福建畜牧兽医
福建畜牧獸醫
복건축목수의
Journal of Animal Husbandry and Veterinary Medicine Fujian
2015年
5期
1-4
,共4页
猪蛔虫%各期虫体%半定量RT-PCR%基因表达
豬蛔蟲%各期蟲體%半定量RT-PCR%基因錶達
저회충%각기충체%반정량RT-PCR%기인표체
Ascaris sum%different stages of Ascaris suum%semi-quantitative RT-PCR%differentially expressed gene
采用半定量RT-PCR（Reverse Transcription-Polymerase Chain Reaction）方法，探讨编码铁蛋白基因（命名为2G04）在猪蛔虫不同发育期虫体的表达丰度。结果表明该基因在雄虫中没有检测到表达量，在其他各期幼虫均有较高表达，其中在虫卵中表达量最高。试验结果可为猪蛔虫的发育生物学和免疫防治奠定基础和提供科学依据。
採用半定量RT-PCR（Reverse Transcription-Polymerase Chain Reaction）方法，探討編碼鐵蛋白基因（命名為2G04）在豬蛔蟲不同髮育期蟲體的錶達豐度。結果錶明該基因在雄蟲中沒有檢測到錶達量，在其他各期幼蟲均有較高錶達，其中在蟲卵中錶達量最高。試驗結果可為豬蛔蟲的髮育生物學和免疫防治奠定基礎和提供科學依據。
채용반정량RT-PCR（Reverse Transcription-Polymerase Chain Reaction）방법，탐토편마철단백기인（명명위2G04）재저회충불동발육기충체적표체봉도。결과표명해기인재웅충중몰유검측도표체량，재기타각기유충균유교고표체，기중재충란중표체량최고。시험결과가위저회충적발육생물학화면역방치전정기출화제공과학의거。
This experiment adopted semiquantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)method to discuss the expression abundance of coding ferritin gene (named 2G04) in polypide in different developmental phases .The result showed that no expression was found in maleworm for this gene, and there have high expression in other different stages,among which the highest expression was found in eggs.The experimental result lays the foundation and provides the scientific basis for the developmental biolo-gy and immune control in Ascaris suum.
