CAJ | 학술논문

目的探讨高压氧预处理对皮瓣移植术后大鼠缺血再灌注损伤炎症反应的影响.方法将SD大鼠56只按随机数字表法分为假手术组(SH,n=8),缺血再灌注组(IR,n=24)和高压氧预处理后缺血再灌注组(HBO,n=24),IR组和HBO组再根据取血的时间各分3个亚组(IRld、3d、5d组和HBO 1 d、3d、5d组),每个亚组8只大鼠.HBO 1 d、3d、5d组于造模前3d进行高压氧预处理,每天2次(间隔12 h),连续干预3d.7组均建立腹部带蒂移植皮瓣动物模型,SH组不夹闭或切断动静脉.造模成功后,SH组于术后第3天,其余6组则于对应的时间点应用酶联接免疫吸附剂测定(ELISA)法检测各组大鼠血清IL-23水平;同时采用组织损伤评分对SH组及IR3d、5d组和HBO 3 d、5d组的成活皮瓣进行组织学评分.结果经高压氧预处理后,HBO 3 d组IL-23水平为(17.80±14.78) ng/ml,显著低于IR3d组的(38.91±12.26) ng/ml,差异有统计学意义(P＜0.05);组织学评分结果,IR3d、5d组和HBO 3 d、5d组的组织学评分分别为(2.66±0.44)分、(3.20±0.53)分、(1.85±0.31)分和(2.29±0.32)分,均显著高于SH组的(0.38±0.10)分,差异均有统计学意义(P＜0.05),且HBO 3 d、5d组组织学评分均显著低于同时间点的IR组,差异均有统计学意义(P＜0.05).结论高压氧预处理可通过降低皮瓣移植术后大鼠IL-23水平来减轻皮瓣移植后缺血再灌注损伤的炎症反应.
목적 탐토고압양예처리대피판이식술후대서결혈재관주손상염증반응적영향.방법 장SD대서56지안수궤수자표법분위가수술조(SH,n=8),결혈재관주조(IR,n=24)화고압양예처리후결혈재관주조(HBO,n=24),IR조화HBO조재근거취혈적시간각분3개아조(IRld、3d、5d조화HBO 1 d、3d、5d조),매개아조8지대서.HBO 1 d、3d、5d조우조모전3d진행고압양예처리,매천2차(간격12 h),련속간예3d.7조균건립복부대체이식피판동물모형,SH조불협폐혹절단동정맥.조모성공후,SH조우술후제3천,기여6조칙우대응적시간점응용매련접면역흡부제측정(ELISA)법검측각조대서혈청IL-23수평;동시채용조직손상평분대SH조급IR3d、5d조화HBO 3 d、5d조적성활피판진행조직학평분.결과 경고압양예처리후,HBO 3 d조IL-23수평위(17.80±14.78) ng/ml,현저저우IR3d조적(38.91±12.26) ng/ml,차이유통계학의의(P＜0.05);조직학평분결과,IR3d、5d조화HBO 3 d、5d조적조직학평분분별위(2.66±0.44)분、(3.20±0.53)분、(1.85±0.31)분화(2.29±0.32)분,균현저고우SH조적(0.38±0.10)분,차이균유통계학의의(P＜0.05),차HBO 3 d、5d조조직학평분균현저저우동시간점적IR조,차이균유통계학의의(P＜0.05).결론 고압양예처리가통과강저피판이식술후대서IL-23수평래감경피판이식후결혈재관주손상적염증반응.
Objective To explore the effects of hyperbaric oxygen preconditioning on ischemia-reperfusion inflammatory reaction of rats after skin flap transplantation.Methods Fifty-six Sprague-Dawley rats were randomly divided into 3 groups:a sham ischemia-reperfusion (SH) group,an ischemia-reperfusion (IR) group and a hyperbaric oxygen reconditioning (HBO) group.Both IR group and HBO group were further divided into 3 subgroups,respectively,according to the time points of serum sampling for test post establishment of the IR model of the abdominal pedicle skin flap transplantation.The IR model of the abdominal pedicle skin flap transplantation was established in all the animals except those in the SH group,with those in the HBO group were preconditioned with HBO twice daily for 3 days before the operation.The blood was sampled at 1,3 and 5 day post-operation to test the level of IL-23 using enzyme-linked immunosorbeut assay (ELISA).The survival skin flaps were sampled from all the animals at 3 and 5 days after the operation for histological observation and evaluation.Results The average IL-23 level of HBO 3 d subgroup (17.80 ± 14.78) was significantly lower than that of the IR 3 d subgroup (38.91 ± 12.26).The average histological scores of the IR 3 d and 5 d subgroups,as well as HBO 3 d and 5 d subgroups were (2.66 ±0.44)and (3.2 ±0.53),(1.85 ±0.31) and (2.29 ±0.32),significantly higher than SH group (0.38 ±0.10).Moreover,the average histological score of the HBO 3 d and 5 d subgroups was significantly lower than IR 3 d and 5 d subgroups respectively.Conclusion Hyperbaric oxygen preconditioning can relieve the ischemia-reperfusion inflammatory reaction through reducing the serum level of IL-23 in rats after skin flap transplantation.