CAJ | 학술논문

目的：研究川芎嗪对佐剂性关节炎（ AA）大鼠滑膜细胞的凋亡诱导作用并探讨其治疗AA的可能机制。方法弗氏完全佐剂注射SD大鼠后足跖皮内制备AA模型；用3个不同剂量（30，60，90 mg· kg－1）川芎嗪对AA模型进行干预，分离并培养滑膜细胞。 MTT法检测滑膜细胞的增殖能力，流式细胞术检测滑膜细胞的凋亡率，免疫印迹法检测Bcl －2和Bax蛋白表达。结果与模型组相比，川芎嗪可剂量依赖性地降低AA大鼠滑膜细胞的增殖；川芎嗪能下调 Bcl－2蛋白的表达，上调Bax蛋白表达，同时升高滑膜细胞的凋亡率。结论川芎嗪可抑制AA大鼠滑膜细胞的异常增殖，诱使滑膜细胞凋亡，其机制可能与调控Bcl－2和Bax蛋白有关。
목적：연구천궁진대좌제성관절염（ AA）대서활막세포적조망유도작용병탐토기치료AA적가능궤제。방법불씨완전좌제주사SD대서후족척피내제비AA모형；용3개불동제량（30，60，90 mg· kg－1）천궁진대AA모형진행간예，분리병배양활막세포。 MTT법검측활막세포적증식능력，류식세포술검측활막세포적조망솔，면역인적법검측Bcl －2화Bax단백표체。결과여모형조상비，천궁진가제량의뢰성지강저AA대서활막세포적증식；천궁진능하조 Bcl－2단백적표체，상조Bax단백표체，동시승고활막세포적조망솔。결론천궁진가억제AA대서활막세포적이상증식，유사활막세포조망，기궤제가능여조공Bcl－2화Bax단백유관。
Objective To investigate the effect of tetramethylpyrazine ( TMP) on synovial cells of adjuvant arthritis ( AA ) , and the possible mechanism in the treatment of AA.Methods Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund adjuvant into the right hind feet pads of the Spraugue Dawley(SD ) rats.AA rats were treated with various concentrations (30, 60,90 mg· kg -1 ) of TMP and collect the synovial cells.The prolifera-tion of synovial cells was measured by Methyl thiazolyl tetrazolium assay ( MTT).The protein expressions of Bcl -2 and Bax in the synovial cells were detected by Western blotting.The apoptosis rates of synovial cells were evaluated using flow cytometry ( FCM ) analysis.Results Compared with the model group , TMP could inhibit the proliferation of synovial cell in a dose-dependent manner, the expression of Bcl-2 on the groups with TMP treated was significantly increased and the expression of Bax was decreased.TMP can induce the apoptosis of synovial cells in vivo.Conclusion TMP can inhibit proliferation and induce apoptosis in synovial cells and the mechanism could be most likely to involve regulating and controlling the expression of Bcl -2 and Bax.