肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
Cancer Research and Clinic
2015年
9期
602-604,608
,共4页
朱其聪%毛高才%吕扬%王亚辉%杨琳%余书勇
硃其聰%毛高纔%呂颺%王亞輝%楊琳%餘書勇
주기총%모고재%려양%왕아휘%양림%여서용
前列腺肿瘤%骨唾液酸蛋白%前列腺特异性抗原%免疫组织化学
前列腺腫瘤%骨唾液痠蛋白%前列腺特異性抗原%免疫組織化學
전렬선종류%골타액산단백%전렬선특이성항원%면역조직화학
Prostatic neoplasms%Bone sialoprotein%Prostate-specific antigen%Immunohistochemistry
目的:探讨骨唾液酸蛋白(BSP)在前列腺癌组织中的表达及其意义。方法选取不同分级的前列腺癌组织(68例)和良性前列腺增生组织(22例),采用免疫组织化学SP法染色,检测BSP的表达情况。采用电化学发光法检测前列腺癌患者术前血清总前列腺特异性抗原(tPSA )的含量。结果大多数前列腺癌高表达BSP,阳性率为76.47%(52/68),高于前列腺增生组织的13.64%(3/22)(χ2=27.614,P<0.001),周边正常腺组织无或低表达BSP。68例前列腺癌组织按Gleason分级系统进行病理分级,分化良好、分化中等和分化不良癌组织中BSP的表达率分别为75.0%(12/16)、77.5%(31/40)和75.0%(9/12),差异无统计学意义(χ2=0.057,P=0.972)。病理分期pT2、pT3、pT4癌组织中BSP的表达率分别为62.2%(23/37)、95.2%(20/21)和90.0%(9/10),差异有统计学意义(χ2=9.338,P=0.009)。BSP阳性前列腺癌患者的血清tPSA含量[(69.06±25.52)μg/L]高于BSP阴性患者[(38.00±21.64)μg/L](F=19.355,P<0.001)。结论 BSP在前列腺癌中高表达,与病理分期、血清tPSA含量相关,可能与前列腺癌的生物学行为有关。
目的:探討骨唾液痠蛋白(BSP)在前列腺癌組織中的錶達及其意義。方法選取不同分級的前列腺癌組織(68例)和良性前列腺增生組織(22例),採用免疫組織化學SP法染色,檢測BSP的錶達情況。採用電化學髮光法檢測前列腺癌患者術前血清總前列腺特異性抗原(tPSA )的含量。結果大多數前列腺癌高錶達BSP,暘性率為76.47%(52/68),高于前列腺增生組織的13.64%(3/22)(χ2=27.614,P<0.001),週邊正常腺組織無或低錶達BSP。68例前列腺癌組織按Gleason分級繫統進行病理分級,分化良好、分化中等和分化不良癌組織中BSP的錶達率分彆為75.0%(12/16)、77.5%(31/40)和75.0%(9/12),差異無統計學意義(χ2=0.057,P=0.972)。病理分期pT2、pT3、pT4癌組織中BSP的錶達率分彆為62.2%(23/37)、95.2%(20/21)和90.0%(9/10),差異有統計學意義(χ2=9.338,P=0.009)。BSP暘性前列腺癌患者的血清tPSA含量[(69.06±25.52)μg/L]高于BSP陰性患者[(38.00±21.64)μg/L](F=19.355,P<0.001)。結論 BSP在前列腺癌中高錶達,與病理分期、血清tPSA含量相關,可能與前列腺癌的生物學行為有關。
목적:탐토골타액산단백(BSP)재전렬선암조직중적표체급기의의。방법선취불동분급적전렬선암조직(68례)화량성전렬선증생조직(22례),채용면역조직화학SP법염색,검측BSP적표체정황。채용전화학발광법검측전렬선암환자술전혈청총전렬선특이성항원(tPSA )적함량。결과대다수전렬선암고표체BSP,양성솔위76.47%(52/68),고우전렬선증생조직적13.64%(3/22)(χ2=27.614,P<0.001),주변정상선조직무혹저표체BSP。68례전렬선암조직안Gleason분급계통진행병리분급,분화량호、분화중등화분화불량암조직중BSP적표체솔분별위75.0%(12/16)、77.5%(31/40)화75.0%(9/12),차이무통계학의의(χ2=0.057,P=0.972)。병리분기pT2、pT3、pT4암조직중BSP적표체솔분별위62.2%(23/37)、95.2%(20/21)화90.0%(9/10),차이유통계학의의(χ2=9.338,P=0.009)。BSP양성전렬선암환자적혈청tPSA함량[(69.06±25.52)μg/L]고우BSP음성환자[(38.00±21.64)μg/L](F=19.355,P<0.001)。결론 BSP재전렬선암중고표체,여병리분기、혈청tPSA함량상관,가능여전렬선암적생물학행위유관。
Objective To investigate the expression of bone sialoprotein (BSP) in prostate cancer and its clinical significance. Methods Prostate cancer tissues of different pathological grades (68 cases) and benign prostatic hyperplasia tissues (22 cases) were selected. SP method was used to detect the expression of BSP. Serum total prostate-specific antigen (tPSA) levels of prostate cancer were detected by electrochemiluminescence immunoassay before the operation. Results Compared with no or low expression in the adjacent normal glandular tissues, the detectable levels of BSP were examined in most of the prostate cancer tissues. The expression rate of BSP in prostate cancer tissues was higher than that in benign prostatic hyperplasia tissues [76.47%(52/68) vs 13.64%(3/22),χ2=27.614, P<0.001]. The expression rates of BSP in well differentiated, moderately differentiated and poorly differentiated tissues according to cell differentiating degree (Gleason system) were 75.0 % (12/16), 77.5 % (31/40) and 75.0 % (9/12) respectively. There was no significant difference in various pathological grading (χ2=0.057, P=0.972). The expression rates of BSP in pathological stage pT2, pT3 and pT4 tissues were 62.16%(23/37), 95.24%(20/21) and 90.0%(9/10) respectively. A statistically significant association was found between BSP expression and pathological stage (χ2=9.338, P=0.009). Serum tPSA level of prostate cancer group with BSP expression was higher than that with no BSP expression [(69.06±25.52)μg/L vs (38.00±21.64)μg/L, F=19.355, P<0.001]. Conclusion The high expression of BSP in prostate cancer has a relationship with pathological stage and serum tPSA level, it may play an important role in the biological behaviour of prostate cancer.