中国实验诊断学
中國實驗診斷學
중국실험진단학
Chinese Journal of Laboratory Diagnosis
2015年
9期
1444-1447
,共4页
银杏叶提取物%尾加压素Ⅱ%心肌成纤维细胞%胶原
銀杏葉提取物%尾加壓素Ⅱ%心肌成纖維細胞%膠原
은행협제취물%미가압소Ⅱ%심기성섬유세포%효원
ginkgobioba extract%urotensin Ⅱ%cardiac fibroblasts%collagen
目的:探讨银杏叶提取物(GBE)对尾加压素Ⅱ(U Ⅱ)介导的成纤维细胞(CFs)过度合成和分泌 ECM 的抑制作用。方法以 U Ⅱ刺激原代培养的新生大鼠心肌 CFs,同时加入 GBE 进行干预,以比色法测定培养上清中的羟脯氨酸含量,以 ELISA 法测定培养细胞中Ⅰ型胶原(Col Ⅰ)含量,以 Real-time PCR 法测定各组细胞中 TGF-β和 CT-GF mRNA 表达。结果与正常对照组比较,U Ⅱ处理组细胞培养上清中羟脯氨酸及 Col I 含量显著增加(P <0.05);与 U Ⅱ处理组比较,GBE 高、中剂量组培养上清中羟脯氨酸及 Col I 含量均显著降低(P <0.05),GBE 低剂量组羟脯氨酸含量亦较 U Ⅱ处理组明显降低(P <0.05);Real-time PCR 结果显示,与正常对照组比较,U Ⅱ处理组细胞TGF-β及 CTGF mRNA 表达显著增加(P <0.05);与 U Ⅱ处理组比较,GBE 高、中、低3个剂量组 TGF-β表达量均下降(P <0.05),而对 CTGF,仅高剂量组有明显统计学意义(P <0.05)。结论GBE 抑制 CFs 胶原过度合成和分泌作用可能是通过其抑制 TGF-β、CTGF 途径实现的。
目的:探討銀杏葉提取物(GBE)對尾加壓素Ⅱ(U Ⅱ)介導的成纖維細胞(CFs)過度閤成和分泌 ECM 的抑製作用。方法以 U Ⅱ刺激原代培養的新生大鼠心肌 CFs,同時加入 GBE 進行榦預,以比色法測定培養上清中的羥脯氨痠含量,以 ELISA 法測定培養細胞中Ⅰ型膠原(Col Ⅰ)含量,以 Real-time PCR 法測定各組細胞中 TGF-β和 CT-GF mRNA 錶達。結果與正常對照組比較,U Ⅱ處理組細胞培養上清中羥脯氨痠及 Col I 含量顯著增加(P <0.05);與 U Ⅱ處理組比較,GBE 高、中劑量組培養上清中羥脯氨痠及 Col I 含量均顯著降低(P <0.05),GBE 低劑量組羥脯氨痠含量亦較 U Ⅱ處理組明顯降低(P <0.05);Real-time PCR 結果顯示,與正常對照組比較,U Ⅱ處理組細胞TGF-β及 CTGF mRNA 錶達顯著增加(P <0.05);與 U Ⅱ處理組比較,GBE 高、中、低3箇劑量組 TGF-β錶達量均下降(P <0.05),而對 CTGF,僅高劑量組有明顯統計學意義(P <0.05)。結論GBE 抑製 CFs 膠原過度閤成和分泌作用可能是通過其抑製 TGF-β、CTGF 途徑實現的。
목적:탐토은행협제취물(GBE)대미가압소Ⅱ(U Ⅱ)개도적성섬유세포(CFs)과도합성화분비 ECM 적억제작용。방법이 U Ⅱ자격원대배양적신생대서심기 CFs,동시가입 GBE 진행간예,이비색법측정배양상청중적간포안산함량,이 ELISA 법측정배양세포중Ⅰ형효원(Col Ⅰ)함량,이 Real-time PCR 법측정각조세포중 TGF-β화 CT-GF mRNA 표체。결과여정상대조조비교,U Ⅱ처리조세포배양상청중간포안산급 Col I 함량현저증가(P <0.05);여 U Ⅱ처리조비교,GBE 고、중제량조배양상청중간포안산급 Col I 함량균현저강저(P <0.05),GBE 저제량조간포안산함량역교 U Ⅱ처리조명현강저(P <0.05);Real-time PCR 결과현시,여정상대조조비교,U Ⅱ처리조세포TGF-β급 CTGF mRNA 표체현저증가(P <0.05);여 U Ⅱ처리조비교,GBE 고、중、저3개제량조 TGF-β표체량균하강(P <0.05),이대 CTGF,부고제량조유명현통계학의의(P <0.05)。결론GBE 억제 CFs 효원과도합성화분비작용가능시통과기억제 TGF-β、CTGF 도경실현적。
Objective To determine the prevention effects of ginkgobioba extract (GBE)on urotensin Ⅱ (U Ⅱ)in-duced over-expression of extracellular matrix (ECM).Methods The U Ⅱ was used to stimulus the cardiac fibroblast of the new born rats,and the GBE were added.The colorimetry was applied to determine the content of hydroxyproline and collgen I were detected by ELISA in various group.The expression of TGF-β and CTGF were evaluated by real-time PCR.Results Both hydroxyproline and collgen I in the U Ⅱ-treated group were higher significantly than the con-trol group (P <0.05,that in the high-and middle-dose of GBE group were decreased significantly vs the U Ⅱ-treated group (P <0.05),the hydroxyproline in the low-dose of GBE group were also decreased markedly (P <0.05).The re-sults of real-time PCR showed that compared with the control group,the TGF-β and CTGF mRNA is obviously in-creased (P <0.05),and the TGF-β mRNA level in all the three group and the CTGF mRNA level in the high-dose group were decreased significantly (P <0.05).Conclusion The GBE can depress the over expression of ECM in cardi-ac fibroblasts which induced by U Ⅱ,and the mechanism maybe have relationship of with TGF-βand CTGF.