中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
Chinese Journal of Biomedical Engineering
2015年
4期
330-335
,共6页
周泽荣%刘风华%黄倩文%阮建兴%张丽霞%李玲
週澤榮%劉風華%黃倩文%阮建興%張麗霞%李玲
주택영%류풍화%황천문%원건흥%장려하%리령
前动力蛋白-1%孕激素受体%反复种植失败%黄体中期%子宫内膜
前動力蛋白-1%孕激素受體%反複種植失敗%黃體中期%子宮內膜
전동력단백-1%잉격소수체%반복충식실패%황체중기%자궁내막
Prokineticin - 1%Progesterone receptor%Repeated implantation failure%Mid - luteal phase%Endometrium
目的探讨前动力蛋白?1（PROK1）和孕激素受体（PR）在反复种植失败（RIF）患者黄体中期子宫内膜中的表达及作用。方法从2013年8月至2014年4月到本院生殖中心行体外受精?胚胎移植助孕的患者中选取21例RIF患者为实验组，23例曾孕妇女为对照组，通过实时荧光定量PCR （RTq?PCR）法和免疫组织化学法对两组人群黄体中期子宫内膜PROK1和PR的mRNA及蛋白的相对表达水平进行检测。结果在实验组和对照组子宫内膜上均可见PROK1和PR蛋白表达，PROK1蛋白表达于腺上皮细胞、内皮细胞和基质细胞，定位于胞质，PR蛋白亦表达于腺上皮细胞、内皮细胞和基质细胞，但定位于胞核。实验组PROK1的mRNA和蛋白表达量明显低于对照组（mRNA：0.21±0.20比0.36±0.18，蛋白：0.016±0.006比0.021±0.004，均P<0.05）；实验组PR的mRNA和蛋白表达量也明显低于对照组（mRNA：2.49±0.87比4.28±1.22，蛋白：0.27±0.03比0.34±0.02，均P<0.05）。结论 PROK1和PR在RIF患者黄体中期子宫内膜的相对低表达可能是引起RIF的分子机制之一。
目的探討前動力蛋白?1（PROK1）和孕激素受體（PR）在反複種植失敗（RIF）患者黃體中期子宮內膜中的錶達及作用。方法從2013年8月至2014年4月到本院生殖中心行體外受精?胚胎移植助孕的患者中選取21例RIF患者為實驗組，23例曾孕婦女為對照組，通過實時熒光定量PCR （RTq?PCR）法和免疫組織化學法對兩組人群黃體中期子宮內膜PROK1和PR的mRNA及蛋白的相對錶達水平進行檢測。結果在實驗組和對照組子宮內膜上均可見PROK1和PR蛋白錶達，PROK1蛋白錶達于腺上皮細胞、內皮細胞和基質細胞，定位于胞質，PR蛋白亦錶達于腺上皮細胞、內皮細胞和基質細胞，但定位于胞覈。實驗組PROK1的mRNA和蛋白錶達量明顯低于對照組（mRNA：0.21±0.20比0.36±0.18，蛋白：0.016±0.006比0.021±0.004，均P<0.05）；實驗組PR的mRNA和蛋白錶達量也明顯低于對照組（mRNA：2.49±0.87比4.28±1.22，蛋白：0.27±0.03比0.34±0.02，均P<0.05）。結論 PROK1和PR在RIF患者黃體中期子宮內膜的相對低錶達可能是引起RIF的分子機製之一。
목적탐토전동력단백?1（PROK1）화잉격소수체（PR）재반복충식실패（RIF）환자황체중기자궁내막중적표체급작용。방법종2013년8월지2014년4월도본원생식중심행체외수정?배태이식조잉적환자중선취21례RIF환자위실험조，23례증잉부녀위대조조，통과실시형광정량PCR （RTq?PCR）법화면역조직화학법대량조인군황체중기자궁내막PROK1화PR적mRNA급단백적상대표체수평진행검측。결과재실험조화대조조자궁내막상균가견PROK1화PR단백표체，PROK1단백표체우선상피세포、내피세포화기질세포，정위우포질，PR단백역표체우선상피세포、내피세포화기질세포，단정위우포핵。실험조PROK1적mRNA화단백표체량명현저우대조조（mRNA：0.21±0.20비0.36±0.18，단백：0.016±0.006비0.021±0.004，균P<0.05）；실험조PR적mRNA화단백표체량야명현저우대조조（mRNA：2.49±0.87비4.28±1.22，단백：0.27±0.03비0.34±0.02，균P<0.05）。결론 PROK1화PR재RIF환자황체중기자궁내막적상대저표체가능시인기RIF적분자궤제지일。
Objective To investigate the expression and role of prokineticin?1 (PROK1) and progesterone receptor (PR) in mid?secretory phase endometrium of patients with repeated implantation failure(RIF). Methods From subjects who visited reproductive center for in?vitro fertilization and embryo transfer between August 2013 and April 2014,21 subjects with RIF were selected as the study group,and 23 pregnant women as the control group. Real?time quantitative PCR(RTq?PCR)and immunohistochemistry were used to measure the mRNA and protein relative expression levels of PROK1 and PR in mid?secretory phase endometrium of the two groups. Results Expression of PROK1 and PR protein was noted in endometrium of the study and control groups. The PROK1 proteins were expressed in epithelial,endothelial and stromal cells,and located in the cytoplasm;the PR proteins were also expressed in epithelial, endothelial and stromal cells,but localized in the nucleus. The mRNA and protein expression levels of PROK1 were significantly lower in the study group than in the control group(mRNA:0.21 ± 0.20 vs 0.36 ± 0.18,protein:0.016 ± 0.006 vs 0.021 ± 0.004,all P<0.05),and so were the mRNA and protein expression levels of PR in the study group compared with the control group(mRNA:2.49±0.87 vs 4.28±1.22,protein:0.27 ± 0.03 vs 0.34 ± 0.02,all P<0.05).Conclusion Relatively low PROK1 and PR expression in mid?secretory endometrium of RIF patients may be one of the molecular mechanisms underlying RIF.
