中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
Chinese Journal of Physical Medicine and Rehabilitation
2015年
8期
561-564
,共4页
孙国剑%马军廷%李占标%王强
孫國劍%馬軍廷%李佔標%王彊
손국검%마군정%리점표%왕강
运动训练%脑缺血再灌注%神经再生%神经生长因子%神经营养因子-3
運動訓練%腦缺血再灌註%神經再生%神經生長因子%神經營養因子-3
운동훈련%뇌결혈재관주%신경재생%신경생장인자%신경영양인자-3
Exercise training%Cerebral ischemia-reperfusion%Neural regeneration%Nerve growth factor%Neurotrophins-3
目的 观察游泳训练对脑梗死大鼠神经生长因子(NGF)及神经营养因子-3(NT-3)表达的影响,并初步探讨运动训练对脑梗死大鼠的神经保护机制.方法 共选取健康雄性SD大鼠45只,采用随机数字表法将其分为假手术组、对照组及训练组.采用线栓法将对照组及训练组大鼠制成左侧大脑中动脉阻塞(MCAO)2 h再灌注模型,假手术组大鼠制模期间不阻塞大脑中动脉血流.训练组大鼠制模后给予游泳训练,每日1次,每次持续10 min,对照组及假手术组大鼠制模后不给予任何特殊处理.于制模后3d、7d及14d时采用Bederson评分法评定各组大鼠神经功能缺损情况;采用RT-PCR法检测各组大鼠缺血侧脑皮质NGF及NT-3 mRNA表达量.结果 假手术组大鼠术后无神经行为缺陷,制模后3d、7d及14d时训练组大鼠Bederson评分均显著低于同时相点对照组水平(P＜0.05),高于同时相点假手术组水平(P＜0.05),并且制模后14 d时训练组Bederson评分[(1.20±0.45)分]亦显著低于制模后3d及7d时水平(P＜0.05).训练组各时相点缺血侧脑皮质NGF及NT-3 mRNA表达量均较对照组、假手术明显增强(P＜0.05);随着时间进展,制模后14d时训练组NGF及NT-3 mRNA含量[分别为(0.66±0.07),(0.79±0.06)]均较制模后3d及7d时明显提高(P＜0.05).结论 游泳训练能上调脑梗死大鼠缺血脑皮质NGF及NT-3 mRNA表达,这可能是运动训练促进脑梗死大鼠受损神经功能恢复的重要机制之一.
目的 觀察遊泳訓練對腦梗死大鼠神經生長因子(NGF)及神經營養因子-3(NT-3)錶達的影響,併初步探討運動訓練對腦梗死大鼠的神經保護機製.方法 共選取健康雄性SD大鼠45隻,採用隨機數字錶法將其分為假手術組、對照組及訓練組.採用線栓法將對照組及訓練組大鼠製成左側大腦中動脈阻塞(MCAO)2 h再灌註模型,假手術組大鼠製模期間不阻塞大腦中動脈血流.訓練組大鼠製模後給予遊泳訓練,每日1次,每次持續10 min,對照組及假手術組大鼠製模後不給予任何特殊處理.于製模後3d、7d及14d時採用Bederson評分法評定各組大鼠神經功能缺損情況;採用RT-PCR法檢測各組大鼠缺血側腦皮質NGF及NT-3 mRNA錶達量.結果 假手術組大鼠術後無神經行為缺陷,製模後3d、7d及14d時訓練組大鼠Bederson評分均顯著低于同時相點對照組水平(P＜0.05),高于同時相點假手術組水平(P＜0.05),併且製模後14 d時訓練組Bederson評分[(1.20±0.45)分]亦顯著低于製模後3d及7d時水平(P＜0.05).訓練組各時相點缺血側腦皮質NGF及NT-3 mRNA錶達量均較對照組、假手術明顯增彊(P＜0.05);隨著時間進展,製模後14d時訓練組NGF及NT-3 mRNA含量[分彆為(0.66±0.07),(0.79±0.06)]均較製模後3d及7d時明顯提高(P＜0.05).結論 遊泳訓練能上調腦梗死大鼠缺血腦皮質NGF及NT-3 mRNA錶達,這可能是運動訓練促進腦梗死大鼠受損神經功能恢複的重要機製之一.
목적 관찰유영훈련대뇌경사대서신경생장인자(NGF)급신경영양인자-3(NT-3)표체적영향,병초보탐토운동훈련대뇌경사대서적신경보호궤제.방법 공선취건강웅성SD대서45지,채용수궤수자표법장기분위가수술조、대조조급훈련조.채용선전법장대조조급훈련조대서제성좌측대뇌중동맥조새(MCAO)2 h재관주모형,가수술조대서제모기간불조새대뇌중동맥혈류.훈련조대서제모후급여유영훈련,매일1차,매차지속10 min,대조조급가수술조대서제모후불급여임하특수처리.우제모후3d、7d급14d시채용Bederson평분법평정각조대서신경공능결손정황;채용RT-PCR법검측각조대서결혈측뇌피질NGF급NT-3 mRNA표체량.결과 가수술조대서술후무신경행위결함,제모후3d、7d급14d시훈련조대서Bederson평분균현저저우동시상점대조조수평(P＜0.05),고우동시상점가수술조수평(P＜0.05),병차제모후14 d시훈련조Bederson평분[(1.20±0.45)분]역현저저우제모후3d급7d시수평(P＜0.05).훈련조각시상점결혈측뇌피질NGF급NT-3 mRNA표체량균교대조조、가수술명현증강(P＜0.05);수착시간진전,제모후14d시훈련조NGF급NT-3 mRNA함량[분별위(0.66±0.07),(0.79±0.06)]균교제모후3d급7d시명현제고(P＜0.05).결론 유영훈련능상조뇌경사대서결혈뇌피질NGF급NT-3 mRNA표체,저가능시운동훈련촉진뇌경사대서수손신경공능회복적중요궤제지일.
Objective To observe the influence of swimming training on the expression of nerve growth factor (NGF) and neurotrophins-3 (NT-3) in rats with cerebral infarction,and to explore the underlying neuroprotection mechanism of exercise training on cerebral infarction.Methods Forty-five healthy male Sprague-Dawley rats were randomly divided into a sham-operation group,a control group and a training group,with 15 rats in each group.Each group was further divided into a 3-day,7-day and 14-day subgroups,which amounts to 9 groups.To establish animal model of cerebral ischemia-reperfusion in rats,the intraluminal thread method was applied to cause left middle cerebral artery occlusion (MCAO) for 2 h before reperfusion.The rats of the training group were given swimming training for 10 min,once daily,while those of the sham-operation and control groups were not given any training.Neurological deficits were assessed using Bederson scores.The expression of NGF mRNA and NT-3 mRNA in the ischemiareperfusion pallium was examined using reverse transcription-polymerase chain reaction (RT-PCR).Results The rats of the sham-operation group showed no neurological deficits.At the same time points,the average Bederson scores of the training group were significantly lower than the control group,but significantly higher than the sham group.Moreover,the 14 d training group had the lowest Bederson score (1.20 ±0.45),compared to the value 3 and 7 days after modeling.The expression of NGF mRNA and NT-3 mRNA of ischemic cerebral cortex in the training group was significantly improved when compared to the sham-operation group or the control group.On day 14,the expression of the NGF mRNA (0.66 ± 0.07),and the NT-3 mRNA (0.79 ± 0.06),were significantly higher than those on day 3 and 7.Conclusions Swimming training could increase the expressions of NGF mRNA and NT-3 mRNA in the ischemic cerebral cortex.It might be one of the key mechanisms that exercise training could promote the recovery of damaged neurological function in rats with cerebral infarction.
