CAJ | 학술논문

目的观察当归挥发油对大鼠吗啡戒断体征及去甲肾上腺素神经递质释放的影响.方法 72只SD大鼠随机均分为空白组、模型组、可乐定组和当归挥发油高(80 mg/kg)、中(40 mg/kg)、低(20 mg/kg)剂量组.采用剂量递增法建立大鼠吗啡依赖模型,采用柳田知司评分法对大鼠戒断体征进行评分;采用酶联免疫吸附法榆测蓝斑核和前额叶皮层中去甲肾上腺素(NE)及其代谢产物间羟去甲肾上腺素(NMN)的含量.结果空白组、模型组、可乐定组和当归挥发油低、中、高剂量组戒断体征总评分值分别为(4.00±3.29)分、(30.13±4.41)分、(18.96±4.43)分、(26.04±4.13)分、(22.33±4.60)分和(19.00±3.47)分,当归挥发油呈剂量依赖地降低戒断体征总评分值(P＜0.01),同时显著性降低蓝斑核和前额叶皮层中NE及NMN的含量,降低NMN/NE值(P值均小于0.01).结论当归挥发油能够有效缓解大鼠吗啡戒断体征,其机制可能与抑制蓝斑核和前额叶皮层NE周转有关.
목적 관찰당귀휘발유대대서마배계단체정급거갑신상선소신경체질석방적영향.방법 72지SD대서수궤균분위공백조、모형조、가악정조화당귀휘발유고(80 mg/kg)、중(40 mg/kg)、저(20 mg/kg)제량조.채용제량체증법건립대서마배의뢰모형,채용류전지사평분법대대서계단체정진행평분;채용매련면역흡부법유측람반핵화전액협피층중거갑신상선소(NE)급기대사산물간간거갑신상선소(NMN)적함량.결과 공백조、모형조、가악정조화당귀휘발유저、중、고제량조계단체정총평분치분별위(4.00±3.29)분、(30.13±4.41)분、(18.96±4.43)분、(26.04±4.13)분、(22.33±4.60)분화(19.00±3.47)분,당귀휘발유정제량의뢰지강저계단체정총평분치(P＜0.01),동시현저성강저람반핵화전액협피층중NE급NMN적함량,강저NMN/NE치(P치균소우0.01).결론 당귀휘발유능구유효완해대서마배계단체정,기궤제가능여억제람반핵화전액협피층NE주전유관.
Objective To explore the effects of angelica naphtha on the withdrawal signs and norepinephrine neurotransmitter in uorphine-dependent rats.Methods 72 SD rats were randomly divided into control group,model group,clonidine group and three angelica naphtha groups (80,40 and 20 mg/kg).Rats were received gradualy increasing doses of morphine to produce physical dependence.Withdrawal symptoms were evaluated by Ryuta Tomoji score.The level of norepinephrine and normetanephrine(NMN) were tested by enzyme-linked immunosorbent assay(ELISA).Results The scores of withdrawal signs were (4.00± 3.29),(30.13±4.41),(18.96± 4.43),26.04±4.13),(22.33±4.60) and (19.00±3.47),respectively.The morphine withdrawal signs were reduced by angelica naphtha in a dose-dependent manner(P＜0.01),also significantly reduced the level of NE and NMN and the ratio of NMN/NE in the nucleus ceruleus and prefrontal cortex(all P＜0.01).Conclusion Angelica naphtha alleviates the withdrawal synlptons in morphine-dependent rats,which may be related to the inhibition of excessive turnover of norepinephrine neurotransmitters in the nucleus ceruleus and prefrontal cortex.