肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
Cancer Research and Clinic
2015年
9期
589-592
,共4页
张玉敏%陈晖%马荣芬%刘艳红%李玉柱%张尊
張玉敏%陳暉%馬榮芬%劉豔紅%李玉柱%張尊
장옥민%진휘%마영분%류염홍%리옥주%장존
肺肿瘤%念珠菌属%纸片扩散抗菌试验%微量肉汤稀释法
肺腫瘤%唸珠菌屬%紙片擴散抗菌試驗%微量肉湯稀釋法
폐종류%념주균속%지편확산항균시험%미량육탕희석법
Lung neoplasms%Candida%Disk diffusion antimicrobial tests%Broth microdilution method
目的：与微量肉汤稀释法比较，评价丹麦ROSCO纸片扩散法在检测肺癌患者念珠菌药物敏感性方面的应用价值，为临床实验室寻找一种简便的念珠菌药物敏感性检测方法。方法采用丹麦ROSCO公司抗真菌药物敏感性纸片和法国生物梅里埃公司ATB FUNGUR2念珠菌药物敏感性板条检测肺癌患者感染的78株常见念珠菌对5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑4种抗真菌药物的敏感性。以美国临床实验室标准化委员会（NCCLS）M27-A方案的微量肉汤稀释法为金标准。结果以NCCLS微量肉汤稀释法作为金标准，纸片法检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑的药物敏感性的κ值达到了0.89，未出现一种方法敏感而另一种方法耐药的严重错误现象。用纸片扩散法对78株念珠菌的药物敏感性进行分析，各菌对5-氟胞嘧啶、两性霉素B敏感度高，分别为88.20%和89.17%，对氟康唑和伊曲康唑敏感度较低，分别为56.34%和52.12%。白色念珠菌和热带念珠菌对四种抗真菌药物的敏感度较高，分别为90.95%、85.71%，而光滑念珠菌和克柔念珠菌的敏感度低，分别为67.50%、41.67%。结论纸片扩散法与微量肉汤稀释法一致性高，在临床实验室可以替代微量肉汤稀释法进行念珠菌的药物敏感性分析。
目的：與微量肉湯稀釋法比較，評價丹麥ROSCO紙片擴散法在檢測肺癌患者唸珠菌藥物敏感性方麵的應用價值，為臨床實驗室尋找一種簡便的唸珠菌藥物敏感性檢測方法。方法採用丹麥ROSCO公司抗真菌藥物敏感性紙片和法國生物梅裏埃公司ATB FUNGUR2唸珠菌藥物敏感性闆條檢測肺癌患者感染的78株常見唸珠菌對5-氟胞嘧啶、兩性黴素B、氟康唑、伊麯康唑4種抗真菌藥物的敏感性。以美國臨床實驗室標準化委員會（NCCLS）M27-A方案的微量肉湯稀釋法為金標準。結果以NCCLS微量肉湯稀釋法作為金標準，紙片法檢測5-氟胞嘧啶、兩性黴素B、氟康唑、伊麯康唑的藥物敏感性的κ值達到瞭0.89，未齣現一種方法敏感而另一種方法耐藥的嚴重錯誤現象。用紙片擴散法對78株唸珠菌的藥物敏感性進行分析，各菌對5-氟胞嘧啶、兩性黴素B敏感度高，分彆為88.20%和89.17%，對氟康唑和伊麯康唑敏感度較低，分彆為56.34%和52.12%。白色唸珠菌和熱帶唸珠菌對四種抗真菌藥物的敏感度較高，分彆為90.95%、85.71%，而光滑唸珠菌和剋柔唸珠菌的敏感度低，分彆為67.50%、41.67%。結論紙片擴散法與微量肉湯稀釋法一緻性高，在臨床實驗室可以替代微量肉湯稀釋法進行唸珠菌的藥物敏感性分析。
목적：여미량육탕희석법비교，평개단맥ROSCO지편확산법재검측폐암환자념주균약물민감성방면적응용개치，위림상실험실심조일충간편적념주균약물민감성검측방법。방법채용단맥ROSCO공사항진균약물민감성지편화법국생물매리애공사ATB FUNGUR2념주균약물민감성판조검측폐암환자감염적78주상견념주균대5-불포밀정、량성매소B、불강서、이곡강서4충항진균약물적민감성。이미국림상실험실표준화위원회（NCCLS）M27-A방안적미량육탕희석법위금표준。결과이NCCLS미량육탕희석법작위금표준，지편법검측5-불포밀정、량성매소B、불강서、이곡강서적약물민감성적κ치체도료0.89，미출현일충방법민감이령일충방법내약적엄중착오현상。용지편확산법대78주념주균적약물민감성진행분석，각균대5-불포밀정、량성매소B민감도고，분별위88.20%화89.17%，대불강서화이곡강서민감도교저，분별위56.34%화52.12%。백색념주균화열대념주균대사충항진균약물적민감도교고，분별위90.95%、85.71%，이광활념주균화극유념주균적민감도저，분별위67.50%、41.67%。결론지편확산법여미량육탕희석법일치성고，재림상실험실가이체대미량육탕희석법진행념주균적약물민감성분석。
Objective To compare the ROSCO disk diffusion method with broth microdilution method (CLSI, M27-A) for antimicrobial susceptibility test of Candida species isolated from patients with lung cancer. Methods Danish ROSCO company disk diffusion testing method and bio Merieux ATB FUNGUR2 were applied to test 5-flucytosine, fluconazole, itraconazole and amphotericin B antimicrobial susceptibility for 78 Candida species strains isolated from patients with lung cancer. Results Through evaluating the susceptibility to 5-flucytosine, amphotericin B, fluconazole and itraconazole by disk diffusion method, the Kappa value was 0.89. The sensitive strains detected by one method did not show resistance in another method. The sensitive rates of 78 strains of Candida species to 5-flucytosine, amphotericin B, fluconazole and itraconazole were 88.20 %, 89.17 %, 56.34 % and 52.12 %. The susceptibility of C.albicans, C.tropicalis, C.glabrata and C.krusei to four kinds of antifungal agents was 90.95 %, 85.71 %, 67.50 % and 41.67 %respectively. Conclusions Results of disk diffusion method coincide well with broth microdilution method. It can be chosen as a clinical routine method for antimicrobial susceptibility test.
