mg/( kg·d-1 ) 피하주사.조모완성후채용비색법측정혈청AST,ALT급GST;방면법측정혈장PGE2,TNF급IL-6;Western blot방법검측간조직COX-2표체.통과광경관찰간조직병리변화.결과 혈장ALT,AST급GST모형조여정상대조조비교유명현승고(P<0. 01);모형조여정상대조조비교혈장PGE2,TNF급IL-6승고명현,차이구유통계학의의(P<0. 01);병리절편현시간장결구정현병리개변.결론 응용D-반유당가이성공제작소서간장손상모형.
Objective To explore the application of D-galactose in producing liver injury model in mice. Method 20 mouse were randomly divided into 2 groups. The normal control group was injected saline 20 mL/( kg · d-1 ) subcutaneously, and the model group was administrated D-galactose 500 mg/( kg · d-1 ) subcutaneously. After the model was established,colorimetric method was used to determine serum AST,ALT and GST,plasma concentrations of PGE2 TNF and IL-6 were measured by radio immunity, and the expression of COX-2 was detected by Western blot. Meanwhile, pathological change of the liver tissue was observed through light microscope. Results Compared with the normal control group, serum ALT, AST and GST of the model group increased significantly(P<0. 01),plasma PGE2,TNF and IL-6 also increased obviously with statistical significance(P<0. 01). Pathological section indicated clear changes in liver tissue of the mice in model group. Conclusion D-galactose can successfully establish liver injury model in mice.
