CAJ | 학술논문

目的：探讨不同体积的自体血液致大鼠硬膜下血肿模型稳定性的影响。方法将大鼠随机分为假手术组和300μL、500μL、700μL（即分别向硬膜下注射300μL、500μL、700μL的自体血液）模型组。分别于术后第2、4、6、8、10、14天，每组各取6只，以腹主动脉取血，取脑组织，并采用酶联免疫吸附（ELISA）方法测定各组大鼠血浆中NSE、S100B蛋白的含量。结果与假手术组比较，300μL组血液的NSE在第2、4天时显著增加（ P＜0．01），在第6、8天时渐渐减少（P＜0．05），说明血肿开始消散，在第10、14天时恢复到接近假手术组（P＞0．05）；500μL组和700μL组的NSE含量在第2、6、8、10、14天时都显著升高（P＜0．01），而在第4天时升高不明显（P＞0．05）。300μL组血液的S100B蛋白含量在第4天时较高（P＜0．01），在第2、6天时较低（P＜0．05），在第8、10、14天时含量接近假手术组（P＞0．05），说明血肿自行消散，损伤自行修复；500μL组和700μL组的S100B蛋白含量一直处于较高的状态（ P＜0．05）。结论相对于300μL组和700μL组，500μL组下的大鼠急性硬膜下血肿模型稳定性更好，能够更好的应用于大鼠急性硬膜下血肿的实验研究。
목적：탐토불동체적적자체혈액치대서경막하혈종모형은정성적영향。방법장대서수궤분위가수술조화300μL、500μL、700μL（즉분별향경막하주사300μL、500μL、700μL적자체혈액）모형조。분별우술후제2、4、6、8、10、14천，매조각취6지，이복주동맥취혈，취뇌조직，병채용매련면역흡부（ELISA）방법측정각조대서혈장중NSE、S100B단백적함량。결과여가수술조비교，300μL조혈액적NSE재제2、4천시현저증가（ P＜0．01），재제6、8천시점점감소（P＜0．05），설명혈종개시소산，재제10、14천시회복도접근가수술조（P＞0．05）；500μL조화700μL조적NSE함량재제2、6、8、10、14천시도현저승고（P＜0．01），이재제4천시승고불명현（P＞0．05）。300μL조혈액적S100B단백함량재제4천시교고（P＜0．01），재제2、6천시교저（P＜0．05），재제8、10、14천시함량접근가수술조（P＞0．05），설명혈종자행소산，손상자행수복；500μL조화700μL조적S100B단백함량일직처우교고적상태（ P＜0．05）。결론상대우300μL조화700μL조，500μL조하적대서급성경막하혈종모형은정성경호，능구경호적응용우대서급성경막하혈종적실험연구。
ObjectiveToexplorethestabilityofratmodelsofsubduralhematomapreparedbysubduralinjection of different volumes of autologous blood .Methods The rats were randomly divided into sham group (36), 300μL blood group, 500 μL blood group, and 700 μL blood group (each group 60 rats).The rats of model groups received subdural injection of 300 μL, 500 μL, or 700 μL autologous blood, respectively.At the postoperative 2nd, 4th, 6th, 8th, 10th, 14th days, blood samples were taken from the abnormal aorta , and the brains were taken out for gross examination and taking photographs , six rats were used for each time .Enzyme linked immunosorbent assay ( ELISA ) was performed to determine the content of serum NSE and S100B proteins in the rats in each group.Results Compared with the sham operation group, the serum NSE in the 300μL group was significantly increased at the 2nd and 4th days (P<0.01), and then gradually reduced at the 6th and 8th days (P<0.05), indicating that the hematoma began to disappear , and at the 10th and 14th days returned to a similar level of the sham operation group (P>0.05).In the 500 μL and 700 μL blood groups, the NSE contents at 2nd, 6th, 8th, 10th and 14th days were significantly increased ( P <0.01 ), but not significantly increased at the 4th day ( P >0.05 ).The content of S100B protein in the 300 μL blood group was significantly higher at the fourth day (P<0.01), lower at the 2nd and 6th days (P<0.05), and at 8th, 10th and 14th days was similar to that in the sham operation group ( P >0.05 for all ) , indicating that the hematoma disappeared gradually, and the damages repaired .The S100B protein content of the 500 μL and 700 μL blood groups was constantly kept at a higher level ( P<0.05 ) .Conclusions Compared with the 300 μL ad 700 μL blood groups , the rat model of subdural hematoma developed by subdural injection of 500 μL autologous blood is the best , and can be used for studies of rat subdural hematoma .