CAJ | 학술논문

目的：通过比较4种不同剂量、周期的环磷酰胺建立小鼠免疫力低下模型，并采用偏最小二乘法（ PLS）建模分析筛选出最优方案。方法雄性昆明小鼠58只随机分成5组，分别为正常组10只，每日注射生理盐水；模型1组12只，按照40 mg／kg的剂量连续注射环磷酰胺溶液10 d；模型2组12只，按照80 mg／kg的剂量连续注射环磷酰胺溶液3 d；模型3组12只按照40 mg／kg的剂量注射2次环磷酰胺溶液，每次间隔（2～3）d；模型4组12只按照50 mg／kg的剂量连续注射环磷酰胺溶液7 d。监测不同剂量和不同周期给予小鼠环磷酰胺后，模型小鼠的饮食、饮水、体重等日常代谢指标的变化；测定小鼠胸腺、脾等脏器指数以及血常规等免疫指标的变化；采用SIMCA－P软件处理小鼠的终体重、终肛温、脏器指数、血常规等指标，应用偏最小二乘法（ PLS）综合评价免疫力低下小鼠模型的建立。结果与正常组相比，模型组小鼠体重和肛温降低且差别有显著性意义（ P＜0．05）；日平均饮食、饮水量降低且差异有显著性（ P＜0．01）；脾指数和胸腺指数降低且差别有极显著性意义（ P＜0．01）；模型组嗜碱细胞绝对值和嗜碱细胞百分比均降低且差异有显著性（ P＜0．05），模型组平均RBC血红蛋白浓度、大血小板比率均升高且有显著性差异，模型1组、模型2组、模型4组的白细胞、淋巴细胞绝对值均降低且差异有显著性（P＜0．05）。 PLS分析处理表明，模型1组、模型2组、模型4组与正常组比较差异都具有显著性（P＜0．01），其中模型1组差异最大。结论通过PLS分析方法综合不同指标，模型1组按照40 mg／kg的剂量连续注射10 d为最佳造模方法，为建立稳定的免疫力低下模型提供实验依据。
목적：통과비교4충불동제량、주기적배린선알건립소서면역력저하모형，병채용편최소이승법（ PLS）건모분석사선출최우방안。방법웅성곤명소서58지수궤분성5조，분별위정상조10지，매일주사생리염수；모형1조12지，안조40 mg／kg적제량련속주사배린선알용액10 d；모형2조12지，안조80 mg／kg적제량련속주사배린선알용액3 d；모형3조12지안조40 mg／kg적제량주사2차배린선알용액，매차간격（2～3）d；모형4조12지안조50 mg／kg적제량련속주사배린선알용액7 d。감측불동제량화불동주기급여소서배린선알후，모형소서적음식、음수、체중등일상대사지표적변화；측정소서흉선、비등장기지수이급혈상규등면역지표적변화；채용SIMCA－P연건처리소서적종체중、종항온、장기지수、혈상규등지표，응용편최소이승법（ PLS）종합평개면역력저하소서모형적건립。결과여정상조상비，모형조소서체중화항온강저차차별유현저성의의（ P＜0．05）；일평균음식、음수량강저차차이유현저성（ P＜0．01）；비지수화흉선지수강저차차별유겁현저성의의（ P＜0．01）；모형조기감세포절대치화기감세포백분비균강저차차이유현저성（ P＜0．05），모형조평균RBC혈홍단백농도、대혈소판비솔균승고차유현저성차이，모형1조、모형2조、모형4조적백세포、림파세포절대치균강저차차이유현저성（P＜0．05）。 PLS분석처리표명，모형1조、모형2조、모형4조여정상조비교차이도구유현저성（P＜0．01），기중모형1조차이최대。결론통과PLS분석방법종합불동지표，모형1조안조40 mg／kg적제량련속주사10 d위최가조모방법，위건립은정적면역력저하모형제공실험의거。
ObjectiveComparingfourgroupsofimmunosuppressivemousemodelsestablishedby cyclophosphamide administration in different doses and periods , we used partial least squares ( PLS) analysis to evaluate the immunosuppressive mouse models comprehensively and select the appropriate way to establish this model .Methods In this experimental study, 58 male KM mice were randomly divided into five groups: normal group (10 mice) were given normal saline daily by i.p.injection, model group 1 (12 mice) was given 40 mg/kg CTX daily by i.p.injection for 10 days, model group 2 (12 mice) was given 80 mg/kg CTX daily by i.p.injection for 3 days, model group 3 (12 mice) was given 40 mg/kg CTX daily by i.p.injection twice for a week, model group 4 (12 mice) was given 50 mg/kg CTX daily by i.p.injection for 7 days.After the injection of cyclophosphamide , the daily metabolic activities were detected everyday such as body weight , water intake , and food intake , organ index and immunological indexes such as blood RT of the model mice were measured as well .Using partial least squares ( PLS) analysis to the models and analyzing the final weight , final anal temperature , organ index , and blood routine examination in order to evaluate the immunosuppressive mouse models comprehensively .Results Compared with the normal group , different dosages of CTX reduced the weight and anal temperature of model mice (P<0.05), the food intake and water intake (P<0.01), and the spleen index and thymus index ( P<0.01 ) .Besides , the amount and percentage of basophilic granulocytes decreased ( P <0.05 ) , and the percentage of MCHC , macrophage went up , as well as the absolute value of WBC and lymphocytes were decreased in the model groups 1, 2, and 4 (P<0.05).According to the PLS analysis, there were significant differences among models 1, 2, and 4 when compared with the normal group , especially the most different in the model group 1.Conclusions After the PLS analysis of different indexes , the optimal way to establish immunosuppressive mouse models is the procedure with 40 mg/kg CTX daily injected i .p.for ten days .Our findings provide experimental evidence for the establishment of immunosuppressive mouse models .