CAJ | 학술논문

目的：通过观察不同阶段各实验组小鼠血清中免疫球蛋白（ IgE ）及肺组织中超氧化物歧化酶（ SOD）活力与抑制率的变化，探讨越婢加半夏汤对过敏性哮喘小鼠的作用机制。方法随机将健康昆明种小白鼠分为正常对照组、哮喘模型组、地塞米松干预组和中药制剂组。采用卵白蛋白（ OVA）、氢氧化铝凝胶混悬液腹腔注射致敏和雾化激发的方法建立小鼠哮喘模型，造模成功后，中药制剂组给予越婢加半夏溶液1 mL／100 g。地塞米松组给予腹腔注射地塞米松1 mg ／kg。正常对照组和哮喘模型组小鼠分次给予生理盐水灌胃治疗。给药30 d后处死，检测各组小鼠血清IgE水平和肺组织SOD活力，并进行HE染色观察肺组织的病理学变化。结果中药制剂组小鼠与哮喘模型组小鼠相比，前者外周血清中IgE含量下降（P＜0．05），IgE在2组间差异有统计学意义（P＜0．05）；前者肺组织匀浆中SOD活力与抑制率升高，SOD活力与抑制率在2组间差异有统计学意义（ P＜0．05）。结论降低过敏性哮喘小鼠血清中IgE含量及升高小鼠肺组织SOD活力与抑制率水平，提高肺组织抗氧化能力，可能为越婢加半夏汤治疗过敏性哮喘的作用机制之一。
목적：통과관찰불동계단각실험조소서혈청중면역구단백（ IgE ）급폐조직중초양화물기화매（ SOD）활력여억제솔적변화，탐토월비가반하탕대과민성효천소서적작용궤제。방법수궤장건강곤명충소백서분위정상대조조、효천모형조、지새미송간예조화중약제제조。채용란백단백（ OVA）、경양화려응효혼현액복강주사치민화무화격발적방법건립소서효천모형，조모성공후，중약제제조급여월비가반하용액1 mL／100 g。지새미송조급여복강주사지새미송1 mg ／kg。정상대조조화효천모형조소서분차급여생리염수관위치료。급약30 d후처사，검측각조소서혈청IgE수평화폐조직SOD활력，병진행HE염색관찰폐조직적병이학변화。결과중약제제조소서여효천모형조소서상비，전자외주혈청중IgE함량하강（P＜0．05），IgE재2조간차이유통계학의의（P＜0．05）；전자폐조직균장중SOD활력여억제솔승고，SOD활력여억제솔재2조간차이유통계학의의（ P＜0．05）。결론강저과민성효천소서혈청중IgE함량급승고소서폐조직SOD활력여억제솔수평，제고폐조직항양화능력，가능위월비가반하탕치료과민성효천적작용궤제지일。
ObjectiveToinvestigatetheeffectandmechanismofaChinesetraditionalprescription,YuebiBanxia decoction, on allergic asthma in mice by observing the changes of serum immunoglobulin E ( IgE ) and lung tissue superoxide dismutase ( SOD) activity in the mouse model of allergic asthma .Methods Forty healthy Kunming mice were randomly divided into normal control group , asthma model group , dexamethasone intervention group and Chinese medicine preparation group .The mouse model of asthma was generated by peritoneal injection of ovalbumin ( OVA) and suspension of aluminium hydroxide .The mice of traditional Chinese medicine group received Yuebi Banxia decoction in a dose of 1 mL/100 g, daily for 30 days.The mice of dexamethasone group were given intraperitoneal injection of dexamethasone 1 mg/kg.The normal control group and asthma model group were given gastric gavage of physiological saline.After 30 days administration, the serum levels of IgE and SOD activity of lung tissue were determined , and the pathological changes of lung tissue were observed using HE staining .Results The mice of traditional Chinese medicine preparation group showed significantly decreased serum IgE level , and significantly increased SOD activity than those of the asthma model group mice (P<0.05 for both).Conclusions Reduced serum IgE and increased SOD activity in the lung tissue of mice with allergic asthma , improving the antioxidant ability of lung tissue , may be involved in the anti-allergic asthma mechanism of the Chinese traditional prescription Yuebi Banxia decoction .