CAJ | 학술논문

目的：探讨肥胖与雌性幼龄SD大鼠与青春发育提前之间的相关性。方法将10只雌性SD大鼠与6只雄性SD大鼠随机分为两组，分别为亲代模型组（雌鼠n＝5，雄鼠n＝3）、亲代对照组（雌鼠n＝5，雄鼠n＝3）。模型组以高脂饲料饲喂8周造模，对照组用普通大鼠饲料饲喂，8周后筛选体质量大于对照组20％的模型组亲代大鼠体进行交配，繁殖的雌性仔鼠设定为模型组仔鼠，亲代对照组大鼠繁殖的雌性仔鼠作为对照组仔鼠。模型组仔鼠造模方法与模型组亲代大鼠相同，均为同配方高脂饲料饲喂，21日龄断乳后饲喂5周，对照组仔鼠则用普通饲料饲喂5周。自21日龄起每日观察仔鼠阴门开启情况，阴门开启后每日均进行阴道涂片检查，观察动情周期，并称量体重；体长及腹围的测量每两周进行1次；两组实验仔鼠均在56日龄时结束实验，腹主动脉取血，检测两组幼鼠56日龄血清胆固醇（CH）、甘油三酯（TG）、血清卵泡刺激素（FSH）、促黄体生成素（LH）、雌二醇（E2）。实验结束后解剖56日龄仔鼠，取材子宫、卵巢，计算卵巢指数、子宫指数，子宫、卵巢做病理切片HE染色，计数卵巢最大横截面黄体个数和成熟卵个泡数。结果①56日龄造模结束，模型组幼鼠体重、腹围均高于对照组幼鼠，P＜0．001；②模型组幼鼠血清TG、CH均高于对照组，P＜0．001；③模型组幼鼠血清LH、E2浓度高于对照组幼鼠，P＜0．001，血清FSH浓度低于对照组，P＜0．001；④模型组幼鼠卵巢病理切片中最大横截面黄体计数较对照组多，P＜0．001；成熟卵泡计数少于对照组，P＜0．001；⑤Pearson相关性分析结果为：幼龄SD大鼠体质量与第一次动情间期、阴门开启时间两项指标呈成负相关，与血清LH、卵巢最大横截面切片黄体计数两项指标成正相关；幼龄SD大鼠腹围与第一次动情间期出现时间、阴门开启时间两项指标成负相关，与血清LH、卵巢最大横截面切片黄体计数两项指标成正相关。结论幼龄SD大鼠青春发育评判指标与肥胖指标具有相关性，呈正相关。即具有肥胖特征的幼龄SD大鼠青春发育较之正常对照组幼鼠提前。目的：探討肥胖與雌性幼齡SD大鼠與青春髮育提前之間的相關性。方法將10隻雌性SD大鼠與6隻雄性SD大鼠隨機分為兩組，分彆為親代模型組（雌鼠n＝5，雄鼠n＝3）、親代對照組（雌鼠n＝5，雄鼠n＝3）。模型組以高脂飼料飼餵8週造模，對照組用普通大鼠飼料飼餵，8週後篩選體質量大于對照組20％的模型組親代大鼠體進行交配，繁殖的雌性仔鼠設定為模型組仔鼠，親代對照組大鼠繁殖的雌性仔鼠作為對照組仔鼠。模型組仔鼠造模方法與模型組親代大鼠相同，均為同配方高脂飼料飼餵，21日齡斷乳後飼餵5週，對照組仔鼠則用普通飼料飼餵5週。自21日齡起每日觀察仔鼠陰門開啟情況，陰門開啟後每日均進行陰道塗片檢查，觀察動情週期，併稱量體重；體長及腹圍的測量每兩週進行1次；兩組實驗仔鼠均在56日齡時結束實驗，腹主動脈取血，檢測兩組幼鼠56日齡血清膽固醇（CH）、甘油三酯（TG）、血清卵泡刺激素（FSH）、促黃體生成素（LH）、雌二醇（E2）。實驗結束後解剖56日齡仔鼠，取材子宮、卵巢，計算卵巢指數、子宮指數，子宮、卵巢做病理切片HE染色，計數卵巢最大橫截麵黃體箇數和成熟卵箇泡數。結果①56日齡造模結束，模型組幼鼠體重、腹圍均高于對照組幼鼠，P＜0．001；②模型組幼鼠血清TG、CH均高于對照組，P＜0．001；③模型組幼鼠血清LH、E2濃度高于對照組幼鼠，P＜0．001，血清FSH濃度低于對照組，P＜0．001；④模型組幼鼠卵巢病理切片中最大橫截麵黃體計數較對照組多，P＜0．001；成熟卵泡計數少于對照組，P＜0．001；⑤Pearson相關性分析結果為：幼齡SD大鼠體質量與第一次動情間期、陰門開啟時間兩項指標呈成負相關，與血清LH、卵巢最大橫截麵切片黃體計數兩項指標成正相關；幼齡SD大鼠腹圍與第一次動情間期齣現時間、陰門開啟時間兩項指標成負相關，與血清LH、卵巢最大橫截麵切片黃體計數兩項指標成正相關。結論幼齡SD大鼠青春髮育評判指標與肥胖指標具有相關性，呈正相關。即具有肥胖特徵的幼齡SD大鼠青春髮育較之正常對照組幼鼠提前。
목적：탐토비반여자성유령SD대서여청춘발육제전지간적상관성。방법장10지자성SD대서여6지웅성SD대서수궤분위량조，분별위친대모형조（자서n＝5，웅서n＝3）、친대대조조（자서n＝5，웅서n＝3）。모형조이고지사료사위8주조모，대조조용보통대서사료사위，8주후사선체질량대우대조조20％적모형조친대대서체진행교배，번식적자성자서설정위모형조자서，친대대조조대서번식적자성자서작위대조조자서。모형조자서조모방법여모형조친대대서상동，균위동배방고지사료사위，21일령단유후사위5주，대조조자서칙용보통사료사위5주。자21일령기매일관찰자서음문개계정황，음문개계후매일균진행음도도편검사，관찰동정주기，병칭량체중；체장급복위적측량매량주진행1차；량조실험자서균재56일령시결속실험，복주동맥취혈，검측량조유서56일령혈청담고순（CH）、감유삼지（TG）、혈청란포자격소（FSH）、촉황체생성소（LH）、자이순（E2）。실험결속후해부56일령자서，취재자궁、란소，계산란소지수、자궁지수，자궁、란소주병리절편HE염색，계수란소최대횡절면황체개수화성숙란개포수。결과①56일령조모결속，모형조유서체중、복위균고우대조조유서，P＜0．001；②모형조유서혈청TG、CH균고우대조조，P＜0．001；③모형조유서혈청LH、E2농도고우대조조유서，P＜0．001，혈청FSH농도저우대조조，P＜0．001；④모형조유서란소병리절편중최대횡절면황체계수교대조조다，P＜0．001；성숙란포계수소우대조조，P＜0．001；⑤Pearson상관성분석결과위：유령SD대서체질량여제일차동정간기、음문개계시간량항지표정성부상관，여혈청LH、란소최대횡절면절편황체계수량항지표성정상관；유령SD대서복위여제일차동정간기출현시간、음문개계시간량항지표성부상관，여혈청LH、란소최대횡절면절편황체계수량항지표성정상관。결론유령SD대서청춘발육평판지표여비반지표구유상관성，정정상관。즉구유비반특정적유령SD대서청춘발육교지정상대조조유서제전。
ObjectiveToexplorethecorrelationofobesityandearlypubertyinimmaturefemaleSDratsby comparison of puberty development-related indicators between the model group and control group after setting up SD immature rat models of nutritional obesity .Methods Ten female rats and 6 male rats were divided in two groups: the parent model group (5 females and 3males) and parent control group (5 females and 3 males).The model group was fed with high-fat diet for 8 weeks and control group was fed with normal diet .The rats of model group with body weight ≥20%higher than those of the control group were matched and their female offsprings were taken as model offspring group .The female offsprings of the parent control group were taken as control offspring group .The 21-days old immature rat model group was fed with high-fat diet for 5 weeks while the control group immature rats were fed with normal diet for the same time.Since 21-days old the immature rats were observed for the vaginal opening state daily , and after the vaginal opening they were examined by vaginal smear .The rats were weighed daily , and their abdominal circumference and body length were measured biweekly.The rats of both experimental groups were killed at the age of 56-days old, the serum triglyceride, cholesterol, estradiol, follicle stimulating hormone, and luteinizing hormone were measured , and the uterus, ovaries, pituitary gland were taken and weighed and the ovary index and uterus index were calculated .The uterus and ovaries were examined by pathology using HE staining , and the corpus luteum and mature follicles in the largest cross section of ovaries werecounted.Results 1.Thebodyweightandtheabdominalcircumferenceofthe56-daysoldimmatureratsinthe model group were significantly higher than those of control group (P<0.001).2.The values of serum TG and CH in the immature rats of model group were significantly higher than those in the control group (P<0.001).3.The values of serum LH and E2 in the immature rats of model group were significantly higher , and the value of serum FSH was significantly lower than those of the control group (P<0.001 for all).4.The number of corpus luteum in the largest cross section of ovaries was significantly higher , and the number of mature follicles was significantly lower in the model group than those of control group (P<0.001 for both).5.The Pearson correlation analysis showed that the body weight of immature rats was positively related to the serum LH level and quantity of corpus luteum , and negatively correlated with vaginal opening time and the first diestrus time; the abdominal perimeter of the immature rats was positively related to the quantity of corpus luteum, serum LH, and negatively correlated with vaginal opening time and the first diestrus time .Conclusions The early puberty of immature SD rats is positively related to obesity , i.e.the development of immature rats with obesity occurs earlier than in normal immature rats .