CAJ | 학술논문

目的：建立一种用抑制电导离子色谱仪测定实验动物环境空气中氨的方法。方法以稀硫酸作为吸收液采集空气中的氨，使氨在吸收液中转化为铵离子，选用抑制型电导检测器， CS12 A阳离子分析柱，以甲磺酸作为淋洗液，通过测定吸收液中的铵离子的含量来计算空气中氨的浓度。结果铵离子在0～1．00μg／mL范围内具有良好的线性（ r＝0．9991），方法精密度高（ RSD＜6．38％），最低检出浓度为0．012 mg／m3，方法的平均回收率98．8％。结论该法操作简便，无干扰离子影响，可作为实验动物环境空气中氨的测定方法。
목적：건립일충용억제전도리자색보의측정실험동물배경공기중안적방법。방법이희류산작위흡수액채집공기중적안，사안재흡수액중전화위안리자，선용억제형전도검측기， CS12 A양리자분석주，이갑광산작위림세액，통과측정흡수액중적안리자적함량래계산공기중안적농도。결과안리자재0～1．00μg／mL범위내구유량호적선성（ r＝0．9991），방법정밀도고（ RSD＜6．38％），최저검출농도위0．012 mg／m3，방법적평균회수솔98．8％。결론해법조작간편，무간우리자영향，가작위실험동물배경공기중안적측정방법。
ObjectiveToestablishanionchromatography(IC)methodfordetectionofammoniainthe environmental air of laboratory animal facilities .Methods Dilute sulfuric acid was used as the absorption liquid to collect ammonia in the air.Ammonia was converted into ammonium ion in the absorption solution .With methylene sulfonic acid as the pouring liquid , the content of ammonium ion in the absorption solution was detected , and the concentration of ammonia in the air was calculated.Results The ammonium ion showed good linearity in the range of 0 to 1μg/mL (r=0.9994), and high precision ( RSD<6.38%) .The lowest detected concentration was 0.012 mg/m3 , and the average recovery was 98.8%.Conclusions This is a simple method , easy to operate , not interfered by other ions , and can be used to detect the ammonia in the environmental air of laboratory animal facilities .