CAJ | 학술논문

目的：利用人脐带间充质干细胞造血支持的特性，优化人胚胎干细胞造血分化方案。方法利用组织块培养法分离扩增人脐带间充质干细胞，收集条件培养液。以小鼠胚胎成纤维细胞为饲养层培养人胚胎干细胞，再用拟胚体培养液诱导拟胚体形成。分别用条件培养液和拟胚体培养液培养拟胚体，再用流式细胞术检测EB中造血标志CD45和CD34的表达。结果利用组织块培养法获得大量形态均一、增殖迅速的脐带间充质干细胞；间充质干细胞条件培养液诱导8 d后，拟胚体中的CD45表达60％以上，CD34表达30％以上，远高于含有BM P4的拟胚体培养液的诱导率。结论人脐带间充质干细胞条件培养液可促进人胚胎干细胞的造血分化，并避免动物源污染，节约成本。
목적：이용인제대간충질간세포조혈지지적특성，우화인배태간세포조혈분화방안。방법이용조직괴배양법분리확증인제대간충질간세포，수집조건배양액。이소서배태성섬유세포위사양층배양인배태간세포，재용의배체배양액유도의배체형성。분별용조건배양액화의배체배양액배양의배체，재용류식세포술검측EB중조혈표지CD45화CD34적표체。결과이용조직괴배양법획득대량형태균일、증식신속적제대간충질간세포；간충질간세포조건배양액유도8 d후，의배체중적CD45표체60％이상，CD34표체30％이상，원고우함유BM P4적의배체배양액적유도솔。결론인제대간충질간세포조건배양액가촉진인배태간세포적조혈분화，병피면동물원오염，절약성본。
Objective To optimize the scheme of human embryonic stem cell differentiation to hematopoietic cell by taking advantage of the characteristics of umbilical cord mesenchymal stem cells in supporting hematopoiesis . Method The umbilical cord mesenchymal stem cells were isolated and expanded with tissue block culture method and then the condition medium were collected routinely .Human embryonic stem cell were cultured on mouse embryonic fibroblasts ,and were induced to be embryoid bodies with embryoid bodies culture medium .The embryoid bodies were cultured with condition medium and embryoid bodies culture medium respectively .Finally ,the percentage of hematopoietic cell maker CD45 and CD34 were detected with flow cytometry .Result It is obtained a large number of homogeneous fibroblasts‐like umbilical cord mesenchymal stem cells which proliferated rapidly .After the embryoid bodies were cultured with condition medium 8 days ,the result shown that the percentage of CD45 were more than 60% and CD34 were more than 30% ,far higher than that of embryoidbodies cultured with embryoid bodies culture medium .Conclusion Human umbilical cord mesenchymal stem cells conditioned medium can promote thehematopoiet‐ic differentiation of human embryonic stem cells .And his method will avoid the mouse‐related disease and cut down the cost of experiment .