北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
Journal of Beihua University(Natural Science)
2015年
5期
597-600
,共4页
李春颖%李菁%李东盈%曲娴
李春穎%李菁%李東盈%麯嫻
리춘영%리정%리동영%곡한
动脉粥样硬化%茶多酚%血管内皮依赖性舒张%大鼠
動脈粥樣硬化%茶多酚%血管內皮依賴性舒張%大鼠
동맥죽양경화%다다분%혈관내피의뢰성서장%대서
arteriosclerosis%tea polyphenol%endothelium-dependent vasodilatation%rats
目的 通过复制大鼠动脉粥样硬化( Arteriosclerosis,AS)模型,观察茶多酚对AS大鼠血管内皮功能的影响.方法 将40只Wistar大鼠随机分为正常组(NC组)、模型组(AS组)、茶多酚低剂量组(TPL组,0. 2 g/kg)、茶多酚高剂量组(TPH组,0. 4 g/kg),9周后测定血管内皮依赖性舒张功能.结果 1)血管内皮依赖性舒张功能:AS组与NC相比,大鼠胸主动脉血管环在10-9 ~10-4 mol/L浓度的Ach溶液中血管内皮舒张率明显降低( P<0. 01);TPL,TPH与AS组相比,大鼠胸主动脉血管环在10-9 ~10-4 mol/L浓度的Ach溶液中内皮舒张率明显升高(P<0. 05);2)血管内膜/中膜厚度比:AS组与NC相比,内膜/中膜厚度比明显增大(P<0. 01),具有统计学意义;TPL,TPH与AS组比较,内膜/中膜厚度比明显降低(P<0. 01);3)主动脉形态学观察:AS组大鼠动脉壁可见多处隆起于内膜表面的典型粥样斑块;动脉内皮细胞脱落,内膜增厚、断裂;中膜平滑肌明显萎缩,排列不规整. NC组无形态学改变,其他各组改变较轻,未见粥样斑块形成.结论 茶多酚具有保护血管内皮细胞的功能.
目的 通過複製大鼠動脈粥樣硬化( Arteriosclerosis,AS)模型,觀察茶多酚對AS大鼠血管內皮功能的影響.方法 將40隻Wistar大鼠隨機分為正常組(NC組)、模型組(AS組)、茶多酚低劑量組(TPL組,0. 2 g/kg)、茶多酚高劑量組(TPH組,0. 4 g/kg),9週後測定血管內皮依賴性舒張功能.結果 1)血管內皮依賴性舒張功能:AS組與NC相比,大鼠胸主動脈血管環在10-9 ~10-4 mol/L濃度的Ach溶液中血管內皮舒張率明顯降低( P<0. 01);TPL,TPH與AS組相比,大鼠胸主動脈血管環在10-9 ~10-4 mol/L濃度的Ach溶液中內皮舒張率明顯升高(P<0. 05);2)血管內膜/中膜厚度比:AS組與NC相比,內膜/中膜厚度比明顯增大(P<0. 01),具有統計學意義;TPL,TPH與AS組比較,內膜/中膜厚度比明顯降低(P<0. 01);3)主動脈形態學觀察:AS組大鼠動脈壁可見多處隆起于內膜錶麵的典型粥樣斑塊;動脈內皮細胞脫落,內膜增厚、斷裂;中膜平滑肌明顯萎縮,排列不規整. NC組無形態學改變,其他各組改變較輕,未見粥樣斑塊形成.結論 茶多酚具有保護血管內皮細胞的功能.
목적 통과복제대서동맥죽양경화( Arteriosclerosis,AS)모형,관찰다다분대AS대서혈관내피공능적영향.방법 장40지Wistar대서수궤분위정상조(NC조)、모형조(AS조)、다다분저제량조(TPL조,0. 2 g/kg)、다다분고제량조(TPH조,0. 4 g/kg),9주후측정혈관내피의뢰성서장공능.결과 1)혈관내피의뢰성서장공능:AS조여NC상비,대서흉주동맥혈관배재10-9 ~10-4 mol/L농도적Ach용액중혈관내피서장솔명현강저( P<0. 01);TPL,TPH여AS조상비,대서흉주동맥혈관배재10-9 ~10-4 mol/L농도적Ach용액중내피서장솔명현승고(P<0. 05);2)혈관내막/중막후도비:AS조여NC상비,내막/중막후도비명현증대(P<0. 01),구유통계학의의;TPL,TPH여AS조비교,내막/중막후도비명현강저(P<0. 01);3)주동맥형태학관찰:AS조대서동맥벽가견다처륭기우내막표면적전형죽양반괴;동맥내피세포탈락,내막증후、단렬;중막평활기명현위축,배렬불규정. NC조무형태학개변,기타각조개변교경,미견죽양반괴형성.결론 다다분구유보호혈관내피세포적공능.
Objective To observe the effect of tea polyphenols on endothelial function in arteriosclerosis rats (AS) model. Method A total of 40 Wistar rats were randomly divided into normal group(NC group),model group(AS group),tea polyphenols low dose group(TPL group,0. 2 g/kg),tea polyphenols high dose group(TPH group,0. 4 g/kg),and determination after 9 weeks,the vascular endothelial dependent diastolic function was determined. Results (1) Endothelium dependent diastolic function:In 10-9 ~ 10-4 mol / L Ach solution,the vascular endothelial relaxation rate of thoracic aorta ring in AS group significantly decreased compared with NC group,(P<0. 01),and the relaxation rate of TPL and TPH group showed obvious increases compared with AS group(P<0. 05). (2) Vascular intima/media thickness ratio: The intima/media thickness ratio of AS group significantly increased compared with NC group ( P<0. 01 ), and the ration of TPL and TPH group were significantly lower than AS group(P<0. 01). (3) Observations on aortic morphology: In AS group,the rat's arterial wall showed many typical arteriosclerosis plaques bulging from the surface of intima with evfoliation of arterial endothelial cells, thickening and breaking of intima. Smooth muscle of tunica media showed marked atrophy with irregular arrangement. There was no morphological change in NC group, and were milder changes with no arteriosclerosis plagues formation in other groups. Conclusion Tea polyphenols could protect vascular endothelial cells.
