CAJ | 학술논문

目的探究采用神经微移植技术和传统移植技术将内侧神经节隆起细胞移植至亨廷顿病大鼠模型的疗效异同.方法 75只雌性成年SD大鼠,采用随机数字表法分为四组:对照组(ctrl,n=15)、假移植组(ST,n=20)、传统移植组(TT,n=20)和微移植组(MT,n=20),应用神经微移植技术(针头直径为50μm)和传统的移植技术(针头直径为470 μm)将神经节隆起细胞或磷酸缓冲盐溶液移植入单侧奎宁酸(Quinic acid,QA)损毁的大鼠纹状内,奎宁酸诱导损伤后及移植后第2,4,6,8,10,12周进行阿扑吗啡诱导的旋转试验和步移试验,移植12周后分析神经元核抗原(neuron specific nuclear protein,NeuN)、多巴胺、cAMP调节的磷蛋白(dopamine and adenosine 3 ' 5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达.结果在移植12周后,与TT组相比,MT组大鼠步移实验表现较好,TT组和MT组DARPP-32阳性细胞数目分别为(9 690±651)个和(13 194±976)个,差异具有统计学意义(P=0.020),TT组和MT组单侧移植物周围GFAP阳性细胞数目分别为(11 352±1 421)个和(6 558±694)个,差异具有统计学意义(P=0.002),TT组和MT组移植后存活率分别为55％和85％,差异具有统计学意义(P=0.012).结论微移植技术能提高内侧神经节隆起细胞移植治疗HD大鼠模型的效果,这些发现为HD的细胞移植治疗奠定了基础.
목적 탐구채용신경미이식기술화전통이식기술장내측신경절륭기세포이식지형정돈병대서모형적료효이동.방법 75지자성성년SD대서,채용수궤수자표법분위사조:대조조(ctrl,n=15)、가이식조(ST,n=20)、전통이식조(TT,n=20)화미이식조(MT,n=20),응용신경미이식기술(침두직경위50μm)화전통적이식기술(침두직경위470 μm)장신경절륭기세포혹린산완충염용액이식입단측규저산(Quinic acid,QA)손훼적대서문상내,규저산유도손상후급이식후제2,4,6,8,10,12주진행아복마배유도적선전시험화보이시험,이식12주후분석신경원핵항원(neuron specific nuclear protein,NeuN)、다파알、cAMP조절적린단백(dopamine and adenosine 3 ' 5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)、효질섬유산성단백(glial fibrillary acidic protein,GFAP)적표체.결과 재이식12주후,여TT조상비,MT조대서보이실험표현교호,TT조화MT조DARPP-32양성세포수목분별위(9 690±651)개화(13 194±976)개,차이구유통계학의의(P=0.020),TT조화MT조단측이식물주위GFAP양성세포수목분별위(11 352±1 421)개화(6 558±694)개,차이구유통계학의의(P=0.002),TT조화MT조이식후존활솔분별위55％화85％,차이구유통계학의의(P=0.012).결론 미이식기술능제고내측신경절륭기세포이식치료HD대서모형적효과,저사발현위HD적세포이식치료전정료기출.
Objective To compare the therapeutic effects of microtransplantation method and traditional transplantation method for transplanting ganglionic eminence cell into HD rats.Methods Seventy-five adult female Sprague-Dawley rats were randomly divided into four groups,control group (ctrl;n=15),sham transplantation group (ST;n=20),traditional transplantation group (TT;n=20),and microtransplantation group (MT;n =20).Ganglionic eminence cells or phosphate-buffered saline were transplanted into unilateral striatum of quinic acid lesion rats with micro-transplantation instruments (with an outer diameter of 50 μm) or traditional transplantation instruments (with an outer diameter of 470 μm).Apomorphine-induced rotation test and adjusting step test were assessed after QA-induced lesion and 2,4,6,8,10 and 12 weeks after transplantation.The expression of neuronal nuclei (NeuN),dopamine,cAMP-regulated phosphoprotein of molecular weight 32 kDa (DARPP-32),and glial fibrillary acidic protein (GFAP) were analyzed at 12 weeks after transplantation.Results Compared with traditional transplantation group,microtransplantation group performed well in stepping test.DARPP-32 positives cell in MT group (13 194±976) had a significantly larger number compared with TT group(9 690±651) (P=0.020).The TT group(11 352± 1 421) had a significantly larger number of GFAP-positive cells compared with MT groups (6 558 ± ±694) (P=0.002).The survival rate of the MT group(85％) during the 12 weeks after transplantation was significantly higher than that of the TT groups(55％) (P=0.012).Conclusion Micro-transplantation approach can minimize the trauma associated with transplantation and also provide accurate targets in the host brain.It may be an alternative transplantation strategy for Huntington's disease.