CAJ | 학술논문

目的：比较分析Dunkin Hartley白化豚鼠与Hartley花色豚鼠在构建高脂血症模型以及评价降脂药效学方面的差异，为高脂血症模型建立时豚鼠的品种选择提供参考。方法选取5周龄的白化豚鼠和花色豚鼠测定基础血脂水平，具体包括总胆固醇（ TC）、甘油三酯（ TG）、高密度脂蛋白（ HDL－C）和低密度脂蛋白（ LDL－C）。随后分别随机分为正常组（12只）和高脂模型组（24只），正常组饲喂普通饲料，高脂模型组饲喂高脂饲料以构建高脂血症模型；饲喂4周后，同上测定血脂水平。进一步将高脂模型组随机分为匹伐他汀钙组（12只）和溶媒对照组（12只），匹伐他汀钙组每天灌胃给予匹伐他汀钙（1mg／kg），溶媒对照组每天灌胃给予同体积的溶媒，持续给药4周，并同步饲喂高脂饲料；给药结束后，同上测定血脂水平。结果当给予普通饲料喂养时，白化豚鼠血脂较为稳定，随周龄增长没有显著变化，而花色豚鼠的TC和LDL－C在9周龄后才比较稳定。当给予高脂饲料喂养时，白化豚鼠的TC、TG和LDL－C在造模的4周时间内迅速上升，且增幅显著大于花色豚鼠，HDL－C与花色豚鼠的变化幅度相似。运用模型组豚鼠，进行降脂药效学的考察，匹伐他汀钙在白化豚鼠高脂血症模型中降脂（ TC、TG和LDL－C）幅度更大、升高HDL－C的作用更显著。结论在选用正常豚鼠评价降脂作用时，降TG或升HDL－C类药物宜选用5周龄之后的白化豚鼠，降TC或LDL－C类药物宜选用9周龄之后的花色豚鼠；在选用高脂血症豚鼠模型评价降脂作用时，白化豚鼠较花色豚鼠在造模后的血脂水平更加紊乱，且对降脂药物的敏感程度更高，故可作为构建高脂血症模型和评价降脂药效学研究较佳的实验动物。
목적：비교분석Dunkin Hartley백화돈서여Hartley화색돈서재구건고지혈증모형이급평개강지약효학방면적차이，위고지혈증모형건립시돈서적품충선택제공삼고。방법선취5주령적백화돈서화화색돈서측정기출혈지수평，구체포괄총담고순（ TC）、감유삼지（ TG）、고밀도지단백（ HDL－C）화저밀도지단백（ LDL－C）。수후분별수궤분위정상조（12지）화고지모형조（24지），정상조사위보통사료，고지모형조사위고지사료이구건고지혈증모형；사위4주후，동상측정혈지수평。진일보장고지모형조수궤분위필벌타정개조（12지）화용매대조조（12지），필벌타정개조매천관위급여필벌타정개（1mg／kg），용매대조조매천관위급여동체적적용매，지속급약4주，병동보사위고지사료；급약결속후，동상측정혈지수평。결과당급여보통사료위양시，백화돈서혈지교위은정，수주령증장몰유현저변화，이화색돈서적TC화LDL－C재9주령후재비교은정。당급여고지사료위양시，백화돈서적TC、TG화LDL－C재조모적4주시간내신속상승，차증폭현저대우화색돈서，HDL－C여화색돈서적변화폭도상사。운용모형조돈서，진행강지약효학적고찰，필벌타정개재백화돈서고지혈증모형중강지（ TC、TG화LDL－C）폭도경대、승고HDL－C적작용경현저。결론재선용정상돈서평개강지작용시，강TG혹승HDL－C류약물의선용5주령지후적백화돈서，강TC혹LDL－C류약물의선용9주령지후적화색돈서；재선용고지혈증돈서모형평개강지작용시，백화돈서교화색돈서재조모후적혈지수평경가문란，차대강지약물적민감정도경고，고가작위구건고지혈증모형화평개강지약효학연구교가적실험동물。
ObjectiveTocomparethedifferencesoftwostocksofguineapigs,thealbinoguineapigsandpigment guinea pigs , in establishing dyslipidemic model , to evaluate their lipid-lowering action , and to compare their properties for development of hyperlipidemia .Methods Two stocks of the 5-week-old guinea pigs were randomly divided into two groups, normal group (NC) and model group (Model).For the NC group, 12 guinea pigs were fed with normal chew .For the model group , after fed with high-fat diet for four weeks , 24 male guinea pigs were randomly grouped and treated with vehicle (VC group) and pitavastatin (Pit group) calcium, respectively, by gavage as well as received high-fat diet.Before and after modeling and pitavastatin treatment , blood samples were collected and subjected to analysis of plasma TC , TG, HDL-C and LDL-C, respectively .Results In the normal group , the blood lipid levels of albino guinea pigs were more stable than that of the pigmented pigs with the increase of age .After fed with high-fat diet , the plasma lipid levels of TC , TG and LDL-C were significantly increased in the two strains of guinea pigs , while HDL-C showed a decrease to varying degrees .Interestingly , the lipid level in the albino guinea pigs was significantly higher than that of pigment guinea pigs . And also, after drug administration for four weeks , pitavastatin treatment significantly decreased the elevated lipid level of TC, TG and LDL-C in the albino guinea pigs compared with that in the pigment guinea pigs .Conclusions The albino guinea pigs and pigment guinea pigs demonstrate certain differences in establishing dyslipidemic model and evaluating lipid -lowering pharmacodynamics .However , compared with the pigment guinea pigs , the albino guinea pigs have obvious superiority because of easy establishment of hyperlipidemia model and are more sensitive to lipid -lowering drugs .