CAJ | 학술논문

目的：研究高压氧（HBO）对盐酸二甲双胍（MET）药代动力学的影响。方法200只小鼠随机分成对照组104只，和高压氧组（HBO组）96只，2组均按150 mg·kg-1剂量单次灌服盐酸二甲双胍溶液。HBO组灌服后立即置于高压氧舱，稳压（0.20 MPa）吸纯氧20 min，升压、减压各5 min；对照组仅单次灌服盐酸二甲双胍溶液。2组在给药前及给药后不同时间点各取8只小鼠，球后静脉丛取血，采用高效液相色谱法测定血浆中MET的浓度，计算药代动力学参数。结果HBO组峰浓度（Cmax）、达峰时间（tmax）、血浆半衰期（t1/2）、平均血药浓度一时间曲线下面积（AUC）、清除率（CL/F）和表观分布容积（Vd）分别为：（9.64±2.94）μg·mL-1、（0.42±0.12）h、（5.28±0.78）h、（29.76±5.21）μg·h·mL-1、（4.94±0.65）L·h-1·kg-1、（12.46±3.17）L·kg-1；对照组分别为：（18.68±4.26）μg·mL-1、（0.10±0.04）h、（7.41±1.06）h、（44.12±7.15）μg·h·mL-1、（3.32±0.63）L·h-1·kg-1、（7.62±1.97）L·kg-1。结论HBO可以明显加快小鼠体内MET的消除，对药代动力学参数有明显的影响。
목적：연구고압양（HBO）대염산이갑쌍고（MET）약대동역학적영향。방법200지소서수궤분성대조조104지，화고압양조（HBO조）96지，2조균안150 mg·kg-1제량단차관복염산이갑쌍고용액。HBO조관복후립즉치우고압양창，은압（0.20 MPa）흡순양20 min，승압、감압각5 min；대조조부단차관복염산이갑쌍고용액。2조재급약전급급약후불동시간점각취8지소서，구후정맥총취혈，채용고효액상색보법측정혈장중MET적농도，계산약대동역학삼수。결과HBO조봉농도（Cmax）、체봉시간（tmax）、혈장반쇠기（t1/2）、평균혈약농도일시간곡선하면적（AUC）、청제솔（CL/F）화표관분포용적（Vd）분별위：（9.64±2.94）μg·mL-1、（0.42±0.12）h、（5.28±0.78）h、（29.76±5.21）μg·h·mL-1、（4.94±0.65）L·h-1·kg-1、（12.46±3.17）L·kg-1；대조조분별위：（18.68±4.26）μg·mL-1、（0.10±0.04）h、（7.41±1.06）h、（44.12±7.15）μg·h·mL-1、（3.32±0.63）L·h-1·kg-1、（7.62±1.97）L·kg-1。결론HBO가이명현가쾌소서체내MET적소제，대약대동역학삼수유명현적영향。
Objective To investigate the effects ofhyperbaric oxygen(HBO)on the pharmacokinetics of MET.Methods Two hundred mice were randomly divided into 2 groups, the HBO group and the control group. The 2 groups of animals received gavage of MET with a single-dose of 150 mg·kg-1. The animals in the HBO group were immediately put into the hyperbaric oxygen chamber and maintained at a pressure of 0.20 MPa for a duration of 20 min. The time used for compression and decompression was both 5 min. The animals in the control group were just given a single gavage of MET. Eight animals from each group were chosen at different time points both before and after drug admin-istration, for collection of blood samples from the eyeballs. Concentrations of MET in serum were monitored with high performance liquid chromatography, and pharmacokinetic indices were calculated accordingly. Results The main pharmaeokinetic parameters of the HBO group were as follows:Cmax (9.64±2.94)μg·mL-1, tmax (0.42±0.12)h, t1/2 (5.28±0.78)h, AUC(29.76±5.21)μg·mL-1·h, CL/F (4.94±0.65)L·h-1·kg-1, Vd (12.46±3.17)L·kg-1, while the corresponding results of the control group were Cmax (18.68±4.26)μg·mL-1, tmax (0.10±0.04)h, t1/2 (7.41±1.06)h, AUC(44.12± 7.15)μg·mL-1·h, CL/F (3.32±0.63)L·h-1·kg-1, Vd (7.62±1.97)L·kg-1. Conclusion HBO could obviously acceler-ate the elimination of MET and seemed to have significant effect on pharmacokinetic parameters.