解放军医药杂志
解放軍醫藥雜誌
해방군의약잡지
Medical & Pharmaceutical Journal of Chinese People's Liberation Army
2015年
9期
1-6
,共6页
惠玲%王晓辉%王美亮%杨霄鹏%马明仁%桑春艳%李君红%张富婷
惠玲%王曉輝%王美亮%楊霄鵬%馬明仁%桑春豔%李君紅%張富婷
혜령%왕효휘%왕미량%양소붕%마명인%상춘염%리군홍%장부정
多巴胺%多巴胺激动剂%多巴胺拮抗剂%LMO3%CIB1
多巴胺%多巴胺激動劑%多巴胺拮抗劑%LMO3%CIB1
다파알%다파알격동제%다파알길항제%LMO3%CIB1
Dopamine%Dopamine agonists%Dopamine antagonists%LMO3%CIB1
目的 了解多巴胺对C8胶质细胞生长的影响、可能受体途径以及对CIB1、LMO3表达的影响. 方法 采用MTT方法检测多巴胺、多巴胺受体激动剂、多巴胺受体拮抗剂对C8胶质细胞生长增殖的作用,同时采用免疫荧光组化法检测不同浓度多巴胺对C8胶质细胞中LMO3、CIB1表达及定位的影响. 结果 多巴胺对C8胶质细胞的作用与浓度相关,低浓度(10 μmol/L)对C8胶质细胞生长无明显影响,中浓度(100 μmol/L)可明显促进C8胶质细胞的增殖(P<0. 05,P<0. 01),而高浓度(500 μmol/L)则抑制C8胶质细胞生长(P<0. 05);多巴胺处理组比多巴胺D1激动剂组促C8胶质细胞生长作用明显,同时加入多巴胺及D2受体拮抗剂组比多巴胺处理组C8胶质细胞显著增加( P<0. 01). 低浓度(10 μmol/L)多巴胺显著增加C8胶质细胞LMO3的表达水平(P<0. 05);高浓度(500 μmol/L)多巴胺则降低CIB1蛋白的表达(P<0. 05). 结论 多巴胺主要通过多巴胺D2受体对C8胶质细胞生长增殖起作用,并呈浓度相关性,多巴胺D2受体拮抗剂对C8胶质细胞促增殖作用有协同性,不同浓度多巴胺对C8胶质细胞中CIB1及LMO3的表达影响不同,具体作用机制还有待进一步研究.
目的 瞭解多巴胺對C8膠質細胞生長的影響、可能受體途徑以及對CIB1、LMO3錶達的影響. 方法 採用MTT方法檢測多巴胺、多巴胺受體激動劑、多巴胺受體拮抗劑對C8膠質細胞生長增殖的作用,同時採用免疫熒光組化法檢測不同濃度多巴胺對C8膠質細胞中LMO3、CIB1錶達及定位的影響. 結果 多巴胺對C8膠質細胞的作用與濃度相關,低濃度(10 μmol/L)對C8膠質細胞生長無明顯影響,中濃度(100 μmol/L)可明顯促進C8膠質細胞的增殖(P<0. 05,P<0. 01),而高濃度(500 μmol/L)則抑製C8膠質細胞生長(P<0. 05);多巴胺處理組比多巴胺D1激動劑組促C8膠質細胞生長作用明顯,同時加入多巴胺及D2受體拮抗劑組比多巴胺處理組C8膠質細胞顯著增加( P<0. 01). 低濃度(10 μmol/L)多巴胺顯著增加C8膠質細胞LMO3的錶達水平(P<0. 05);高濃度(500 μmol/L)多巴胺則降低CIB1蛋白的錶達(P<0. 05). 結論 多巴胺主要通過多巴胺D2受體對C8膠質細胞生長增殖起作用,併呈濃度相關性,多巴胺D2受體拮抗劑對C8膠質細胞促增殖作用有協同性,不同濃度多巴胺對C8膠質細胞中CIB1及LMO3的錶達影響不同,具體作用機製還有待進一步研究.
목적 료해다파알대C8효질세포생장적영향、가능수체도경이급대CIB1、LMO3표체적영향. 방법 채용MTT방법검측다파알、다파알수체격동제、다파알수체길항제대C8효질세포생장증식적작용,동시채용면역형광조화법검측불동농도다파알대C8효질세포중LMO3、CIB1표체급정위적영향. 결과 다파알대C8효질세포적작용여농도상관,저농도(10 μmol/L)대C8효질세포생장무명현영향,중농도(100 μmol/L)가명현촉진C8효질세포적증식(P<0. 05,P<0. 01),이고농도(500 μmol/L)칙억제C8효질세포생장(P<0. 05);다파알처리조비다파알D1격동제조촉C8효질세포생장작용명현,동시가입다파알급D2수체길항제조비다파알처리조C8효질세포현저증가( P<0. 01). 저농도(10 μmol/L)다파알현저증가C8효질세포LMO3적표체수평(P<0. 05);고농도(500 μmol/L)다파알칙강저CIB1단백적표체(P<0. 05). 결론 다파알주요통과다파알D2수체대C8효질세포생장증식기작용,병정농도상관성,다파알D2수체길항제대C8효질세포촉증식작용유협동성,불동농도다파알대C8효질세포중CIB1급LMO3적표체영향불동,구체작용궤제환유대진일보연구.
Objective To investigate the effects of Dopamine on the proliferation of C8 glial cells, the possible acceptor pathway and the expressions of CIB1 and LMO3. Methods The effects of Dopamine, Dopamine Agonists and Dopamine Receptor Antagonists on proliferation of C8 glial cells was detected using MTT ( thiazolyl blue) assay, and the effects of different concentrations of Dopamine on expressions and locations of LMO3 and CIB1 in C8 glial cells were de-tected using immunofluorescence method. Results The effects of Dopamine on C8 glial cells obviously related with the its concentration. Low-concentration (10μmol/L) group had no significant effect on cell growth, middle-concentration (100 μmol/L) group significantly enhanced cell proliferation(P < 0.05, P < 0.01), while high-concentration (500 μmol/L) group inhibited the cell growth (P<0. 05). Dopamine-treated group was found more significantly effect on the cell growth than that in D1 agonist group, while the number of C8 glial cells were significant increased by treated with Dopamine and D2 receptor antagonist than by Dopamine ( P<0. 01 ) . The LMO3 expression was significantly in-creased in low-concentration group (P<0. 05), while the expression of CIB1 protein was decreased in high-concentration group (P<0. 05). Conclusion The Dopamine may affect the proliferation of C8 glial cells by Dopamine D2 receptors, and it closely relates to Dopamine concentrations. D2 Dopamine receptor antagonist has the cooperativity with the prolifer-ation of C8 glial cells, and different concentrations of Dopamine can affect expressions of CIB1 and LMO3 in C8 glial cells in different degree.
