CAJ | 학술논문

目的：探讨Aurora-A的表达与胰腺癌吉西他滨耐药的相关性。方法 qPCR法测定PANC-1和耐药细胞株PANC-1/R2中Aurora-A mRNA的表达；分别用PANC-1和PANC-1/R2建立胰腺癌动物模型，免疫组化方法测定瘤体Aurora-A的表达。结果 PANC-1组模型第5周肿瘤转移率36.8%（7/19），裸鼠体重（23.2±1.41）g，肿瘤重量（0.453±0.110）g；PANC-1/R2组模型第5周肿瘤转移率50%（9/18），裸鼠体重（22.91±1.13）g，肿瘤重量（0.564±0.203）g。转移率和肿瘤质量两组比较差异有统计学意义。PANC-1组Aurora-A mRNA表达为（1.002±0.040）；PANC-1/R2组Aurora-A mRNA表达为（1.845±0.069），两组比较差异有统计学意义（P<0.05）。PANC-1/R2组18例标本组织中Aurora-A蛋白阳性表达15例（83.3%）；PANC-1组19例组织的Aurora-A蛋白检测阳性表达11例（57.9%），两组比较差异有统计学意义（P<0.05）。结论 Aurora-A基因高表达与胰腺癌细胞株的吉西他滨耐药有关，新方法构建的PANC-1/R2耐药株Aurora-A基因高表达，可用于胰腺癌吉西他滨耐药研究的载体。
목적：탐토Aurora-A적표체여이선암길서타빈내약적상관성。방법 qPCR법측정PANC-1화내약세포주PANC-1/R2중Aurora-A mRNA적표체；분별용PANC-1화PANC-1/R2건립이선암동물모형，면역조화방법측정류체Aurora-A적표체。결과 PANC-1조모형제5주종류전이솔36.8%（7/19），라서체중（23.2±1.41）g，종류중량（0.453±0.110）g；PANC-1/R2조모형제5주종류전이솔50%（9/18），라서체중（22.91±1.13）g，종류중량（0.564±0.203）g。전이솔화종류질량량조비교차이유통계학의의。PANC-1조Aurora-A mRNA표체위（1.002±0.040）；PANC-1/R2조Aurora-A mRNA표체위（1.845±0.069），량조비교차이유통계학의의（P<0.05）。PANC-1/R2조18례표본조직중Aurora-A단백양성표체15례（83.3%）；PANC-1조19례조직적Aurora-A단백검측양성표체11례（57.9%），량조비교차이유통계학의의（P<0.05）。결론 Aurora-A기인고표체여이선암세포주적길서타빈내약유관，신방법구건적PANC-1/R2내약주Aurora-A기인고표체，가용우이선암길서타빈내약연구적재체。
ObjectiveTo assess the relationship of Aurora-A expression with gemcitabine-resistance of pancreatic cancer.MethodsThe expression of Aurora-A mRNA of PANC-1 and PANC-1/R2 cells were tested by qPCR technique. The animal models of pancreatic cancer were estblised by PANC-1 and PANC-1/R2 cells. After 35days,mice were killed,and the tumor tissues were collected. The Aurora-A protein expression of the two groups were deteched by Immunohistochemistry.ResultsIn the PANC-1 group,the metastasis of tumor on the five weeks was 36.8%(7/19), the mean weight of tumor tissues was 0.453±0.110 g,and weight of mice was 23.2±1.41 g. While the metastasis of tumor on the five weeks was 50%(9/18),the mean weight of tumor tissues was 0.564±0.203g,and weight of mice was 22.91±1.13g in PANC-1/R2 group. The metastatic rate and weight of tumor were with significant differences(P<0.05). The expression of Aurora-A mRNA of PANC-1 cells was 1.002±0.040;while it was 1.845±0.069 in PANC-1/R2,it was significant difference between the two groups(P<0.05). A 15 of 18 cases positive expression of Aurora-A protein were detected in PANC-1/R2 group,while it was 11 of 19 cases in PANC-1 group. The significant difference was found between the two groups (P<0.05).Conclusions High expression of Aurora-A gene is associated with gemcitabine- resistance of pancreatic cancer cell lines. PANC-1/R2 cell was successful as a cell model of gemcitabine-resistance of pancreatic cancer.