北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
Journal of Beihua University(Natural Science)
2015年
5期
593-596
,共4页
张吉林%常哲兴%宋宇%熊英%宋玉国
張吉林%常哲興%宋宇%熊英%宋玉國
장길림%상철흥%송우%웅영%송옥국
肿瘤%T细胞抗原受体%克隆化改变
腫瘤%T細胞抗原受體%剋隆化改變
종류%T세포항원수체%극륭화개변
tumor%T cell antigen receptor%cloning change
目的 探讨肿瘤患者T细胞抗原受体( TCR) Vβ基因克隆化改变特征.方法 应用多引物巢式PCR技术检测肿瘤患者外周血CD4+T细胞和CD8+T细胞TCR Vβ基因22个亚家族的克隆化改变,与正常对照组比较,分析肿瘤患者TCR单克隆改变的特点.结果 CD8+T细胞TCRVβ基因亚家族单克隆改变的数量多于CD4+T细胞;肿瘤患者的CD4+T细胞中,只有Vβ2,Vβ7和Vβ8三个亚家族单克隆改变高于正常对照组( P<0. 01或P<0. 05),其他Vβ亚家族单克隆改变与正常对照组无明显差异.肿瘤患者的CD8+T细胞中,有14个Vβ亚家族单克隆改变均高于正常对照组(P<0. 01或P<0. 05);4组肿瘤患者中,CD4+T细胞和CD8+T细胞的TCR Vβ7亚家族的单克隆改变均明显高于正常对照组(P<0. 01或P<0. 05).结论 通过检测TCR Vβ基因克隆化改变,可以初步了解T细胞对肿瘤的免疫应答状况.
目的 探討腫瘤患者T細胞抗原受體( TCR) Vβ基因剋隆化改變特徵.方法 應用多引物巢式PCR技術檢測腫瘤患者外週血CD4+T細胞和CD8+T細胞TCR Vβ基因22箇亞傢族的剋隆化改變,與正常對照組比較,分析腫瘤患者TCR單剋隆改變的特點.結果 CD8+T細胞TCRVβ基因亞傢族單剋隆改變的數量多于CD4+T細胞;腫瘤患者的CD4+T細胞中,隻有Vβ2,Vβ7和Vβ8三箇亞傢族單剋隆改變高于正常對照組( P<0. 01或P<0. 05),其他Vβ亞傢族單剋隆改變與正常對照組無明顯差異.腫瘤患者的CD8+T細胞中,有14箇Vβ亞傢族單剋隆改變均高于正常對照組(P<0. 01或P<0. 05);4組腫瘤患者中,CD4+T細胞和CD8+T細胞的TCR Vβ7亞傢族的單剋隆改變均明顯高于正常對照組(P<0. 01或P<0. 05).結論 通過檢測TCR Vβ基因剋隆化改變,可以初步瞭解T細胞對腫瘤的免疫應答狀況.
목적 탐토종류환자T세포항원수체( TCR) Vβ기인극륭화개변특정.방법 응용다인물소식PCR기술검측종류환자외주혈CD4+T세포화CD8+T세포TCR Vβ기인22개아가족적극륭화개변,여정상대조조비교,분석종류환자TCR단극륭개변적특점.결과 CD8+T세포TCRVβ기인아가족단극륭개변적수량다우CD4+T세포;종류환자적CD4+T세포중,지유Vβ2,Vβ7화Vβ8삼개아가족단극륭개변고우정상대조조( P<0. 01혹P<0. 05),기타Vβ아가족단극륭개변여정상대조조무명현차이.종류환자적CD8+T세포중,유14개Vβ아가족단극륭개변균고우정상대조조(P<0. 01혹P<0. 05);4조종류환자중,CD4+T세포화CD8+T세포적TCR Vβ7아가족적단극륭개변균명현고우정상대조조(P<0. 01혹P<0. 05).결론 통과검측TCR Vβ기인극륭화개변,가이초보료해T세포대종류적면역응답상황.
Objective To investigate the character of T cell antigen receptor( TCR) Vβgene cloning changes in the patients. Method Multi-primers nested PCR technique was applied to detect the cloning changes of 22 subfamily of CD4+ T cells and CD8+ T cell TCR Vβ gene in tumor patients. The results were compared with the normal control group,and the TCR monoclonal change features in cancer patients were analyzed. Results The number of CD8+ T cells TCR Vβ gene subfamily monoclonal change was more than that of CD4+ T cells. In the tumor patients CD4+ T cells,only three subfamilies(Vβ2,Vβ7 and Vβ8) monoclonal changes were higher than that in the control group(P<0. 01 or P<0. 05). As for other Vβ subfamily monoclonal changes,no significant difference was observed compared with the control group. For CD8+ T cells of the tumor patients,there were 14 Vβ subfamilies monoclonal change were higher than that of the normal control group ( P<0 . 01 or P<0 . 05 ) . Among of four groups of tumor patients,Vβ7 subfamilies monoclonal changes in both CD4+ T cells and CD8+ T cell TCR were significantly higher than that of the control group ( P<0 . 01 or P<0 . 05 ) . Conclusion By detecting TCR Vβ gene monoclonal change, the situation of T cell immune response to the tumor could be preliminary understood.
