CAJ | 학술논문

目的：研究长爪沙鼠非酒精性脂肪性肝病（ NAFLD）形成中氧化应激及细胞因子的动态变化及意义。方法48只雄性长爪沙鼠随机分为正常组和模型组，每组24只，分别给予普通饲料和高脂饲料喂养，在实验第4周、第8周和第16周末每组分别处理8只沙鼠，化学法动态检测肝组织匀浆MDA含量以及SOD、GSH－PX和T－AOC活力，液相悬浮芯片技术动态检测血清TNF－α、INF－γ、IL－10等细胞因子水平。结果随NAFLD疾病的进展，沙鼠肝组织MDA含量逐渐增加，且均较正常组明显增高（ P＜0．01）；T－AOC水平先略升高后降低，4周、16周时较正常组显著下降（P＜0．05）；SOD水平先显著增高后明显降低，4周时较正常组明显增高（P＜0．05），8周、16周时明显降低（P＜0．05、P＜0．01）；GSH－PX水平呈逐渐下降趋势，8周、16周时较正常组明显降低（P＜0．05）。血清细胞因子TNF－α、IFN－γ随NAFLD进展逐渐增加，IL－10水平则逐渐降低，在8周、16周时与正常组有统计学差异（ P＜0．05、P＜0．01）。结论高脂诱导的长爪沙鼠NAFLD模型氧化应激相关指标及炎症细胞因子随着单纯脂肪肝向脂肪性肝炎、肝纤维化及肝硬化的发展而出现显著变化，参与NAFLD的发生和发展。
목적：연구장조사서비주정성지방성간병（ NAFLD）형성중양화응격급세포인자적동태변화급의의。방법48지웅성장조사서수궤분위정상조화모형조，매조24지，분별급여보통사료화고지사료위양，재실험제4주、제8주화제16주말매조분별처리8지사서，화학법동태검측간조직균장MDA함량이급SOD、GSH－PX화T－AOC활력，액상현부심편기술동태검측혈청TNF－α、INF－γ、IL－10등세포인자수평。결과수NAFLD질병적진전，사서간조직MDA함량축점증가，차균교정상조명현증고（ P＜0．01）；T－AOC수평선략승고후강저，4주、16주시교정상조현저하강（P＜0．05）；SOD수평선현저증고후명현강저，4주시교정상조명현증고（P＜0．05），8주、16주시명현강저（P＜0．05、P＜0．01）；GSH－PX수평정축점하강추세，8주、16주시교정상조명현강저（P＜0．05）。혈청세포인자TNF－α、IFN－γ수NAFLD진전축점증가，IL－10수평칙축점강저，재8주、16주시여정상조유통계학차이（ P＜0．05、P＜0．01）。결론고지유도적장조사서NAFLD모형양화응격상관지표급염증세포인자수착단순지방간향지방성간염、간섬유화급간경화적발전이출현현저변화，삼여NAFLD적발생화발전。
ObjectiveToexplorethedynamicchangesofoxidativestressandcytokinesinMongoliangerbilswith nonalcoholic fatty liver disease ( NAFLD) and their significance.Methods Forty-eight healthy male gerbils were randomly divided into normal group and model group , 24 in each group .Gerbils of the model group were fed with high fat diet while those of the normal group with normal diet .Eight gerbils in each group were killed at the end of 4 w, 8 w and 16 w, respectively .MDA content and SOD , GSH-PX and T-AOC activity in the liver tissue were detected by chemical method, and serum TNF-α, INF-γand IL-10 levels were determined using liquid suspension chip .Results With the development of NAFLD , MDA content in liver increased gradually , and the MDA contents were all significantly higher than those of the normal group ( P<0.01 ); T-AOC level slightly increased , and then decreased , the levels at 4 w and 16 w were markedly decreased compared with those of the normal group (P<0.05);SOD level was significantly increased and then markedly reduced, the level of the model group at 4 w was significantly increased (P<0.05), while that at 8 w and 16 w were significantly decreased (P<0.05, P<0.01).The level of GSH-PX was decreased gradually , the levels at 8 w and 16 w were significantly lower than those of the normal group (P<0.05).With the progression of NAFLD,serum TNF-αand IFN-γwere increased gradually , while the level of IL-10 decreased gradually , and the levels at 8 w and 16 w were significantly lower than those of the normal group ( P <0.05, P <0.01).Conclusions The oxidative stress-related indicators and inflammatory cytokines in the gerbil NAFLD models induced by high fat diet are significantly changed as simple fatty liver develops into steatohepatitis , liver fibrosis and cirrhosis , and participate in the development and progression of NAFLD .