江西医药
江西醫藥
강서의약
Jiangxi Medical Journal
2015年
9期
883-886
,共4页
肺纤维化%博莱霉素%葛根素%超氧化物歧化酶%丙二醛
肺纖維化%博萊黴素%葛根素%超氧化物歧化酶%丙二醛
폐섬유화%박래매소%갈근소%초양화물기화매%병이철
Pulmonary fibrosis%Bleomycin%Puerarin%SOD%MDA
目的：通过建立博莱霉素所诱导的大鼠肺间质纤维化模型，对肺纤维化模型大鼠病理形态、超氧化物歧化酶（SOD）、丙二醛（MDA）活性测定来探讨葛根素对实验性大鼠肺纤维化影响的作用机制。方法 SD雄性大鼠40只，随机分为对照组，模型组，葛根素组，激素组，每组10只，28d后，评价肺组织病理形态学变化，检测肺系数变化及各组肺组织匀浆中SOD活性和MDA含量。结果（1）各组肺系数比较：第7、28d，模型组肺系数较其他3组明显增加(P<0.01)，葛根素组与激素组肺系数比较无统计学意义(P>0.05)。（2）通过HE染色，观察到：葛根素与激素组的大鼠肺泡炎及肺纤维化程度均较模型组明显减轻，治疗7d后肺组织炎细胞、浸润区域较模型组明显减少；治疗28d后呈轻、中度肺纤维化改变，病变范围局限，肺泡间隔胶原纤维沉积呈束状。（3）葛根素与激素组较模型组大鼠肺组织匀浆SOD含量更高（P<0.01），MDA含量却降低（P<0.01），对照组大鼠肺组织匀浆SOD活性、MDA含量在各时间断基本一致。结论葛根素能减轻博莱霉素所致的大鼠肺泡炎及肺纤维化程度，提高肺组织SOD活力，降低MDA含量。
目的：通過建立博萊黴素所誘導的大鼠肺間質纖維化模型，對肺纖維化模型大鼠病理形態、超氧化物歧化酶（SOD）、丙二醛（MDA）活性測定來探討葛根素對實驗性大鼠肺纖維化影響的作用機製。方法 SD雄性大鼠40隻，隨機分為對照組，模型組，葛根素組，激素組，每組10隻，28d後，評價肺組織病理形態學變化，檢測肺繫數變化及各組肺組織勻漿中SOD活性和MDA含量。結果（1）各組肺繫數比較：第7、28d，模型組肺繫數較其他3組明顯增加(P<0.01)，葛根素組與激素組肺繫數比較無統計學意義(P>0.05)。（2）通過HE染色，觀察到：葛根素與激素組的大鼠肺泡炎及肺纖維化程度均較模型組明顯減輕，治療7d後肺組織炎細胞、浸潤區域較模型組明顯減少；治療28d後呈輕、中度肺纖維化改變，病變範圍跼限，肺泡間隔膠原纖維沉積呈束狀。（3）葛根素與激素組較模型組大鼠肺組織勻漿SOD含量更高（P<0.01），MDA含量卻降低（P<0.01），對照組大鼠肺組織勻漿SOD活性、MDA含量在各時間斷基本一緻。結論葛根素能減輕博萊黴素所緻的大鼠肺泡炎及肺纖維化程度，提高肺組織SOD活力，降低MDA含量。
목적：통과건립박래매소소유도적대서폐간질섬유화모형，대폐섬유화모형대서병리형태、초양화물기화매（SOD）、병이철（MDA）활성측정래탐토갈근소대실험성대서폐섬유화영향적작용궤제。방법 SD웅성대서40지，수궤분위대조조，모형조，갈근소조，격소조，매조10지，28d후，평개폐조직병리형태학변화，검측폐계수변화급각조폐조직균장중SOD활성화MDA함량。결과（1）각조폐계수비교：제7、28d，모형조폐계수교기타3조명현증가(P<0.01)，갈근소조여격소조폐계수비교무통계학의의(P>0.05)。（2）통과HE염색，관찰도：갈근소여격소조적대서폐포염급폐섬유화정도균교모형조명현감경，치료7d후폐조직염세포、침윤구역교모형조명현감소；치료28d후정경、중도폐섬유화개변，병변범위국한，폐포간격효원섬유침적정속상。（3）갈근소여격소조교모형조대서폐조직균장SOD함량경고（P<0.01），MDA함량각강저（P<0.01），대조조대서폐조직균장SOD활성、MDA함량재각시간단기본일치。결론갈근소능감경박래매소소치적대서폐포염급폐섬유화정도，제고폐조직SOD활력，강저MDA함량。
Objective To establish the Bleomycin-induced pulmonary fibrosis model in rats,explore the mechanism of the ef-fects of puerarin on the pulmonary fibrosis in rats by the histopathology,the activity of the SOD,the contents of MDA. Methods Forty male SD rats were divided into four groups:control group (n=10),model group (n=10),puerarin group (n=10) and glucocorti-coids group (n=10),On the 28th day,the lungs were harvested for histophological examintion,the contents of superoxide dismutase (SOD) and malonyldialde-hyde (MDA) were detected. Results The comparison of the lung coeffcients:The lung coeffieients in the model group and in the other group were significantly (P<0.01). Howevert there was no significance between puerarin group and glucocorticoids group(P>0.05). (2)By the HE staining the alveolar inflammation and the Pulmonary fibrosis were more mitigated ob-viously in puerarin and glucocorticoids groups than model group;after treating for 7 days,pulmonary inflammation cells and infil-tration area were more mitigated obviously in puerarin and glucocorticoids groups than in model Group;after treating for 28 days, the pulmonary fibrosis in puerarin and glucocorticoids group was changed lightly and moderately ,the collagen fibers in pleural membrane and alveolar membrane were bunehy. (3)The contents of SOD in lung tissues homogenate of puerarin and glucocorti-coids groups were higher than model group (P<0.01);but the contents of MDA in lung tissues homogenate of puerarin and gluco-corticoids groups were lower than model group(P<0.01);during each period,the activities of SOD and the contents of MDA in lung tissues homogenate of control group were consistent basically. Conclusion Puerarin could significantly alleviate the degree of the alveolitis and pulmonary fibrosis,increase activity of SOD in lungs the rats with pulmonary fibrosis,and lower the content of MDA.
