中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
Chinese Journal of Biomedical Engineering
2015年
4期
291-295
,共5页
高嵩涛%蔡启卿%姚伟涛%王家强%王鑫%张鹏
高嵩濤%蔡啟卿%姚偉濤%王傢彊%王鑫%張鵬
고숭도%채계경%요위도%왕가강%왕흠%장붕
脂肪干细胞%许旺细胞%神经再生%组织工程
脂肪榦細胞%許旺細胞%神經再生%組織工程
지방간세포%허왕세포%신경재생%조직공정
Adipose derived stem cells%Schwann cells%Nerve regeneration%Tissue engineering
目的：探讨去细胞同种异体神经复合脂肪干细胞（ADSC）诱导分化的类许旺细胞在修复大鼠坐骨神经缺损中的效果。方法健康F344近交系大鼠30只，随机分为2组，每组15只，分别用不同的移植物桥接10 mm坐骨神经缺损，A组用单纯去细胞同种异体神经（SD大鼠去细胞神经支架）桥接，B组用脂肪干细胞诱导分化的类许旺细胞复合去细胞同种异体神经桥接。比较两组术后6、12周坐骨神经功能指数恢复率（SFI%）、神经电生理（NEP）包括坐骨神经传导速度（MNCV）恢复率和小腿腓肠肌复合动作电位（CAMP）恢复率、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定、组织学光镜和透射电镜观察髓鞘再生情况等指标的变化，评价实验效果。结果术后6、12周，B组SFI%（6周时52.38±7.12，12周时79.99±10.33）、MNCV恢复率（6周时51.12±8.15，12周时75.93±5.95）、CAMP恢复率（6周时45.38±4.12，12周时75.98±10.99）、再生有髓神经纤维计数恢复率（6周时44.29±7.52，12周时77.06±11.54）、神经纤维直径恢复率（6周时43.58±4.87，12周时83.23±6.32）、髓鞘厚度恢复率（6周时46.41±4.35，12周时83.96±8.31）等指标均优于A组SFI%（6周时46.32±5.13，12周时72.73±8.06）、MNCV恢复率（6周时42.54±6.33，12周时69.34±9.13）、CAMP恢复率（6周时40.52±4.15，12周时68.24±9.45）、再生有髓神经纤维计数恢复率（6周时38.45±6.28，12周时67.89±11.87）、神经纤维直径恢复率（6周时40.11±4.32，12周时76.37±9.38）、髓鞘厚度恢复率（6周时41.33±4.58，12周时77.62±7.98）（均P<0.05）。B组神经组织学光镜观察可见神经纤维直径、数量及髓鞘厚度优于A组，透射电镜观察可见B组神经组织较A组髓鞘排列致密，髓鞘较厚。结论脂肪干细胞诱导分化的类许旺细胞复合去细胞同种异体神经构建的组织工程化神经能有效地修复大鼠坐骨神经缺损。
目的：探討去細胞同種異體神經複閤脂肪榦細胞（ADSC）誘導分化的類許旺細胞在脩複大鼠坐骨神經缺損中的效果。方法健康F344近交繫大鼠30隻，隨機分為2組，每組15隻，分彆用不同的移植物橋接10 mm坐骨神經缺損，A組用單純去細胞同種異體神經（SD大鼠去細胞神經支架）橋接，B組用脂肪榦細胞誘導分化的類許旺細胞複閤去細胞同種異體神經橋接。比較兩組術後6、12週坐骨神經功能指數恢複率（SFI%）、神經電生理（NEP）包括坐骨神經傳導速度（MNCV）恢複率和小腿腓腸肌複閤動作電位（CAMP）恢複率、再生有髓神經纖維計數恢複率、神經纖維直徑恢複率、髓鞘厚度恢複率的測定、組織學光鏡和透射電鏡觀察髓鞘再生情況等指標的變化，評價實驗效果。結果術後6、12週，B組SFI%（6週時52.38±7.12，12週時79.99±10.33）、MNCV恢複率（6週時51.12±8.15，12週時75.93±5.95）、CAMP恢複率（6週時45.38±4.12，12週時75.98±10.99）、再生有髓神經纖維計數恢複率（6週時44.29±7.52，12週時77.06±11.54）、神經纖維直徑恢複率（6週時43.58±4.87，12週時83.23±6.32）、髓鞘厚度恢複率（6週時46.41±4.35，12週時83.96±8.31）等指標均優于A組SFI%（6週時46.32±5.13，12週時72.73±8.06）、MNCV恢複率（6週時42.54±6.33，12週時69.34±9.13）、CAMP恢複率（6週時40.52±4.15，12週時68.24±9.45）、再生有髓神經纖維計數恢複率（6週時38.45±6.28，12週時67.89±11.87）、神經纖維直徑恢複率（6週時40.11±4.32，12週時76.37±9.38）、髓鞘厚度恢複率（6週時41.33±4.58，12週時77.62±7.98）（均P<0.05）。B組神經組織學光鏡觀察可見神經纖維直徑、數量及髓鞘厚度優于A組，透射電鏡觀察可見B組神經組織較A組髓鞘排列緻密，髓鞘較厚。結論脂肪榦細胞誘導分化的類許旺細胞複閤去細胞同種異體神經構建的組織工程化神經能有效地脩複大鼠坐骨神經缺損。
목적：탐토거세포동충이체신경복합지방간세포（ADSC）유도분화적류허왕세포재수복대서좌골신경결손중적효과。방법건강F344근교계대서30지，수궤분위2조，매조15지，분별용불동적이식물교접10 mm좌골신경결손，A조용단순거세포동충이체신경（SD대서거세포신경지가）교접，B조용지방간세포유도분화적류허왕세포복합거세포동충이체신경교접。비교량조술후6、12주좌골신경공능지수회복솔（SFI%）、신경전생리（NEP）포괄좌골신경전도속도（MNCV）회복솔화소퇴비장기복합동작전위（CAMP）회복솔、재생유수신경섬유계수회복솔、신경섬유직경회복솔、수초후도회복솔적측정、조직학광경화투사전경관찰수초재생정황등지표적변화，평개실험효과。결과술후6、12주，B조SFI%（6주시52.38±7.12，12주시79.99±10.33）、MNCV회복솔（6주시51.12±8.15，12주시75.93±5.95）、CAMP회복솔（6주시45.38±4.12，12주시75.98±10.99）、재생유수신경섬유계수회복솔（6주시44.29±7.52，12주시77.06±11.54）、신경섬유직경회복솔（6주시43.58±4.87，12주시83.23±6.32）、수초후도회복솔（6주시46.41±4.35，12주시83.96±8.31）등지표균우우A조SFI%（6주시46.32±5.13，12주시72.73±8.06）、MNCV회복솔（6주시42.54±6.33，12주시69.34±9.13）、CAMP회복솔（6주시40.52±4.15，12주시68.24±9.45）、재생유수신경섬유계수회복솔（6주시38.45±6.28，12주시67.89±11.87）、신경섬유직경회복솔（6주시40.11±4.32，12주시76.37±9.38）、수초후도회복솔（6주시41.33±4.58，12주시77.62±7.98）（균P<0.05）。B조신경조직학광경관찰가견신경섬유직경、수량급수초후도우우A조，투사전경관찰가견B조신경조직교A조수초배렬치밀，수초교후。결론지방간세포유도분화적류허왕세포복합거세포동충이체신경구건적조직공정화신경능유효지수복대서좌골신경결손。
Objective To investigate the efficacy of allogenic acellular nerve scaffold and adipose derived stem cells (ADSCs)?differentiated Schwann?like in repairing the sciatic nerve defect of rats. Methods Thirty healthy F344 inbred rats were randomly divided into two groups (n=15 each). In these groups,a 10?mm sciatic nerve defect was bridged with different grafts:allogenic acellular nerve(from SD rat)scaffold in Group A,and allogenic acellular nerve scaffold seeded with Schwann?like cells differentiated from ADSCs in Group B. After 6 and 12 weeks,the both groups were compared for recovery rate of sciatic functional index (SFI%) and the neural electrophysiology (NEP) which included recovery rates of motor nerve conduction velocity (MNCV) of sciatic nerve, compound muscle action potential (CAMP) of gastrocnemius,regenerated myelinated fiber counts,nerve fiber diameter and myelin sheath thickness,as well as histological changes with myelin regeneration under light microscopy and transmission electron microscopy,so as to evaluate the experimental efficacy. Results At 6 and 12 weeks after operation,the Group B appeared advantageous over Group A in SFI%(at 6 weeks,52.38 ± 7.12 vs 46.32 ± 5.13;at 12 weeks,79.99 ± 10.33 vs 72.73 ± 8.06),MNCV recovery rate(at 6 weeks,51.12 ± 8.15 vs 42.54 ± 6.33;at12 weeks,75.93 ± 5.95 vs 69.34 ± 9.13),CAMP recovery rate(at 6 weeks,45.38 ± 4.12 vs 40.52 ± 4.15;at 12 weeks,75.98 ± 10.99 vs 68.24 ± 9.45),regenerated myelinated nerve fiber count recovery rate(at 6 weeks, 44.29±7.52 vs 38.45±6.28;at 12 weeks,77.06±11.54 vs 67.89±11.87),nerve fiber diameter recovery rate (at 6 weeks,43.58 ± 4.87 vs 40.11 ± 4.32;at 12 weeks,83.23 ± 6.32 vs 76.37 ± 9.38),myelin sheath thickness recovery rate(at 6 weeks,46.41±4.35 vs 41.33±4.58;at 12 weeks,83.96±8.31 vs 77.62±7.98) (all P<0.05). Light microscopy revealed that the diameter and number of nerve fiber and the myelin sheath thickness were more favorable in Group B than in Group A. Transmission electron microscopy revealed that the nerve tissue was more densely arranged and with thicker myelin in Group B than as found for Group A. Conclusion Tissue?engineered nerve constructed from ADSCs?differentiated Schwann?like cells combined with allogenic acellular nerve can effectively repair nerve defects in rats.
