CAJ | 학술논문

目的：探讨重楼皂苷Ⅶ联合顺铂抑制荷瘤小鼠移植瘤生长的分子机制。方法 SKOV3细胞皮下接种NOD／SCID 小鼠，待移植瘤形成后，荷瘤小鼠随机分为4组（n＝10）：对照组、低剂量重楼皂苷Ⅶ组（LD 组）、中剂量重楼皂苷Ⅶ组（MD 组）、高剂量重楼皂苷Ⅶ组（HD 组）。15天后，分离肿瘤组织，测定肿瘤体积和重量及抑瘤率；TUNEL 染色和 Western blot 检测肿瘤细胞凋亡及其机制。结果在 HD 组中，肿瘤重量抑制率达57．98％，体积抑制率达55．11％，细胞凋亡率达42．39％，与对照组相比有统计学差异。经重楼皂苷作用后，凋亡相关基因 Bcl-2表达减少，Bax 蛋白表达增加；内质网相关基因 GRP78和 CHOP 表达均增加。结论重楼皂苷能有效促进肿瘤细胞凋亡，并且这种凋亡是由内质网应激介导的。
목적：탐토중루조감Ⅶ연합순박억제하류소서이식류생장적분자궤제。방법 SKOV3세포피하접충NOD／SCID 소서，대이식류형성후，하류소서수궤분위4조（n＝10）：대조조、저제량중루조감Ⅶ조（LD 조）、중제량중루조감Ⅶ조（MD 조）、고제량중루조감Ⅶ조（HD 조）。15천후，분리종류조직，측정종류체적화중량급억류솔；TUNEL 염색화 Western blot 검측종류세포조망급기궤제。결과재 HD 조중，종류중량억제솔체57．98％，체적억제솔체55．11％，세포조망솔체42．39％，여대조조상비유통계학차이。경중루조감작용후，조망상관기인 Bcl-2표체감소，Bax 단백표체증가；내질망상관기인 GRP78화 CHOP 표체균증가。결론중루조감능유효촉진종류세포조망，병차저충조망시유내질망응격개도적。
Objective To investigate the molecular mechanism of inhibiting of tumor growth in tumor-bearing mice by polyphyllin Ⅶ and cisplatin.Methods SKOV3 cells were inoculated subcutaneously into NOD/SCID mice,and the tumor-bearing mice were randomly divided into four groups (n = 10):the control group,low-dose of polyphyllin Ⅶgroup (group LD),medium-dose of polyphyllin Ⅶ group (group MD),high-dose of polyphyllin Ⅶ group (group HD). 15 days later,the tumor tissues were isolated and measured volume and weight to calculate the tumor inhibition rate;the tumor cell apoptosis and its mechanism were determined by TUNEL staining and Western blot.Results In group HD, the weight inhibition rate was 57.98%,volume inhibition rate was 55.11%,and the apoptotic rate was 42.39%,there was a significant difference compared with the control group.After treatment of polyphyllin Ⅶ,the expression of Bcl-2 was decreased and the expression of Bax was increased;the expression of GRP78 and CHOP,the endoplasmic reticu-lum-related gene,was increased too.Conclusion The polyphyllins could effectively promote the apoptosis of tumor cells via endoplasmic reticulum stress.